NdemangaKuyesera kunachitika kuphunzira zotsatira za diludine pa kugona ntchito ndi khalidwe dzira mu nkhuku ndi njira ya limagwirira zotsatira ndi kudziwa zolozera dzira ndi seramu magawo 1024 ROM nkhuku anawagawa m'magulu anayi aliyense amene m'gulu replicates anayi 64 nkhuku aliyense, The mankhwala magulu analandira chimodzimodzi woyambira zakudya kuonjezera 0, 100, 150, 200 mg/kg diludine motero kwa 80 d.Zotsatira zake zinali motere.Kuwonjezera diludine kwa zakudya bwino nkhuku atagona ntchito, amene 150 mg/kg mankhwala anali bwino;mlingo wake wogona unawonjezeka ndi 11.8% (p<0.01), kutembenuka kwa dzira kunachepetsedwa ndi 10.36% (p<0 01).Kulemera kwa mazira kunawonjezeka ndi kuwonjezeka kwa diludine anawonjezera.Diludine idachepetsa kwambiri kuchuluka kwa uric acid mu seramu (p<0.01);kuwonjezera diludine kunachepetsa kwambiri seramu Ca²⁺ndi inorganic phosphate zili, ndi kuchuluka kwa zochita za alkine phosphatase (ALP) wa seramu (p<0.05), kotero izo zinali ndi zotsatira kwambiri kuchepetsa kusweka dzira (p<0.05) ndi abnormality (p <0.05);diludine kwambiri anawonjezera albumen kutalika.Mtengo wa Haugh (p <0.01), makulidwe a chipolopolo ndi kulemera kwa chipolopolo (p<0.05), 150 ndi 200mg/kg diludine adachepetsanso cholesterol mu dzira yolk (p<0 05), koma kuchuluka kwa dzira yolk kulemera (p <0.05).Kuphatikiza apo, diludine imatha kupititsa patsogolo ntchito ya lipase (p <0.01), ndikuchepetsa zomwe zili mu triglyceride (TG3) (p <0.01) ndi cholesterol (CHL) (p<0 01) mu seramu, zimachepetsa kuchuluka kwamafuta am'mimba. (p<0.01) ndi mafuta a chiwindi (p<0.01), anali ndi mphamvu zoletsa nkhuku ku chiwindi chamafuta.Diludine idachulukitsa kwambiri ntchito ya SOD mu seramu (p<0 01) pomwe idawonjezeredwa ku zakudya zopitilira 30d.Komabe, palibe kusiyana kwakukulu komwe kunapezeka muzochitika za GPT ndi GOT za seramu pakati pa gulu lolamulira ndi lothandizidwa.Zinkadziwika kuti diludine imatha kuteteza nembanemba ya ma cell kuti isakhale ndi okosijeni

Mawu ofunikaDiludine;nkhuku;SOD;cholesterol;triglycerides, lipase

 

Diludine ndi buku losapatsa thanzi la anti-oxidation vitamin additive ndipo limakhala ndi zotsatira zake.^[1-3]Kuletsa makutidwe ndi okosijeni a nembanemba ndi kukhazikika kwa maselo achilengedwe, ndi zina zambiri. M'zaka za m'ma 1970, katswiri wa zaulimi ku Latvia yemwe kale anali Soviet Union adapeza kuti diludine inali ndi zotsatirapo zake.^[4]kulimbikitsa kukula kwa nkhuku ndi kukana kuzizira ndi kukalamba kwa zomera zina.Zinanenedwa kuti diludine osati akhoza kulimbikitsa kukula kwa nyama, koma kusintha uchembere ntchito nyama mwachionekere ndi bwino mimba mlingo, linanena bungwe mkaka, linanena bungwe dzira ndi hatching mlingo wa mkazi nyama.^{[ 1, 2, 5-7 ]}.Kafukufuku wa diludine ku China adayambitsidwa kuyambira 1980s, ndipo maphunziro ambiri okhudza diludine ku China amangogwiritsa ntchito mpaka pano, ndipo mayeso ochepa okhudza mbalame zogonera adanenedwa.Chen Jufang (1993) adanena kuti diludine ikhoza kupititsa patsogolo kutulutsa kwa dzira ndi kulemera kwa dzira la nkhuku, koma silinazama.^[5]kuphunzira limagwirira ntchito zake.Chifukwa chake, tidagwiritsa ntchito kafukufuku watsatanetsatane wa momwe zimakhalira ndi momwe zimagwirira ntchito podyetsa nkhuku zogonera ndi chakudya chopangidwa ndi diludine, ndipo gawo limodzi lazotsatira likunena motere:

