AbstractQhov kev sim tau ua los kawm txog qhov cuam tshuam ntawm diludine ntawm kev tso cov kev ua tau zoo thiab lub qe zoo hauv hens thiab mus kom ze rau cov txheej txheem ntawm cov teebmeem los ntawm kev txiav txim siab qhov ntsuas ntawm cov qe thiab cov ntshav tsis txaus 1024 ROM hens tau muab faib ua plaub pawg uas suav nrog plaub tus qauv ntawm 64. hens txhua, Cov pab pawg kho mob tau txais tib yam khoom noj basal ntxiv nrog 0, 100, 150, 200 mg / kg diludine feem rau 80 d.Cov txiaj ntsig tau raws li hauv qab no.Ntxiv ntawm diludine rau kev noj zaub mov zoo hens tso kev ua tau zoo, ntawm qhov kev kho mob 150 mg / kg yog qhov zoo tshaj plaws;nws tus nqi ntawm nteg tau nce los ntawm 11.8% (p< 0.01), qe loj hloov pauv tau txo los ntawm 10.36% (p <0 01).Cov qe hnyav tau nce ntxiv nrog qhov nce ntawm diludine ntxiv.Diludine txo cov ntshav concentration ntawm uric acid (p <0.01);Ntxiv diludine txo qis cov ntshav Ca2+thiab inorganic phosphate cov ntsiab lus, thiab nce kev ua haujlwm ntawm alkine phosphatase (ALP) ntawm cov ntshav (p< 0.05), yog li nws muaj cov txiaj ntsig tseem ceeb ntawm kev txo qis qe tawg (p <0.05) thiab qhov txawv txav (p <0.05);diludine ho nce qhov siab ntawm albumen.Haugh tus nqi (p <0.01), lub plhaub tuab thiab lub plhaub hnyav (p <0.05), 150 thiab 200mg / kg diludine kuj txo tag nrho cov roj (cholesterol) hauv qe qe (p <0 05), tab sis nce qe qe hnyav (p <0.05).Tsis tas li ntawd, diludine tuaj yeem txhim kho cov haujlwm ntawm lipase (p <0.01), thiab txo cov ntsiab lus ntawm triglyceride (TG3) (p <0.01) thiab cov roj (cholesterol) (CHL) (p <0 01) hauv cov ntshav, nws txo qis feem pua ntawm cov rog hauv plab. (p <0.01) thiab daim siab rog cov ntsiab lus (p<0.01), muaj peev xwm tiv thaiv hens los ntawm lub siab rog.Diludine nce kev ua haujlwm ntawm SOD hauv cov ntshav (p< 0 01) thaum nws tau ntxiv rau kev noj zaub mov ntau dua 30d.Txawm li cas los xij, tsis muaj qhov sib txawv tseem ceeb hauv cov dej num ntawm GPT thiab GOT ntawm cov ntshav qab zib ntawm pawg tswj thiab kho.Nws tau txiav txim siab tias diludine tuaj yeem tiv thaiv daim nyias nyias ntawm cov hlwb los ntawm oxidation
Cov lus tseem ceebDiludine;tus qaib;SOD;cov roj cholesterol;triglycerides, lipase
Lub diludine yog qhov tshiab uas tsis yog khoom noj khoom haus los tiv thaiv oxidation vitamin additive thiab muaj cov teebmeem[1-3]ntawm inhibiting oxidation ntawm cov kab mob lom thiab stabilizing cov ntaub so ntswg ntawm cov kab mob lom, thiab lwm yam. Nyob rau hauv 1970s, cov kws txawj ua liaj ua teb ntawm Latvia nyob rau hauv yav tas los Soviet Union pom tias diludine muaj cov teebmeem.[4]ntawm kev txhawb nqa kev loj hlob ntawm nqaij qaib thiab tiv thaiv khov thiab kev laus rau qee cov nroj tsuag.Nws tau tshaj tawm tias diludine tsis tsuas yog tuaj yeem txhawb kev loj hlob ntawm tsiaj, tab sis txhim kho kev ua haujlwm ntawm cov tsiaj txhu pom tseeb thiab txhim kho cev xeeb tub, tso zis ntawm mis nyuj, tso zis ntawm qe thiab hatching tus poj niam tsiaj.