AbstractUcwaningo lwenziwa ukuze kucwaningwe imiphumela ye-diludine ekubekeni ukusebenza kanye nekhwalithi yeqanda ezikhukhukazini kanye nendlela yokwenza imiphumela ngokunquma izinkomba zemingcele yeqanda ne-serum 1024 ROM izikhukhukazi zahlukaniswa ngamaqembu amane ngalinye elihlanganisa iziphindaphinda ezine ze-64. izinkukhu ngayinye, Amaqembu okwelapha athole ukudla okufanayo okuyisisekelo okuhlanganiswe no-0, 100, 150, 200 mg/kg diludine ngokulandelana kwama-80 d.Imiphumela yaba kanje.Ukwengezwa kwe-diludine ekudleni kwathuthukisa ukusebenza kwezinkukhu ezizalelayo, lapho ukwelashwa okungu-150 mg/kg kwakungcono kakhulu;izinga layo lokulala lenyuke ngo-11.8% (p< 0.01), ukuguqulwa kwenqwaba yamaqanda kwehle ngo-10.36% (p< 0 01).Izisindo zamaqanda zanda ngokukhula kwe-diludine yengezwe.I-Diludine yehlise kakhulu ukugcwala kwe-uric acid ku-serum (p<0.01);ukwengeza i-diludine kwehlisa kakhulu i-serum Ca2+kanye nokuqukethwe kwe-phosphate ye-inorganic, kanye nomsebenzi owandisiwe we-alkine phosphatase (ALP) ye-serum (p<0.05), ngakho kwaba nemiphumela ebalulekile ekwehliseni ukuphuka kwamaqanda (p<0.05) nokungajwayelekile (p <0.05);i-diludine yandisa kakhulu ukuphakama kwe-albhamuen.Inani le-Haugh (p <0.01), ukujiya kwegobolondo kanye nesisindo segobolondo (p< 0.05), 150 kanye no-200mg/kg i-diludine nayo yehlise isamba se-choleasterol ku-yolk yeqanda (p< 0 05), kodwa yanda isisindo se-yolk yeqanda (p <0.05).Ngaphezu kwalokho, i-diludine ingathuthukisa umsebenzi we-lipase (p <0.01), futhi yehlise okuqukethwe kwe-triglyceride (TG3) (p<0.01) ne-cholesterol (CHL) (p<0 01) ku-serum, yehlisa iphesenti lamafutha esisu. (p<0.01) kanye nokuqukethwe kwamafutha esibindi (p<0.01), kwakunamandla okuvimbela izinkukhu esibindini esinamafutha.I-Diludine yandisa kakhulu umsebenzi we-SOD ku-serum (p<0 01) lapho yengezwa ekudleni isikhathi esingaphezu kwe-30d.Nokho, awukho umehluko obalulekile otholakele emisebenzini ye-GPT kanye ne-GOT ye-serum phakathi kweqembu elilawulayo neliphathwayo.Kwacatshangwa ukuthi i-diludine ingavimbela ulwelwesi lwamaseli kusuka ku-oxidation
Amagama ayisihluthuleloI-Diludine;isikhukhukazi;I-SOD;i-cholesterol;triglycerides, lipase
I-diludine iyinoveli engeyona i-nutritive anti-oxidation vitamin additive futhi inemiphumela[1-3]yokuvimbela i-oxidation yolwelwesi lwebhayoloji kanye nokuzinzisa izicubu zamangqamuzana ezinto eziphilayo, njll. Ngawo-1970, uchwepheshe wezolimo waseLatvia ezweni elaliyiSoviet Union wathola ukuthi i-diludine yayinemiphumela.[4]wokuthuthukisa ukukhula kwezinkukhu kanye nokumelana nokubanda nokuguga kwezinye izitshalo.Kwabikwa ukuthi i-diludine ayigcini nje ngokukhuthaza ukukhula kwesilwane, kodwa ithuthukise ukusebenza kokuzala kwesilwane ngokusobala futhi ithuthukise izinga lokukhulelwa, ukuphuma kobisi, ukuphuma kweqanda kanye nezinga lokuchanyuselwa kwesilwane sesifazane.