Table 1 Mapangidwe ndi zigawo zazakudya zoyesera

%

------------------------------------------------- -------------------------------------------

Kapangidwe ka zakudya Zopatsa thanzi zigawo

------------------------------------------------- -------------------------------------------

Chimanga 62 ME③ 11.97

Nyemba zamkati 20 CP 17.8

Zakudya za nsomba 3 Ca 3.42

Chakudya chogwiriridwa 5 P 0.75

Kudya kwa mafupa 2 M et 0.43

Chakudya chamwala 7.5 M et Cys 0.75

Methionine 0.1

Mchere 0.3

Multivitamin ① 10

Tsatirani zinthu② 0.1

------------------------------------------------- ----------------------------------------

① Multivitamin: 11mg wa riboflavin, 26mg wa folic acid, 44mg wa oryzanin, 66mg wa niacin, 0.22mg wa biotin, 66mg wa B6, 17.6ug wa B12, 880mg wa choline, 30mg wa VK, 66IU_E, 6600ICU ya V_Dndi 20000ICU ya V_A, amawonjezeredwa ku kilogalamu iliyonse ya zakudya;ndi 10g multivitamin anawonjezera aliyense 50kg zakudya.

② Tsatirani zinthu (mg/kg): 60 mg wa Mn, 60mg wa Zn, 80mg wa Fe, 10mg wa Cu, 0.35mg wa I ndi 0.3mg wa Se amawonjezeredwa pa kilogalamu iliyonse yazakudya.

③ Chigawo cha mphamvu zowonongeka chimatanthawuza MJ / kg.

 

1. Zida ndi njira

1.1 Zoyeserera

Beijing Sunpu Biochem.& Tech.Co., Ltd. ayenera kupereka diludine;ndipo nyama yoyesedwa idzatanthauza nkhuku zoikira malonda zachiroma zomwe zili ndi masiku 300.

 

Zakudya zoyesera: zakudya zoyeserera zoyeserera ziyenera kukonzedwa molingana ndi momwe zilili panthawi yopanga pamaziko a muyezo wa NRC, monga zikuwonetsedwa mu Gulu 1.

1.2 Njira yoyesera

1.2.1 Kuyesa kudyetsa: kuyesa kodyetsa kuyenera kuchitika pafamu ya Hongji Company ku Jiande City;1024 Nkhuku zoikira za Aroma ziyenera kusankhidwa ndikugawidwa m'magulu anayi mwachisawawa ndipo aliyense pazidutswa 256 (gulu lililonse libwereze kanayi, ndipo nkhuku iliyonse ibwerezedwe ka 64);Nkhuku ziyenera kudyetsedwa ndi zakudya zinayi zomwe zili ndi diludine, ndipo 0, 100, 150, 200mg/kg yazakudya ziyenera kuwonjezeredwa pagulu lililonse.Chiyesocho chinayambika pa April 10, 1997;ndipo nkhuku zinkakhoza kupeza chakudya ndi kumwa madzi kwaulere.Chakudya chotengedwa ndi gulu lirilonse, kuchuluka kwa kuikira, kutuluka kwa dzira, dzira losweka ndi chiwerengero cha dzira losazolowereka ziyenera kulembedwa.Ndiponso, mayesowo anatha pa June 30, 1997.