[1, 2, 5–7].Txoj kev tshawb fawb ntawm diludine hauv Suav teb tau pib los ntawm xyoo 1980s, thiab feem ntau ntawm cov kev tshawb fawb txog diludine hauv Suav teb tau txwv tsis pub siv cov txiaj ntsig txog tam sim no, thiab ob peb qhov kev sim ntawm kev tso cov noog tau tshaj tawm.Chen Jufang (1993) tau tshaj tawm tias diludine tuaj yeem txhim kho cov zis ntawm cov qe thiab qhov hnyav ntawm lub qe ntawm cov nqaij qaib, tab sis tsis tob zuj zus.[5]kev kawm ntawm lub mechanism ntawm kev txiav txim ntawm no.Yog li ntawd, peb tau siv txoj kev tshawb fawb txog cov txiaj ntsig thiab cov txheej txheem ntawm nws los ntawm kev pub mis rau nteg qe nrog cov zaub mov doped nrog diludine, thiab ib feem ntawm qhov tshwm sim tam sim no tau tshaj tawm raws li hauv qab no:
Table 1 Kev sib xyaw thiab cov khoom noj khoom haus ntawm kev sim noj zaub mov
%
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Muaj pes tsawg leeg ntawm cov khoom noj khoom haus Cheebtsam
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Pob kws 62 ME③ 11.97
Taum pulp 20 CP 17.8
Ntses noj 3 Ca 3.42
Rapeseed noj 5 P 0.75
Pob txha noj 2 M thiab 0.43
Pob zeb noj 7.5 M thiab Cys 0.75
Methionine 0.1
Ntsev 0.3
Multivitamin ① 10
Cov ntsiab lus ② 0.1
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① Multivitamin: 11mg ntawm riboflavin, 26mg ntawm folic acid, 44mg ntawm oryzanin, 66mg ntawm niacin, 0.22mg ntawm biotin, 66mg ntawm B6, 17.6ug ntawm B12, 880mg ntawm choline, 306mg ntawm IUK,E, 6600 ICU of VDthiab 20000ICU ntawm VA, yog ntxiv rau txhua kilogram ntawm cov khoom noj;thiab 10g multivitamin ntxiv rau txhua 50kg ntawm kev noj haus.
② Cov kab mob (mg / kg): 60 mg ntawm Mn, 60 mg ntawm Zn, 80mg ntawm Fe, 10mg ntawm Cu, 0.35mg ntawm kuv thiab 0.3mg ntawm Se ntxiv rau txhua kilogram ntawm cov khoom noj.
③ Chav tsev ntawm lub zog metabolizable hais txog MJ / kg.
1. Cov ntaub ntawv thiab txoj kev
1.1 Cov khoom siv kuaj
Beijing Sunpu Biochem.& Tech.Co., Ltd. yuav tsum muab cov diludine;thiab cov tsiaj sim yuav tsum xa mus rau Roman kev lag luam nteg qe uas muaj hnub nyoog 300 hnub.
Kev sim noj zaub mov: kev sim kev sim noj zaub mov yuav tsum tau npaj raws li qhov tshwm sim tiag tiag thaum lub sijhawm tsim khoom raws li tus qauv NRC, raws li qhia hauv Table 1.
1.2 Test method
1.2.1 Kev sim pub mis: kev sim pub mis yuav tsum tau ua hauv kev ua liaj ua teb ntawm Hongji Company hauv Jiande City;1024 Roman nteg hens yuav tsum raug xaiv thiab muab faib ua plaub pawg random thiab txhua rau 256 daim (txhua pawg yuav tsum rov ua plaub zaug, thiab txhua tus hen yuav tsum rov ua dua 64 zaug);cov hens yuav tsum tau noj nrog plaub cov khoom noj uas muaj cov ntsiab lus sib txawv ntawm diludine, thiab 0, 100, 150, 200mg / kg ntawm cov khoom noj yuav tsum tau ntxiv rau txhua pab pawg.Qhov kev xeem tau pib rau lub Plaub Hlis 10, 1997;thiab cov hens tuaj yeem nrhiav zaub mov thiab nqa dej dawb.Cov zaub mov noj los ntawm txhua pab pawg, tus nqi tso, qhov tso zis ntawm qe, lub qe tawg thiab cov qe txawv txav yuav tsum tau sau tseg.Tsis tas li ntawd, qhov kev xeem tau xaus rau lub Rau Hli 30, 1997.