[1, 2, 5-7].Ucwaningo lwe-diludine e-china lwaqalwa kusukela ngawo-1980, futhi iningi lezifundo mayelana ne-diludine eShayina lisebenzisa umphumela kuze kube manje, futhi izivivinyo ezimbalwa zokubekela izinyoni zabikwa.U-Chen Jufang (1993) ubike ukuthi i-diludine ingathuthukisa ukuphuma kweqanda nesisindo seqanda lezinkukhu, kodwa ayizange ijule.[5]isifundo sendlela yokusebenza kwayo.Ngakho-ke, senze ucwaningo oluhlelekile lomphumela nendlela yakho ngokuphakela izinkukhu ezizalelayo ngokudla okufakwe i-diludine, futhi ingxenye eyodwa yomphumela manje ibikwa kanje:
Ithebula 1 Ukwakheka kanye nezingxenye zemisoco yokudla okuhlolwayo
%
------------------------------------------------- -------------------------------------------
Ukwakheka kokudla Izakhi zomsoco
------------------------------------------------- -------------------------------------------
Ummbila 62 ME③ 11.97
I-pulp kabhontshisi 20 CP 17.8
Isidlo sezinhlanzi 3 Ca 3.42
Isidlo sokudlwengulwa 5 P 0.75
Ukudla kwamathambo 2 M et 0.43
Ukudla kwamatshe 7.5 M et Cys 0.75
I-Methionine 0.1
Usawoti 0.3
I-Multivitamin① 10
Landela izakhi② 0.1
------------------------------------------------- ----------------------------------------
① I-Multivitamin: 11mg ye-riboflavin, 26mg ye-folic acid, 44mg ye-oryzanin, 66mg ye-niacin, 0.22mg ye-biotin, 66mg ye-B6, 17.6ug ye-B12, 880mg ye-choline, 30mg ye-VK, 66IUE, 6600ICU ye-VDkanye ne-20000ICU ye-VA, zengezwe kilogram ngayinye yokudla;kanye ne-10g ye-multivitamin yengezwe ku-50kg ngayinye yokudla.
② Izakhi zokulandelela (mg/kg): 60 mg we-Mn, 60mg we-Zn, 80mg we-Fe, 10mg we-Cu, 0.35mg we-I kanye no-0.3mg we-Se wengezwa kukhilogremu ngayinye yokudla.
③ Iyunithi yamandla agaywayo isho i-MJ/kg.
1. Izinto kanye nendlela
1.1 Izinto zokuhlola
I-Beijing Sunpu Biochem.& Ubuchwepheshe.I-Co., Ltd. kufanele inikeze i-diludine;futhi isilwane esivivinyayo sizobhekisela ezinkukhu zamazalela zaseRoma ezinezinsuku ezingama-300 ubudala.
Ukudla okuhlolwayo: ukudla okuhlolwayo kufanele kulungiswe ngokuya ngesimo sangempela ngesikhathi sokukhiqiza ngesisekelo sezinga le-NRC, njengoba kukhonjisiwe kuThebula 1.
1.2 Indlela yokuhlola
1.2.1 Ukuhlolwa kokuphakelayo: ukuhlolwa kokuphakela kufanele kwenziwe epulazini leNkampani yaseHongji eDolobheni laseJiande;1024 Izikhukhukazi zamaRoma ezizalelayo kufanele zikhethwe futhi zihlukaniswe ngamaqembu amane ngokungahleliwe futhi ngalinye libe yizicucu ezingu-256 (iqembu ngalinye kufanele liphindwe izikhathi ezine, futhi inkukhu ngayinye kufanele iphindwe izikhathi ezingu-64);izikhukhukazi kufanele zidliwe ngezidlo ezine ezinokuqukethwe okuhlukene kwe-diludine, futhi u-0, 100, 150, 200mg/kg wokudla kufanele kwengezwe eqenjini ngalinye.Ukuhlolwa kwaqalwa ngo-April 10, 1997;nezikhukhukazi zazingathola ukudla futhi zithathe amanzi mahhala.Ukudla okuthathwe yiqembu ngalinye, izinga lokuzala, ukuphuma kweqanda, iqanda eliphukile kanye nenani leqanda elingavamile kufanele kurekhodwe.Ngaphezu kwalokho, ukuhlolwa kwaqedwa ngo-June 30, 1997.