1.2.2 Kuyeza kwa dzira: Mazira 20 amayenera kumwedwa mwachisawawa pamene kuyezetsa kunachitika kwa masiku 40 kuti athe kuyeza zizindikiro zokhudzana ndi khalidwe la dzira, monga chiwerengero cha dzira, haugh unit, kulemera kwa chipolopolo, makulidwe a chipolopolo, cholozera cha yolk, kulemera kwake kwa yolk, ndi zina zambiri. Komanso, zomwe zili mu cholesterol mu yolk ziyenera kuyesedwa pogwiritsa ntchito njira ya COD-PAP pamaso pa Cicheng reagent yopangidwa ndi Ningbo Cixi Biochemical Test Plant.

1.2.3 Kuyeza kwa seramu biochemical index: nkhuku zoyezera 16 ziyenera kutengedwa kuchokera ku gulu lirilonse nthawi iliyonse pamene kuyezetsa kunayesedwa kwa masiku 30 komanso pamene kuyezetsa kwatha kuti akonze seramu pambuyo poyesa magazi kuchokera mumtsempha wa phiko.Seramu iyenera kusungidwa pamalo otsika kwambiri (-20 ℃) ​​kuti athe kuyeza ma biochemical indexes.Kuchuluka kwamafuta am'mimba ndi kuchuluka kwa lipids m'chiwindi ziyenera kuyezedwa pambuyo popha ndikuchotsa mafuta am'mimba ndi chiwindi mukamaliza kuyesa magazi.

The superoxide dismutase (SOD) iyenera kuyezedwa pogwiritsa ntchito njira ya machulukitsidwe pamaso pa zida za reagent zopangidwa ndi Beijing Huaqing Biochem.& Tech.Research Institute.Uric acid (UN) mu seramu iyenera kuyezedwa pogwiritsa ntchito njira ya U ricase-PAP pamaso pa zida za Cicheng reagent;triglyceride (TG3) iyenera kuyezedwa pogwiritsa ntchito GPO-PAP njira imodzi yokha pamaso pa zida za Cicheng reagent;lipase iyenera kuyeza pogwiritsa ntchito nephelometry pamaso pa zida za Cicheng reagent;seramu yonse ya cholesterol (CHL) iyenera kuyezedwa pogwiritsa ntchito njira ya COD-PAP pamaso pa zida za Cicheng reagent;glutamic-pyruvic transaminase (GPT) iyenera kuyezedwa pogwiritsa ntchito colorimetry pamaso pa zida za Cicheng reagent;glutamic-oxalacetic transaminase (GOT) iyenera kuyezedwa pogwiritsa ntchito colorimetry pamaso pa zida za Cicheng reagent;alkaline phosphatase (ALP) iyenera kuyeza pogwiritsa ntchito njira ya mlingo pamaso pa zida za Cicheng reagent;calcium ion (Ca²⁺) mu seramu iyenera kuyezedwa pogwiritsa ntchito njira ya methylthymol blue complexone pamaso pa zida za Cicheng reagent;phosphorous wachilengedwe (P) ayenera kuyezedwa pogwiritsa ntchito njira ya buluu ya molybdate pamaso pa zida za Cicheng reagent.

 

2 Zotsatira za mayeso

2.1 Zotsatira pakuyika magwiridwe antchito

Kuyika kwa magulu osiyanasiyana omwe amagwiritsidwa ntchito pogwiritsa ntchito diludine akuwonetsedwa mu Table 2.

Table 2 Magwiridwe a nkhuku zodyetsedwa ndi zakudya zoyambira zowonjezeredwa ndi milingo inayi ya diludine

 

Kuchuluka kwa diludine kuwonjezeredwa (mg/kg)
	0	100	150	200
Zakudya zamafuta (g)
Mtengo woyika (%)
Kulemera kwa dzira (g)
Chiŵerengero cha zinthu ndi dzira
Mazira osweka (%)
Mlingo wa dzira lachilendo (%)

 