1.2.2 Kev ntsuas lub qe zoo: 20 lub qe yuav tsum tau muab rho tawm thaum qhov kev ntsuam xyuas tau ua tiav plaub 40 hnub txhawm rau ntsuas cov ntsuas muaj feem xyuam rau lub qe zoo, xws li lub qe zoo li qhov ntsuas, lub hau hau, qhov hnyav ntawm lub plhaub, plhaub thickness, lub yolk index, tus txheeb ze qhov hnyav ntawm yolk, thiab lwm yam. Ntxiv mus, cov ntsiab lus ntawm cov roj cholesterol nyob rau hauv lub yolk yuav tsum tau ntsuas los ntawm kev siv COD-PAP txoj kev nyob rau hauv lub xub ntiag ntawm Cicheng reagent tsim los ntawm Ningbo Cixi Biochemical Test Plant.
1.2.3 Kev ntsuas cov ntshav biochemical index: 16 xeem hens yuav tsum tau muab coj los ntawm txhua pab pawg txhua lub sijhawm thaum qhov kev sim tau siv rau 30 hnub thiab thaum qhov kev xeem tiav los npaj cov ntshav tom qab kuaj ntshav los ntawm cov leeg ntawm tis.Cov ntshav yuav tsum tau khaws cia ntawm qhov kub thiab txias (-20 ℃) txhawm rau ntsuas qhov ntsuas biochemical cuam tshuam.Qhov feem pua ntawm cov rog hauv plab thiab daim siab lipid cov ntsiab lus yuav tsum tau ntsuas tom qab tua thiab tshem tawm cov rog hauv plab thiab lub siab thaum ua tiav cov ntshav kuaj.
Lub superoxide dismutase (SOD) yuav tsum tau ntsuas los ntawm kev siv txoj kev saturation nyob rau hauv lub xub ntiag ntawm cov khoom siv reagent tsim los ntawm Beijing Huaqing Biochem.& Tech.Lub koom haum tshawb fawb.Cov uric acid (UN) hauv cov ntshav yuav tsum ntsuas los ntawm kev siv U ricase-PAP txoj kev nyob rau hauv lub xub ntiag ntawm cov khoom siv Cicheng reagent;cov triglyceride (TG3) yuav tsum tau ntsuas los ntawm kev siv GPO-PAP ib-kauj ruam txoj kev nyob rau hauv lub xub ntiag ntawm cov khoom siv Cicheng reagent;lipase yuav tsum tau ntsuas los ntawm kev siv cov nephelometry nyob rau hauv lub xub ntiag ntawm cov khoom siv Cicheng reagent;Cov roj (cholesterol) tag nrho (CHL) yuav tsum ntsuas los ntawm kev siv COD-PAP txoj hauv kev muaj cov khoom siv Cicheng reagent;cov glutamic-pyruvic transaminase (GPT) yuav tsum tau ntsuas los ntawm kev siv cov colorimetry nyob rau hauv lub xub ntiag ntawm cov khoom siv Cicheng reagent;cov glutamic-oxalacetic transaminase (GOT) yuav tsum tau ntsuas los ntawm kev siv cov colorimetry nyob rau hauv lub xub ntiag ntawm cov khoom siv Cicheng reagent;alkaline phosphatase (ALP) yuav tsum tau ntsuas los ntawm kev siv tus nqi ntawm cov khoom siv Cicheng reagent;calcium ion (Ca2+) nyob rau hauv cov ntshav yuav tsum tau ntsuas los ntawm kev siv methylthymol xiav complexone txoj kev nyob rau hauv lub xub ntiag ntawm cov khoom siv Cicheng reagent;cov inorganic phosphorus (P) yuav tsum tau ntsuas los ntawm kev siv lub molybdate xiav txoj kev nyob rau hauv lub xub ntiag ntawm cov khoom siv Cicheng reagent.
2 Kev xeem tau
2.1 Qhov cuam tshuam rau kev tso tawm kev ua haujlwm
Cov kev ua yeeb yam ntawm cov pab pawg sib txawv ua tiav los ntawm kev siv diludine yog qhia hauv Table 2.