1.2.2 Ukulinganisa ikhwalithi yamaqanda: Amaqanda angama-20 kufanele athathwe ngokungahleliwe lapho ukuhlolwa kwenziwa izinsuku ezine ezingu-40 ukuze kulinganiswe izinkomba ezihlobene nekhwalithi yeqanda, njenge-egg shape index, i-haugh unit, isisindo esilinganiselwe segobolondo, ukujiya kwegobolondo, inkomba yesikhupha, isisindo esilinganiselwe se-yolk, njll. Ngaphezu kwalokho, okuqukethwe kwe-cholesterol ku-yolk kufanele kukalwe kusetshenziswa indlela ye-COD-PAP kukhona i-Cicheng reagent ekhiqizwa yi-Ningbo Cixi Biochemical Test Plant.
1.2.3 Ukukalwa kwe-serum biochemical index: Izikhukhukazi zokuhlola eziyi-16 kufanele zithathwe eqenjini ngalinye isikhathi ngasinye lapho ukuhlolwa kwenziwa izinsuku ezingama-30 nalapho ukuhlolwa sekuqediwe ukuze kulungiswe i-serum ngemva kokuthatha isampula yegazi emthanjeni ophikweni.I-serum kufanele igcinwe ezingeni lokushisa eliphansi (-20 ℃) ukuze kulinganiswe izinkomba ezifanele ze-biochemical.Iphesenti lamafutha esisu kanye ne-lipid yesibindi kufanele kulinganiswe ngemva kokuhlatshwa nokukhipha amanoni esisu kanye nesibindi lapho kuqedwa ukuthatha amasampula egazi.
I-superoxide dismutase (SOD) kufanele ikalwe kusetshenziswa indlela yokugcwala kwamanzi phambi kwekhithi ye-reagent ekhiqizwe yi-Beijing Huaqing Biochem.& Ubuchwepheshe.Isikhungo Socwaningo.I-uric acid (UN) ku-serum kufanele ikalwe ngokusebenzisa indlela ye-U ricase-PAP phambi kwekhithi ye-Cicheng reagent;i-triglyceride (TG3) kufanele ikalwe ngokusebenzisa indlela yesinyathelo esisodwa ye-GPO-PAP phambi kwekhithi ye-Cicheng reagent;i-lipase kufanele ilinganiswe ngokusebenzisa i-nephelometry phambi kwekhithi ye-Cicheng reagent;i-serum total cholesterol (CHL) kufanele ikalwe ngokusebenzisa indlela ye-COD-PAP phambi kwekhithi ye-Cicheng reagent;i-glutamic-pyruvic transaminase (GPT) kufanele ikalwe ngokusebenzisa i-colorimetry phambi kwekhithi ye-Cicheng reagent;i-glutamic-oxalacetic transaminase (GOT) kufanele ikalwe ngokusebenzisa i-colorimetry phambi kwekhithi ye-Cicheng reagent;i-alkaline phosphatase (ALP) kufanele ikalwe ngokusebenzisa indlela yesilinganiso phambi kwekhithi ye-Cicheng reagent;i-calcium ion (Ca2+) ku-serum kufanele kulinganiswe ngokusebenzisa indlela ye-methylthymol blue complexone phambi kwekhithi ye-Cicheng reagent;i-phosphorus ye-inorganic (P) kufanele ikalwe ngokusebenzisa indlela eluhlaza okwesibhakabhaka ye-molybdate phambi kwekhithi ye-Cicheng reagent.