Kuchokera patebulo 2, kuyika mitengo yamagulu onse okonzedwa pogwiritsa ntchito diludine kumakhala bwino mwachiwonekere, momwe zotsatira zake zikagwiritsidwa ntchito pogwiritsa ntchito 150mg/kg ndi zabwino kwambiri (mpaka 83.36%), ndi 11.03% (p<0.01) ndi bwino poyerekeza ndi gulu lolozera;chifukwa chake diludine imakhala ndi zotsatira zowongolera kuchuluka kwa kugona.Kuyang'ana pa kulemera kwa dzira, kulemera kwa dzira kumawonjezeka (p> 0.05) pamodzi ndi kuwonjezeka kwa diludine muzakudya za tsiku ndi tsiku.Poyerekeza ndi gulu lofotokozera, kusiyana pakati pa magawo onse okonzedwa a magulu omwe amagwiritsidwa ntchito pogwiritsa ntchito 200mg / kg ya diludine sizowoneka bwino pamene 1.79g ya chakudya chodyera ikuwonjezeredwa pafupifupi;Komabe, kusiyana kumakhala koonekeratu pang'onopang'ono pamodzi ndi kuwonjezeka kwa diludine, ndipo kusiyana kwa chiŵerengero cha zinthu ndi dzira pakati pa zigawo zomwe zimakonzedwa ndizodziwikiratu (p <0.05), ndipo zotsatira zake zimakhala zabwino pamene 150mg/kg ya diludine ndi 1.25: 1 yomwe imachepetsedwa kwa 10.36% (p <0.01) poyerekeza ndi gulu lofotokozera.Kuwoneka kuchokera ku dzira losweka la zigawo zonse zokonzedwa, dzira losweka (p <0.05) likhoza kuchepetsedwa pamene diludine ikuwonjezeredwa ku zakudya za tsiku ndi tsiku;ndipo kuchuluka kwa mazira osadziwika bwino kumachepetsedwa (p <0.05) pamodzi ndi kuchuluka kwa diludine.

 

2.2 Momwe dzira lilili

Tawonani pa Table 3, dzira mawonekedwe index ndi dzira yeniyeni mphamvu yokoka si anakhudzidwa (p> 0,05) pamene diludine anawonjezedwa kwa chakudya tsiku ndi tsiku, ndi kulemera kwa chipolopolo ndi kuchuluka pamodzi kuwonjezeka diludine anawonjezera tsiku chakudya, momwe kulemera kwa zipolopolo kumachulukitsidwa kwa 10.58% ndi 10.85% (p <0.05) motsatana poyerekeza ndi magulu ofotokozera pamene 150 ndi 200mg / kg ya diludine ikuwonjezeredwa;makulidwe a chipolopolo cha dzira amachulukitsidwa limodzi ndi kuchuluka kwa diludine muzakudya zatsiku ndi tsiku, momwe makulidwe a chipolopolo cha dzira amachulukitsidwa ndi 13.89% (p<0.05) pomwe 100mg/kg ya diludine ikuwonjezedwa poyerekeza ndi magulu ofotokozera, ndi makulidwe ake. a zipolopolo za dzira amachulukitsidwa kwa 19.44% (p<0.01) ndi 27.7% (p<0.01) motsatira pamene 150 ndi 200mg/kg awonjezeredwa.Chigawo cha Haugh (p <0.01) chimakhala bwino mwachiwonekere pamene diludine imawonjezedwa, zomwe zimasonyeza kuti diludine ili ndi zotsatira zolimbikitsa kaphatikizidwe ka albumen wandiweyani wa dzira loyera.Diludine ali ndi ntchito yokonza index wa yolk, koma kusiyana si mwachiwonekere (p <0.05).Zomwe zili mu cholesterol ya dzira yolk yamagulu onse ndizosiyana ndipo zikhoza kuchepetsedwa (p<0.05) mutawonjezera 150 ndi 200mg/kg ya diludine.Kulemera kwachibale kwa yolk ya dzira kumakhala kosiyana chifukwa cha kuchuluka kwa diludine komwe kumawonjezera, komwe kulemera kwa dzira yolk kumakhala bwino kwa 18.01% ndi 14.92% (p<0.05) pamene 150mg/kg ndi 200mg/kg poyerekeza ndi gulu lolozera;Choncho, diludine yoyenera imakhala ndi zotsatira zolimbikitsa kaphatikizidwe ka dzira yolk.