Table 2 Kev ua tau zoo ntawm hens noj nrog basal noj zaub mov ntxiv nrog plaub theem ntawm diludine
| Tus nqi ntawm diludine yuav tsum tau ntxiv (mg / kg) | ||||
| 0 | 100 | 150 | 200 | |
| Kev noj zaub mov kom tsawg (g) |  | |||
| Tus nqi (%) | ||||
| Qhov hnyav nruab nrab ntawm qe (g) | ||||
| Qhov piv ntawm cov khoom rau qe | ||||
| Lub qe tawg (%) | ||||
| Tus nqi ntawm qe txawv txav (%) | ||||
Los ntawm cov lus 2, qhov tso cov nqi ntawm txhua pab pawg ua tiav los ntawm kev siv diludine tau txhim kho pom tseeb, qhov txiaj ntsig thaum ua tiav los ntawm kev siv 150mg / kg yog qhov zoo tshaj plaws (txog 83.36%), thiab 11.03% (p<0.01) tau zoo dua piv rau. nrog pab pawg neeg siv;yog li ntawd cov diludine muaj cov nyhuv ntawm kev txhim kho tus nqi tso.Pom los ntawm qhov hnyav nruab nrab ntawm lub qe, qhov hnyav ntawm lub qe yog nce (p> 0.05) nrog rau kev nce diludine hauv cov khoom noj txhua hnub.Piv nrog rau pawg neeg siv, qhov sib txawv ntawm tag nrho cov txheej txheem ntawm cov pab pawg ua tiav los ntawm kev siv 200mg / kg ntawm diludine tsis pom tseeb thaum 1.79g ntawm kev noj zaub mov tau ntxiv qhov nruab nrab;Txawm li cas los xij, qhov sib txawv ua kom pom tseeb ntxiv nrog rau qhov nce diludine, thiab qhov sib txawv ntawm qhov sib piv ntawm cov khoom siv rau lub qe ntawm cov khoom ua tiav yog pom tseeb (p <0.05), thiab cov nyhuv yog qhov zoo tshaj plaws thaum 150mg / kg ntawm diludine thiab yog 1.25: 1 uas yog txo rau 10.36% (p <0.01) piv nrog rau pawg neeg siv.Pom los ntawm cov qe tawg ntawm txhua qhov ua tiav, cov qe tawg (p <0.05) tuaj yeem txo qis thaum cov diludine ntxiv rau cov khoom noj txhua hnub;thiab feem pua ntawm cov qe txawv txav tau txo qis (p <0.05) nrog rau kev nce diludine.
2.2 cuam tshuam rau qe zoo
Pom los ntawm Table 3, lub qe zoo Performance index thiab lub qe tshwj xeeb lub ntiajteb txawj nqus tsis cuam tshuam (p> 0.05) thaum cov diludine ntxiv rau cov khoom noj txhua hnub, thiab qhov hnyav ntawm lub plhaub yog nce ntxiv nrog rau cov diludine ntxiv rau cov khoom noj txhua hnub, nyob rau hauv qhov hnyav ntawm lub plhaub tau nce rau 10.58% thiab 10.85% (p <0.05) raws li piv nrog cov pab pawg siv thaum 150 thiab 200mg / kg ntawm diludine ntxiv;Lub thickness ntawm lub qe plhaub yog nce raws li diludine nyob rau hauv cov khoom noj txhua hnub, nyob rau hauv lub thickness ntawm lub qe plhaub yog nce rau 13.89% (p <0.05) thaum 100mg / kg ntawm diludine yog ntxiv piv nrog rau cov pab pawg neeg, thiab cov thicknesses. ntawm cov qe qe yog nce rau 19.44% (p <0.01) thiab 27.7% (p <0.01) raws li thaum ntxiv 150 thiab 200mg / kg.Lub Haugh chav tsev (p <0.01) tau txhim kho pom tseeb thaum lub diludine ntxiv, uas qhia tau hais tias diludine muaj cov txiaj ntsig ntawm kev txhawb nqa kev sib txuas ntawm tuab albumen ntawm qe dawb.Lub diludine muaj kev ua haujlwm ntawm kev txhim kho qhov Performance index ntawm yolk, tab sis qhov sib txawv tsis pom tseeb (p <0.05).Cov ntsiab lus ntawm cov roj cholesterol ntawm cov qe qe ntawm txhua pawg yog qhov sib txawv thiab tuaj yeem txo qis (p <0.05) tom qab ntxiv 150 thiab 200mg / kg ntawm diludine.Tus txheeb ze qhov hnyav ntawm lub qe qe sib txawv ntawm ib leeg vim qhov sib txawv ntawm diludine ntxiv, qhov txheeb ze qhov hnyav ntawm lub qe qe raug txhim kho rau 18.01% thiab 14.92% (p <0.05) thaum 150mg / kg thiab 200mg / kg piv. nrog pab pawg neeg siv;yog li ntawd, qhov tsim nyog diludine muaj cov nyhuv ntawm kev txhawb nqa synthesis ntawm qe qe.
