I-Potassium dicarboxylateiwukukhula kokuqala okungewona ama-antibiotic okukhuthaza izithasiselo zokudla okugunyazwe yi-European Union.Kuyingxube ye-potassium dicarboxylate ne-formic acid ngokusebenzisa i-intermolecular hydrogen bond.Isetshenziswa kakhulu kuma-piglets kanye nezingulube zokuqeda ezikhulayo.Imiphumela yokuhlolwa kokuphakela ibonise ukuthi ukwengeza i-potassium dicarboxylate ekudleni kwezingulube kungakhuphula kakhulu ukuzuza kwesisindo sezingulube futhi kunciphise inani lokufa okubangelwa ukutheleleka ngegciwane.Ukwengeza i-potassium dicarboxylate ekudleni kwezinkomo nakho kungathuthukisa isivuno sobisi lwezinkomo.

Kulolu cwaningo, imithamo ehlukene yei-potassium dicarboxylatezengezwe ekudleni kweprotheyini ephansi i-Penaeus vannamei, ukuze kuhlolwe i-ejenti ekhuthaza ukukhula engenama-antibiotic ephumelelayo nengenabungani kwemvelo.

Izimpahla nezindlela

1.1 okuphakelayo kokuhlola

Ifomula yokuphakelayo yokuhlola kanye nemiphumela yokuhlaziywa kwamakhemikhali kukhonjisiwe kuThebula 1. Kunamaqembu amathathu okuphakelayo esivivinyweni, futhi okuqukethwe kwe-potassium dicarboxylate kungu-0%, 0.8% no-1.5% ngokulandelanayo.

1.2 izimfanzi zokuhlola

Isisindo somzimba sokuqala sika-Penaeus vannamei sasingu-(57.0 ± 3.3) mg) C. Ukuhlolwa kwahlukaniswa kwaba amaqembu amathathu anezimpinda ezintathu eqenjini ngalinye.

1.3 izindawo zokudla

Isiko lama-shrimp senziwa emakhejini anetha anencazelo engu-0.8 mx 0.8 mx 0.8 M. wonke amakheji anetha abekwe endaweni eyindilinga eyindilinga usimende (1.2 m ukuphakama, 16.0 m ububanzi).

1.4 Ukuhlolwa kokuphakela kwe-potassium formate

Amaqembu amathathu okudla (0%, 0.8% kanye ne-1.5% potassium dicarboxylate) anikezwa ngokungahleliwe eqenjini ngalinye ngemuva kokukala izingcezu ze-30 / ibhokisi.Inani lokudla lalingu-15% wesisindo somzimba sokuqala kusukela osukwini olu-1 kuya osukwini lwe-10, u-25% kusukela osukwini lwe-11 kuya osukwini lwama-30, kanye no-35% kusukela osukwini lwama-31 kuya osukwini lwama-40. Ukuhlolwa kwathatha izinsuku ezingu-40.Izinga lokushisa lamanzi liyi-22.0-26.44 ℃ kanti ubusawoti bungu-15. Ngemva kwezinsuku ezingu-40, isisindo somzimba sasikalwa futhi sibalwe, kanye nesisindo.

2.2 imiphumela

Ngokusho kokuhlolwa kokuminyana kwesitoko, ukuminyana kwesitoko bekuyizinhlanzi ezingama-30 / ibhokisi.Izinga lokusinda kweqembu elilawulayo laliyi-(92.2 ± 1.6)%, futhi izinga lokusinda kweqembu le-0.8% le-potassium diformate laliyi-100%;Nokho, izinga lokusinda lika-Penaeus vannamei lehla laya ku-(86.7 ± 5.4)%, lapho izinga lokwengeza lenyuka laya ku-1.5%.I-coefficient yokuphakelayo nayo ibonise ithrendi efanayo.

3 ingxoxo

Kulesi sivivinyo, ukwengeza i-potassium diformate kungathuthukisa ngempumelelo inzuzo yansuku zonke kanye nezinga lokusinda lika-Penaeus vannamei.Umbono ofanayo wabekwa phambili lapho wengeza i-potassium dicarboxylate ekudleni kwezingulube.Kwaqinisekiswa ukuthi ukungezwa kwe-potassium diformate engu-0.8% ekudleni kwezimfanzi kwe-Penaeus vannamei kube nomthelela ongcono wokukhula.Roth et al.(1996) uncome ukungezwa kokudla okufanelekile ekudleni kwezingulube, okwakungu-1.8% ekudleni kokuqala, u-1.2% ekudleni kokulunyulwa kanye no-0.6% ekukhuleni nasekuqedeni izingulube.

Isizathu sokuthi kungani i-potassium dicarboxylate ingakhuthaza ukukhula ukuthi i-potassium dicarboxylate ingafinyelela endaweni ebuthakathaka ye-alkaline yamathumbu ngokudla isisu sesilwane ngendlela ephelele, futhi ibole ngokuzenzakalelayo ibe yi-formic acid kanye ne-formate, ibonise umphumela oqinile we-bacteriostatic kanye ne-bactericidal, okwenza amathumbu esilwane avele " inzalo", ngaleyo ndlela ibonise umphumela okhuthaza ukukhula.