 

Table 3 Zotsatira za diludine pa khalidwe la dzira

Kuchuluka kwa diludine kuwonjezeredwa (mg/kg)
Mazira abwino	0	100	150	200
Mlozera wa dzira (%)
Kukoka kwa dzira (g/cm3)
Kulemera kwake kwa chipolopolo cha dzira (%)
Makulidwe a chipolopolo cha dzira (mm)
Chigawo cha Haugh (U)
Mazira yolk index (%)
Cholesterol ya dzira yolk (%)
Kulemera kwake kwa dzira yolk (%)

 

2.3 Zotsatira za kuchuluka kwa mafuta m'mimba ndi zomwe zili m'chiwindi cha nkhuku zoikira

Onani Chithunzi 1 ndi Chithunzi 2 za zotsatira za diludine kumafuta am'mimba komanso zomwe zili m'chiwindi cha nkhuku zoikira.

 

 

 

Chithunzi 1 Zotsatira za diludine pa kuchuluka kwa mafuta am'mimba (PAF) a nkhuku zoikira

 

	Peresenti yamafuta am'mimba
	Kuchuluka kwa diludine kuwonjezeredwa

 

 

Chithunzi 2 Zotsatira za diludine pamafuta a chiwindi (LF) a nkhuku zoikira

	Mafuta a chiwindi
	Kuchuluka kwa diludine kuwonjezeredwa

Kuchokera pa Chithunzi 1, kuchuluka kwa mafuta am'mimba a gulu loyeserera kumachepetsedwa 8.3% ndi 12.11% (p<0.05) motsatana pamene 100 ndi 150mg/kg wa diludine poyerekeza ndi gulu lofotokozera, ndipo kuchuluka kwamafuta am'mimba kumachepetsedwa. kwa 33.49% (p<0.01) pamene 200mg/kg ya diludine yawonjezeredwa.Kuwoneka kuchokera ku Chithunzi 2, mafuta a chiwindi (ouma kotheratu) opangidwa ndi 100, 150, 200mg/kg ya diludine motsatana amachepetsedwa ndi 15.00% (p<0.05), 15.62% (p<0.05) ndi 27.7% (p< 0.01) motsatana poyerekeza ndi gulu lolozera;Choncho, diludine ali ndi zotsatira za kuchepetsa kuchuluka kwa m`mimba mafuta ndi chiwindi mafuta okhutira atagona zili mwachionekere, mmene zotsatira mulingo woyenera kwambiri pamene 200mg/kg wa diludine anawonjezera.

2.4 Zotsatira za seramu biochemical index

Tawonani pa Table 4, kusiyana pakati pa zigawo zomwe zakonzedwa pa Gawo I (30d) la mayeso a SOD sizodziwikiratu, ndipo seramu biochemical indexes yamagulu onse omwe diludine amawonjezedwa mu Gawo II (80d) la mayeso ndi apamwamba. kuposa gulu lolozera (p <0.05).Uric acid (p<0.05) mu seramu imatha kuchepetsedwa pamene 150mg/kg ndi 200mg/kg ya diludine yawonjezeredwa;pamene zotsatira (p<0.05) zilipo pamene 100mg/kg wa diludine wawonjezeredwa Phase I. The diludine akhoza kuchepetsa triglyceride mu seramu, mmene mulingo woyenera kwambiri (p<0.01) mu gulu pamene 150mg/kg wa diludine amawonjezedwa mu Gawo I, ndipo ali mulingo woyenera pagulu pamene 200mg/kg ya diludine yawonjezeredwa mu Gawo II.Okwana mafuta m`thupi mu seramu yafupika pamodzi kuwonjezeka diludine anawonjezera tsiku chakudya, makamaka nkhani za okwana mafuta m`thupi mu seramu yafupika kwa 36.36% (p<0.01) ndi 40,74% (p<0.01) motero pamene 150mg/kg. ndi 200mg/kg wa diludine akuwonjezedwa mu Phase I poyerekeza ndi zolozera gulu, ndi kuchepetsedwa kwa 26.60% (p<0.01), 37.40% (p<0.01) ndi 46.66% (p<0.01) motero pamene 100mg/kg, 150mg. / kg ndi 200mg/kg ya diludine amawonjezedwa mu Gawo II poyerekeza ndi gulu lolozera.Kuphatikiza apo, ALP imachulukitsidwa limodzi ndi kuchuluka kwa diludine kuwonjezeredwa ku zakudya zatsiku ndi tsiku, pomwe mikhalidwe ya ALP m'gulu lomwe 150mg/kg ndi 200mg/kg ya diludine imawonjezedwa ndipamwamba kuposa gulu lofotokozera (p<0.05) mwachiwonekere.