Table 3 Cov teebmeem ntawm diludine rau qe zoo
| Tus nqi ntawm diludine yuav tsum tau ntxiv (mg / kg) | ||||
| Qe zoo | 0 | 100 | 150 | 200 |
| Qe zoo Performance index (%) |  | |||
| Lub ntiajteb txawj nqus (g / cm3) | ||||
| Kwv yees qhov hnyav ntawm qe plhaub (%) | ||||
| Thickness ntawm lub qe (mm) | ||||
| Hoob unit (U) | ||||
| Qe yolk index (%) | ||||
| Cov roj (cholesterol) siab (%) | ||||
| Qhov hnyav ntawm qe qe (%) | ||||
2.3 Qhov cuam tshuam rau feem pua ntawm cov rog hauv plab thiab cov ntsiab lus ntawm daim siab rog ntawm cov nteg qe
Saib daim duab 1 thiab daim duab 2 rau cov teebmeem ntawm diludine rau cov rog plab feem pua thiab cov ntsiab lus ntawm daim siab rog ntawm cov nteg qe
Daim duab 1 Cov nyhuv ntawm diludine rau feem pua ntawm plab rog (PAF) ntawm cov nteg qe
| Feem pua ntawm cov rog plab | |
| Tus nqi ntawm diludine yuav tsum tau ntxiv |
Daim duab 2 Cov nyhuv ntawm diludine rau daim siab rog cov ntsiab lus (LF) ntawm nteg hens
| Lub siab rog | |
| Tus nqi ntawm diludine yuav tsum tau ntxiv |
Pom los ntawm daim duab 1, cov feem pua ntawm plab rog ntawm pawg xeem raug txo rau 8.3% thiab 12.11% (p <0.05) raws li thaum 100 thiab 150mg / kg ntawm diludine piv nrog rau pawg neeg siv, thiab feem pua ntawm plab rog yog txo. rau 33.49% (p <0.01) thaum 200mg / kg ntawm diludine ntxiv.Pom los ntawm daim duab 2, lub siab rog cov ntsiab lus (qhuav qhuav) ua tiav los ntawm 100, 150, 200mg / kg ntawm diludine feem raug txo rau 15.00% (p <0.05), 15.62% (p <0.05) thiab 27.7% (p< 0.01) piv nrog rau pawg neeg siv;Yog li ntawd, diludine muaj cov nyhuv ntawm kev txo qis feem pua ntawm plab rog thiab lub siab rog cov ntsiab lus ntawm nteg cov ntsiab lus pom tseeb, uas cov nyhuv yog pom zoo thaum 200mg / kg ntawm diludine ntxiv.
2.4 Cov nyhuv rau cov ntshav biochemical index
Pom los ntawm Table 4, qhov sib txawv ntawm cov khoom ua tiav nyob rau theem I (30d) ntawm SOD kev xeem tsis pom tseeb, thiab cov ntshav biochemical indexes ntawm txhua pab pawg uas cov diludine ntxiv hauv Phase II (80d) ntawm qhov kev sim siab dua. tshaj cov pab pawg neeg siv (p<0.05).Cov uric acid (p <0.05) hauv cov ntshav tuaj yeem txo qis thaum 150mg / kg thiab 200mg / kg ntawm diludine ntxiv;thaum cov nyhuv (p <0.05) muaj nyob rau thaum 100mg / kg ntawm diludine ntxiv nyob rau theem I. Lub diludine tuaj yeem txo cov triglyceride hauv cov ntshav, uas cov nyhuv yog qhov zoo tshaj plaws (p <0.01) hauv pab pawg thaum 150mg / kg ntawm diludine yog ntxiv rau Phase I, thiab yog qhov zoo tshaj plaws hauv pab pawg thaum 200mg / kg ntawm diludine ntxiv hauv Phase II.Tag nrho cov roj (cholesterol) hauv cov ntshav yog txo nrog kev nce diludine ntxiv rau cov khoom noj txhua hnub, tshwj xeeb tshaj yog cov ntsiab lus ntawm tag nrho cov roj cholesterol hauv cov ntshav tau txo qis rau 36.36% (p<0.01) thiab 40.74% (p<0.01) raws li thaum 150mg / kg. thiab 200mg / kg ntawm diludine tau ntxiv hauv Phase I piv nrog rau pawg neeg siv, thiab txo rau 26.60% (p<0.01), 37.40% (p<0.01) thiab 46.66% (p <0.01) raws li thaum 100mg / kg, 150mg. / kg thiab 200mg / kg ntawm diludine yog ntxiv nyob rau theem II piv nrog rau pawg neeg siv.Ntxiv mus, ALP tau nce ntxiv raws li diludine ntxiv rau cov khoom noj txhua hnub, thaum qhov txiaj ntsig ntawm ALP hauv pab pawg uas 150mg / kg thiab 200mg / kg ntawm diludine ntxiv yog siab dua li pawg neeg siv (p<0.05) pom tseeb.