2 Umphumela wokuhlolwa
2.1 Umthelela ekusebenzeni kokubeka
Imisebenzi yokubeka yamaqembu ahlukene asetshenzwe kusetshenziswa i-diludine ikhonjiswe kuThebula lesi-2.
Ithebula 2 Ukusebenza kwezikhukhukazi ezidliwe nge-basal diet ehambisana namazinga amane e-diludine
| Inani le-diludine elizongezwa (mg/kg) | ||||
| 0 | 100 | 150 | 200 | |
| Ukudla okuphakelayo (g) |  | |||
| Izinga lokubeka (%) | ||||
| Isisindo esimaphakathi seqanda (g) | ||||
| Isilinganiso sento neqanda | ||||
| Izinga leqanda eliphukile (%) | ||||
| Izinga leqanda elingavamile (%) | ||||
Kuthebula lesi-2, amazinga okubeka awo wonke amaqembu acutshungulwa kusetshenziswa i-diludine ayathuthukiswa ngokusobala, lapho umphumela uma kusetshenzwa ngokusebenzisa i-150mg/kg ulungile (kufika ku-83.36%), futhi u-11.03% (p<0.01) uthuthukisiwe uma uqhathaniswa neqembu lereferensi;ngakho-ke i-diludine inomphumela wokuthuthukisa izinga lokubeka.Uma kubhekwa isisindo esijwayelekile seqanda, isisindo seqanda siyakhula (p>0.05) kanye nokwanda kwe-diludine ekudleni kwansuku zonke.Uma kuqhathaniswa neqembu lereferensi, umehluko phakathi kwazo zonke izingxenye ezicutshunguliwe zamaqembu acutshungulwa ngokusebenzisa i-200mg/kg ye-diludine ayibonakali lapho i-1.79g yokudla okuphakelayo yengezwa ngokwesilinganiso;Nokho, umehluko uba sobala kancane kancane kanye ne-diludine ekhulayo, futhi umehluko wesilinganiso sezinto ezibonakalayo neqanda phakathi kwezingxenye ezicutshungulwayo uyabonakala (p<0.05), futhi umphumela uba muhle uma i-150mg/kg ye-diludine futhi 1.25:1 okwehliswe ngo-10.36% (p<0.01) uma kuqhathaniswa neqembu lesithenjwa.Kubonwa ngesilinganiso seqanda eliphukile lazo zonke izingxenye ezicutshungulwayo, izinga leqanda eliphukile (p<0.05) lingancishiswa lapho i-diludine yengezwe ekudleni kwansuku zonke;futhi iphesenti lamaqanda angajwayelekile liyancipha (p<0.05) kanye nokwanda kwe-diludine.