Table 4 Zotsatira za diludine pazigawo za seramu

Kuchuluka kwa diludine kuwonjezeredwa (mg/kg) mu Gawo I (30d) la mayeso
Kanthu	0	100	150	200
Superoxide dismutase (mg/mL)
Uric acid
Triglyceride (mmol/L)
Lipase (U/L)
Cholesterol (mg/dL)
Glutamic-pyruvic transaminase (U/L)
Glutamic-oxalacetic transaminase (U/L)
Alkaline phosphatase (mmol/L)
Calcium ion (mmol/L)
Phosphorous inorganic (mg/dL)

 

Kuchuluka kwa diludine kuwonjezeredwa (mg/kg) mu Gawo II (80d) la mayeso
Kanthu	0	100	150	200
Superoxide dismutase (mg/mL)
Uric acid
Triglyceride (mmol/L)
Lipase (U/L)
Cholesterol (mg/dL)
Glutamic-pyruvic transaminase (U/L)
Glutamic-oxalacetic transaminase (U/L)
Alkaline phosphatase (mmol/L)
Calcium ion (mmol/L)
Phosphorous inorganic (mg/dL)

 

3 Kusanthula ndi kukambirana

3.1 The diludine mu mayeso bwino atagona mlingo, kulemera kwa dzira, ndi Haugh unit ndi wachibale kulemera kwa dzira yolk, zomwe zinasonyeza kuti diludine anali ndi zotsatira za kulimbikitsa makonzedwe a mapuloteni ndi kuwongolera kuchuluka kwa kaphatikizidwe wandiweyani. albumen wa dzira loyera ndi mapuloteni a dzira yolk.Komanso, zomwe zili mu uric acid mu seramu zidachepetsedwa mwachiwonekere;ndipo zinavomerezedwa kuti kuchepetsa zomwe zili ndi nayitrogeni wopanda mapuloteni mu seramu kumatanthauza kuti liwiro la catabolism la puloteni linachepetsedwa, ndipo nthawi yosungira nayitrogeni idaimitsidwa.Chotsatirachi chinapereka maziko owonjezera kusunga mapuloteni, kulimbikitsa kuikira mazira ndi kuwongolera kulemera kwa dzira la nkhuku zoikira.Zotsatira za mayesowo zikuwonetsa kuti kuyika kwake kumakhala koyenera pamene 150mg/kg ya diludine idawonjezeredwa, zomwe zinali zogwirizana ndi zotsatira zake.^[6,7]a Bao Erqing ndi Qin Shangzhi ndipo adapezedwa powonjezera diludine kumapeto kwa nthawi yoikira nkhuku.Zotsatira zinachepa pamene kuchuluka kwa diludine kuposa 150mg/kg, amene mwina chifukwa mapuloteni kusintha.^[8]anakhudzidwa chifukwa kwambiri mlingo ndi kwambiri katundu wa kagayidwe wa limba kuti diludine.

3.2 Kukhazikika kwa Ca²⁺mu seramu ya kuikira dzira inachepetsedwa, P mu seramu idachepetsedwa pachiyambi ndipo ntchito ya ALP inawonjezeka mwachiwonekere pamaso pa diludine, zomwe zimasonyeza kuti diludine zimakhudza kagayidwe ka Ca ndi P mwachiwonekere.Yue Wenbin adanenanso kuti diludine imatha kulimbikitsa kuyamwa^[9] zinthu zamchere Fe ndi Zn;ALP makamaka analipo mu zimakhala, monga chiwindi, fupa, matumbo thirakiti, impso, etc.;ALP mu seramu anali kuchokera pachiwindi ndi fupa makamaka;ALP mu fupa analipo mu osteoblast makamaka ndipo ankatha kuphatikiza phosphate ayoni ndi Ca2 ku seramu pambuyo kusintha ndi kulimbikitsa kuwonongeka wa mankwala ndi kuonjezera ndende ya phosphate ayoni, ndipo waikamo pa fupa mu mawonekedwe a hydroxyapatite, etc. pofuna kuchepetsa Ca ndi P mu seramu, zomwe zimagwirizana ndi kuwonjezeka kwa chipolopolo cha dzira ndi kulemera kwake kwa chigoba cha dzira mu zizindikiro za khalidwe la dzira.Komanso, kuchuluka kwa dzira losweka ndi kuchuluka kwa dzira losazolowereka kunachepetsedwa mwachiwonekere ponena za kuyika kwa ntchito, zomwe zinafotokozeranso mfundoyi.