Table 4 Cov teebmeem ntawm diludine ntawm cov ntshav tsis muaj
| Tus nqi ntawm diludine yuav tsum tau ntxiv (mg / kg) hauv Phase I (30d) ntawm kev xeem | ||||
| Yam khoom | 0 | 100 | 150 | 200 |
| Superoxide dismutase (mg / ml) |  | |||
| Uric acid | ||||
| Triglyceride (mmol / L) | ||||
| Lipase (U / L) | ||||
| Cov roj (cholesterol) (mg / dL) | ||||
| Glutamic-pyruvic transaminase (U/L) | ||||
| Glutamic-oxalacetic transaminase (U/L) | ||||
| Alkaline phosphatase (mmol / L) | ||||
| Calcium ion (mmol / L) | ||||
| Inorganic phosphorus (mg / dL) | ||||
| Tus nqi ntawm diludine ntxiv (mg / kg) hauv Phase II (80d) ntawm kev xeem | ||||
| Yam khoom | 0 | 100 | 150 | 200 |
| Superoxide dismutase (mg / ml) |  | |||
| Uric acid | ||||
| Triglyceride (mmol / L) | ||||
| Lipase (U / L) | ||||
| Cov roj (cholesterol) (mg / dL) | ||||
| Glutamic-pyruvic transaminase (U/L) | ||||
| Glutamic-oxalacetic transaminase (U/L) | ||||
| Alkaline phosphatase (mmol / L) | ||||
| Calcium ion (mmol / L) | ||||
| Inorganic phosphorus (mg / dL) | ||||
3 Kev tshuaj xyuas thiab kev sib tham
3.1 Lub diludine hauv qhov kev sim txhim kho qhov tso tawm, qhov hnyav ntawm lub qe, lub Haugh chav tsev thiab qhov txheeb ze qhov hnyav ntawm lub qe qe, uas qhia tau hais tias diludine muaj kev cuam tshuam los ntawm kev txhawb nqa assimilation ntawm cov protein thiab txhim kho cov nyiaj synthesis ntawm tuab. albumen ntawm qe dawb thiab protein ntawm qe yolk.Tsis tas li ntawd, cov ntsiab lus ntawm uric acid hauv cov ntshav tau txo kom pom tseeb;thiab feem ntau nws tau lees paub tias qhov txo qis ntawm cov ntsiab lus ntawm cov tsis muaj protein ntau nitrogen hauv cov ntshav txhais tau hais tias cov catabolism ceev ntawm cov protein raug txo, thiab lub sijhawm khaws cia ntawm nitrogen raug ncua.Qhov txiaj ntsig no tau muab lub hauv paus ntawm kev khaws cov protein ntau ntxiv, txhawb kev tso qe thiab txhim kho qhov hnyav ntawm lub qe ntawm cov nteg qe.Cov txiaj ntsig ntawm qhov kev sim tau taw qhia tias cov nyhuv tso tawm yog qhov zoo tshaj plaws thaum 150mg / kg ntawm diludine tau ntxiv, uas yog qhov tseem ceeb raws li qhov tshwm sim.[6,7]ntawm Bao Erqing thiab Qin Shangzhi thiab tau los ntawm kev ntxiv diludine thaum lub sij hawm lig ntawm nteg qe .Cov nyhuv tau txo qis thaum tus nqi ntawm diludine ntau dua 150mg / kg, uas tej zaum yuav yog vim cov protein hloov pauv.[8]raug cuam tshuam vim ntau npaum li cas thiab ntau dhau ntawm cov metabolism hauv lub cev mus rau diludine.
3.2 Cov concentration ntawm Ca2+Nyob rau hauv cov ntshav ntawm cov qe tau txo qis, P hauv cov ntshav tau txo qis thaum pib thiab ALP kev ua haujlwm tau nce pom tseeb ntawm diludine, uas qhia tau tias diludine cuam tshuam cov metabolism ntawm Ca thiab P pom tseeb.Yue Wenbin qhia tias diludine tuaj yeem txhawb kev nqus[9] ntawm cov ntxhia pob zeb Fe thiab Zn;ALP feem ntau muaj nyob hauv cov ntaub so ntswg, xws li daim siab, pob txha, txoj hnyuv, lub raum, thiab lwm yam;ALP nyob rau hauv cov ntshav yog los ntawm daim siab thiab pob txha;ALP nyob rau hauv cov pob txha muaj nyob rau hauv osteoblast feem ntau thiab tuaj yeem sib txuas cov phosphate ion nrog Ca2 los ntawm cov ntshav tom qab kev hloov pauv los ntawm kev txhawb kev decomposition ntawm phosphate thiab nce cov concentration ntawm phosphate ion, thiab tau tso rau ntawm cov pob txha hauv daim ntawv ntawm hydroxyapatite, thiab lwm yam. Txhawm rau ua kom txo qis ntawm Ca thiab P hauv cov ntshav, uas zoo ib yam nrog kev nce ntawm lub qe plhaub tuab thiab qhov hnyav ntawm cov qe plhaub hauv cov qe ntsuas.Ntxiv mus, cov qe tawg thiab qhov feem pua ntawm cov qe txawv txav tau txo qis hauv cov ntsiab lus ntawm kev tso tawm, uas kuj piav qhia txog qhov no.
3.3 Lub plab rog deposition thiab lub siab rog cov ntsiab lus ntawm cov nteg qe tau txo kom pom tseeb los ntawm kev ntxiv diludine rau kev noj haus, uas qhia tau hais tias lub diludine muaj cov nyhuv ntawm inhibiting synthesis ntawm rog nyob rau hauv lub cev.Tsis tas li ntawd, diludine tuaj yeem txhim kho kev ua haujlwm ntawm lipase hauv cov ntshav hauv lub sijhawm ntxov;Qhov kev ua ntawm lipase tau nce pom tseeb hauv cov pab pawg uas 100mg / kg ntawm diludine tau ntxiv, thiab cov ntsiab lus ntawm triglyceride thiab cov roj cholesterol hauv cov ntshav tau txo qis (p <0.01), uas qhia tau tias diludine tuaj yeem txhawb kev decomposition ntawm triglyceride. thiab inhibit synthesis ntawm cov cholesterol.Cov rog rog tuaj yeem txwv tsis pub vim tias cov enzyme ntawm lipid metabolism hauv lub siab[10,11], thiab txo cov roj cholesterol hauv lub qe qe kuj piav qhia txog qhov no [13].Chen Jufang tau tshaj tawm tias diludine tuaj yeem txwv kev tsim cov rog hauv cov tsiaj thiab txhim kho cov nqaij ntshiv feem pua ntawm cov nqaij qaib thiab npua, thiab muaj cov txiaj ntsig ntawm kev kho lub siab rog.Cov txiaj ntsig ntawm qhov kev sim tau qhia meej txog qhov kev ua haujlwm no, thiab kev txiav tawm thiab kev soj ntsuam cov txiaj ntsig ntawm cov hens xeem kuj tau ua pov thawj tias diludine tuaj yeem txo qhov tshwm sim ntawm cov rog rog ntawm cov hens pom tseeb.
3.4 GPT thiab GOT yog ob qho tseem ceeb qhia txog kev ua haujlwm ntawm lub siab thiab lub plawv, thiab daim siab thiab lub plawv yuav raug puas tsuaj yog tias cov dej num ntawd siab dhau.Cov kev ua ub no ntawm GPT thiab GOT hauv cov ntshav tsis tau hloov pom tseeb thaum lub diludine ntxiv rau hauv qhov kev sim, uas qhia tau tias lub siab thiab lub plawv tsis raug puas tsuaj;Ntxiv mus, qhov kev ntsuas ntawm SOD tau pom tias kev ua haujlwm ntawm SOD hauv cov ntshav tuaj yeem txhim kho pom tseeb thaum cov diludine tau siv rau qee lub sijhawm.SOD yog hais txog cov khoom loj ntawm cov superoxide dawb radical hauv lub cev;Nws yog ib qho tseem ceeb rau kev tswj hwm kev ncaj ncees ntawm cov kab mob lom, txhim kho lub cev tiv thaiv kab mob thiab kev noj qab haus huv ntawm tus tsiaj thaum cov ntsiab lus ntawm SOD hauv lub cev nce.Quh Hai, thiab lwm yam tau tshaj tawm tias diludine tuaj yeem txhim kho cov haujlwm ntawm 6-glucose phosphate dehydrogenase nyob rau hauv cov ntaub so ntswg thiab stabilize cov ntaub so ntswg [2] ntawm cov kab mob lom.Sniedze tau taw qhia tias diludine txwv tsis pub ua haujlwm [4] ntawm NADPH cytochrome C reductase pom tseeb tom qab kawm txog kev sib raug zoo ntawm diludine thiab cov enzyme cuam tshuam hauv NADPH tshwj xeeb electron hloov saw hauv nas lub siab microsome.Odydents kuj tau taw qhia tias diludine muaj feem cuam tshuam [4] rau cov kab ke oxidase thiab microsomal enzyme ntsig txog NADPH;thiab cov txheej txheem ntawm kev ua ntawm diludine tom qab nkag mus rau hauv cov tsiaj yog ua lub luag haujlwm ntawm kev tiv thaiv oxidation thiab tiv thaiv cov kab mob lom neeg [8] los ntawm kev cuam tshuam cov kev ua ntawm electron hloov NADPH enzyme ntawm microsome thiab tswj cov txheej txheem peroxidation ntawm lipid compound.Cov txiaj ntsig kev xeem tau ua pov thawj tias kev tiv thaiv kev ua haujlwm ntawm diludine rau cov kab mob lom los ntawm kev hloov pauv ntawm SOD kev ua haujlwm rau kev hloov pauv ntawm GPT thiab GOT thiab ua pov thawj qhov kev tshawb fawb ntawm Sniedze thiab Odydents.