3.3 Kuyika kwamafuta am'mimba ndi mafuta a chiwindi a nkhuku zoikira kunachepetsedwa mwachiwonekere powonjezera diludine ku zakudya, zomwe zimasonyeza kuti diludine inali ndi zotsatira zoletsa kaphatikizidwe ka mafuta m'thupi.Komanso, diludine amatha kusintha ntchito ya lipase mu seramu koyambirira;ntchito ya lipase chinawonjezeka mwachionekere mu gulu limene 100mg/kg wa diludine anawonjezera, ndi nkhani za triglyceride ndi mafuta m`thupi mu seramu yafupika (p<0.01), zomwe zimasonyeza kuti diludine akhoza kulimbikitsa kuwonongeka kwa triglyceride. ndikuletsa kaphatikizidwe ka cholesterol.Kuyika kwamafuta kumatha kuletsedwa chifukwa cha enzyme ya lipid metabolism m'chiwindi<sup>[10, 11]</sup>, ndi kuchepetsa mafuta m`thupi mu dzira yolk anafotokozanso mfundo imeneyi [13].Chen Jufang adanenanso kuti diludine imatha kuletsa mapangidwe amafuta mu nyama ndikuwongolera kuchuluka kwa nyama yowonda ya broilers ndi nkhumba, komanso kumathandizira kuchiza chiwindi chamafuta.Zotsatira za mayeso anafotokoza limagwirira ntchito, ndi dissection ndi kuonerera zotsatira za mayeso nkhuku komanso anatsimikizira kuti diludine akhoza kuchepetsa zimachitika mlingo wa mafuta chiwindi cha atagona nkhuku mwachionekere.

3.4 GPT ndi GOT ndi zizindikiro ziwiri zofunika zomwe zikuwonetsera ntchito ya chiwindi ndi mtima, ndipo chiwindi ndi mtima zikhoza kuwonongeka ngati ntchito zake zili zokwera kwambiri.Ntchito za GPT ndi GOT mu seramu sizinasinthidwe mwachiwonekere pamene diludine imawonjezeredwa muyeso, zomwe zimasonyeza kuti chiwindi ndi mtima sizinawonongeke;kupitilira apo, zotsatira zoyezera za SOD zikuwonetsa kuti ntchito ya SOD mu seramu ikhoza kuwongolera mwachiwonekere pamene diludine idagwiritsidwa ntchito kwakanthawi.SOD imatanthawuza scavenger wamkulu wa superoxide free radical m'thupi;Ndikofunikira pakusunga umphumphu wa nembanemba yachilengedwe, kukonza chitetezo chathupi komanso kukhala ndi thanzi la chiweto pomwe zomwe zili mu SOD m'thupi ziwonjezedwa.Quh Hai, etc. inanena kuti diludine akhoza kusintha ntchito ya 6-shuga mankwala dehydrogenase mu zamoyo nembanemba ndi kukhazikika zimakhala [2] wa kwachilengedwenso selo.Sniedze adawonetsa kuti diludine idaletsa ntchito [4] ya NADPH cytochrome C reductase mwachiwonekere ataphunzira za ubale wa diludine ndi enzyme yofunikira mu NADPH yeniyeni yosinthira ma elekitironi mu microsome yachiwindi cha makoswe.Odydents adanenanso kuti diludine inali yokhudzana ndi [4] ku dongosolo la oxidase la composite ndi enzyme ya microsomal yokhudzana ndi NADPH;ndi limagwirira ntchito diludine atalowa nyama ndi kuchita mbali kukana makutidwe ndi okosijeni ndi kuteteza zamoyo nembanemba [8] mwa intercepting ntchito ya elekitironi kutengerapo NADPH puloteni wa microsome ndi kuletsa njira peroxidation wa lipid pawiri.Zotsatira za mayeso zinatsimikizira kuti ntchito ya chitetezo cha diludine ku nembanemba yachilengedwe kuchokera ku kusintha kwa SOD kupita ku kusintha kwa ntchito za GPT ndi GOT ndikutsimikizira zotsatira za phunziro la Sniedze ndi Odydents.