Kev siv
1 Zhou Kai, Zhou Mingjie, Qin Zhongzhi, thiab lwm yam. Kawm txog diludine ntawm kev txhim kho kev yug me nyuam ntawm yajJ. Nyaj thiabLkev ivestock 1994 (2): 16-17
2 Qu Hai, Lv Ye, Wang Baosheng, Cov nyhuv ntawm diludine ntxiv rau kev noj haus txhua hnub kom cev xeeb tub thiab cov phev zoo ntawm cov nqaij luav.J. Suav Journal of Rabbit Farming1994(6): 6-7
3 Chen Jufang, Yin Yuejin, Liu Wanhan, thiab lwm yam.Pub Kev Tshawb Fawb1993 (3): 2-4
4 Zheng Xiaozhong, Li Kelu, Yue Wenbin, lwm yam.Pub Kev Tshawb Fawb1995 (7): 12-13
5 Chen Jufang, Yin Yuejin, Liu Wanhan, thiab lwm yam.Pub Kev Tshawb Fawb1993 (3): 2-5
6 Bao Erqing, Gao Baohua, Kev sim ntawm diludine rau kev pub mis yug Peking osPub Kev Tshawb Fawb1992 (7): 7-8
7 Qin Shangzhi Kev ntsuam xyuas ntawm kev txhim kho cov khoom tsim ntawm cov nqaij qaib hauv lub sijhawm lig ntawm kev tso los ntawm kev siv diludineGuangxi Journal of Animal Husbandry & Veterinary Medicine1993.9(2): 26-27
8 Dibner J Jl Lvey FJ Hepatic protein thiab amino acid metabolian hauv nqaij qaib Nqaij qaib Sci1990.69(7): 1188-1194
9 Yue Wenbin, Zhang Jianhong, Zhao Peie, thiab lwm yam. Kawm txog kev ntxiv diludine thiab Fe-Zn npaj rau kev noj zaub mov txhua hnub ntawm nteg qe.Pub & Tsiaj1997, 18(7): 29-30
10 Mildner A na M, Steven D Clarke Porcine fatty acid synthase cloning ntawm ib qho ntxiv DNA, cov ntaub so ntswg faib ntawm itsmRNA thiab kev tawm tsam ntawm kev qhia los ntawm somatotropin thiab noj protein ntau J Nutri 1991, 121 900
11 W alzon RL Smon C, M orishita T, thiab I Fatty siab hemorrhagic syndrome nyob rau hauv hens overfed noj purified noj Xaiv enzyme kev ua ub no thiab daim siab histology nyob rau hauv relation mus rau daim siab Honorrhage thiab kev loj hlob kev ua tau zoo.Nqaij qaib Sci,1993 72(8): 1479-1491
12 Donaldson Peb Lipid metabolism hauv daim siab ntawm menyuam qaib teb rau kev pub misNqaij qaib Sci.1990, 69(7): 1183-1187
13 Ksiazk ieu icz J. K ontecka H, H ogcw sk i L A note on blood cholesterol as an indicator of body fatness in ducksPhau ntawv Journal of Aninal thiab Feed Science,1992, 1(3/4): 289-294
Post lub sij hawm: Jun-07-2021