 

Buku

1 Zhou Kai, Zhou Mingjie, Qin Zhongzhi, ndi ena. Phunzirani za diludine pakuwongolera ubereki wa nkhosa.J. Grass ndiLivestock 1994 (2): 16-17

2 Qu Hai, Lv Ye, Wang Baosheng, Zotsatira za diludine zomwe zimawonjezeredwa ku zakudya zatsiku ndi tsiku ku mlingo wa mimba ndi khalidwe la umuna wa kalulu wa nyama.J. Chinese Journal ya Kulima Akalulu1994 (6): 6-7

3 Chen Jufang, Yin Yuejin, Liu Wanhan, etc. Mayeso owonjezera kugwiritsa ntchito diludine monga chowonjezera chakudyaKafukufuku Wodyetsa1993 (3): 2-4

4 Zheng Xiaozhong, Li Kelu, Yue Wenbin, etc. Kukambitsirana za ntchito zotsatira ndi limagwirira zochita za diludine monga nkhuku wolimbikitsa kukulaKafukufuku Wodyetsa1995 (7): 12-13

5 Chen Jufang, Yin Yuejin, Liu Wanhan, etc. Mayeso owonjezera kugwiritsa ntchito diludine monga chowonjezera cha chakudya.Kafukufuku Wodyetsa1993 (3): 2-5

6 Bao Erqing, Gao Baohua, Mayeso a diludine podyetsa bakha PekingKafukufuku Wodyetsa1992 (7): 7-8

7 Qin Shangzhi Mayeso opititsa patsogolo zokolola za nkhuku zanyama kumapeto kwa nthawi yogona pogwiritsa ntchito diludineGuangxi Journal of Animal Husbandry & Veterinary Medicine1993.9(2): 26-27

8 Dibner J Jl Lvey FJ ​​Mapuloteni a chiwindi ndi amino acid metabolian mu nkhuku Nkhuku Sci1990.69(7): 1188- 1194

9 Yue Wenbin, Zhang Jianhong, Zhao Peie, ndi zina. Phunziro la kuwonjezera diludine ndi Fe-Zn kukonzekera ku chakudya cha tsiku ndi tsiku cha nkhuku zoikira.Zakudya & Ziweto1997, 18(7): 29-30

10 Mildner A ndi M, Steven D Clarke Porcine fatty acid synthase cloning ya DNA yowonjezera, kugawa kwa minofu ya itsmRNA ndi kuponderezedwa kwa mawu ndi somatotropin ndi mapuloteni a zakudya J Nutri 1991, 121 900

11 W alzon RL Smon C, M orishita T, et a I Fatty chiwindi hemorrhagic syndrome mu nkhuku overfed chakudya choyeretsedwa Kusankhidwa kwa enzyme ndi chiwindi histology mogwirizana ndi chiwindi ulemu ndi ntchito yobereka.Nkhuku Sci,1993 72(8): 1479- 1491

12 Donaldson WE Lipid metabolism m'chiwindi cha anapiye poyankha kudyetsedwaNkhuku Sci.1990, 69(7) : 1183- 1187

13 Ksiazk ieu icz J. K ontecka H, ​​H ogcw sk i L Cholesterol m'magazi monga chizindikiro cha kunenepa kwambiri kwa abakha.Journal ya Aninal ndi Feed Science,1992, 1(3/4): 289-294