2.2 Umthelela wekhwalithi yamaqanda
Kubonwa kuThebula lesi-3, inkomba yokuma kweqanda kanye namandla adonsela phansi athile awathinteki (p>0.05) lapho i-diludine yengezwa ekudleni kwansuku zonke, futhi isisindo segobolondo siyanda ngokukhula kwe-diludine yengezwe ekudleni kwansuku zonke, lapho izisindo zamagobolondo zikhushulwa ngo-10.58% kanye no-10.85% (p<0.05) ngokulandelanayo uma kuqhathaniswa namaqembu okubhekisela lapho kufakwa i-150 kanye ne-200mg/kg ye-diludine;ubukhulu begobolondo leqanda buyanda ngokukhula kwe-diludine ekudleni kwansuku zonke, lapho ubukhulu begobolondo leqanda bukhuphuka ngo-13.89% (p<0.05) lapho i-100mg/kg ye-diludine yengezwa uma iqhathaniswa namaqembu okubhekisela, kanye nobukhulu amagobolondo amaqanda akhuphuka ngo-19.44% (p<0.01) kanye no-27.7% (p<0.01) ngokulandelanayo lapho kuhlanganiswa u-150 no-200mg/kg.Iyunithi ye-Haugh (p<0.01) iba ngcono ngokusobala uma i-diludine yengezwa, okubonisa ukuthi i-diludine inomphumela wokugqugquzela ukuhlanganiswa kwe-albumen ewugqinsi yomhlophe kweqanda.I-diludine inomsebenzi wokuthuthukisa inkomba ye-yolk, kodwa umehluko awubonakali ngokusobala (p<0.05).Okuqukethwe kwe-cholesterol yesikhupha seqanda kuwo wonke amaqembu kunomehluko futhi kungancishiswa ngokusobala (p<0.05) ngemva kokwengeza u-150 no-200mg/kg we-diludine.Isisindo esihlobene sesikhupha seqanda sihlukile kwesinye ngenxa yamanani ahlukene e-diludine engeziwe, lapho isisindo esihlobene sesikhupha seqanda sithuthukiswa sibe ngu-18.01% kanye no-14.92% (p<0.05) uma kuqhathaniswa no-150mg/kg no-200mg/kg neqembu lereferensi;ngakho-ke, i-diludine efanelekile inomphumela wokukhuthaza ukuhlanganiswa kwe-yolk yeqanda.
Ithebula 3 Imiphumela ye-diludine kwikhwalithi yamaqanda
| Inani le-diludine elizongezwa (mg/kg) | ||||
| Ikhwalithi yeqanda | 0 | 100 | 150 | 200 |
| Inkomba yokuma kweqanda (%) |  | |||
| Amandla adonsela phansi aqondene neqanda (g/cm3) | ||||
| Isisindo esihlobene segobolondo leqanda (%) | ||||
| Ubukhulu begobolondo leqanda (mm) | ||||
| Iyunithi ye-Haugh (U) | ||||
| Inkomba yesikhupha seqanda (%) | ||||
| I-Cholesterol yeqanda leqanda (%) | ||||
| Isisindo esihlobene sesikhupha seqanda (%) | ||||
2.3 Imiphumela kumaphesenti amafutha esiswini kanye nokuqukethwe kwamafutha esibindi ezinkukhu ezizalelayo
Bheka Umfanekiso 1 kanye noMfanekiso 2 ngemiphumela ye-diludine kumaphesenti wamafutha asesiswini kanye nokuqukethwe kwamafutha esibindi ezinkukhu ezizalelayo.
Umfanekiso 1 Umthelela we-diludine kumaphesenti wamafutha e-bodominal (PAF) ezinkukhu ezizalelayo
| Iphesenti lamafutha e-bodominal | |
| Inani le-diludine elizongezwa |
Umfanekiso 2 Umphumela we-diludine kumafutha esibindi (LF) ezinkukhu ezizalelayo
| Okuqukethwe kwamafutha esibindi | |
| Inani le-diludine elizongezwa |
Kubonwa kuMdwebo 1, amaphesenti wamafutha ezisu zeqembu lokuhlola ancishiselwa ku-8.3% no-12.11% (p<0.05) ngokulandelana lapho i-100 kanye ne-150mg/kg ye-diludine iqhathaniswa neqembu lereferensi, futhi iphesenti lamafutha e-bodominal liyancipha. ngo-33.49% (p<0.01) lapho kufakwa u-200mg/kg we-diludine.Kubonwa kuMdwebo 2, amafutha esibindi (ome ngokuphelele) acutshungulwe ngo-100, 150, 200mg/kg we-diludine ngokulandelana ancipha abe ngu-15.00% (p<0.05), 15.62% (p<0.05) kanye no-27.7% (p< 0.01) ngokulandelanayo uma kuqhathaniswa neqembu eliyisithenjwa;ngakho-ke, i-diludine inomphumela wokunciphisa iphesenti lamafutha e-bodominal kanye nokuqukethwe kwamafutha esibindi kokuqukethwe kokubeka ngokusobala, lapho umphumela ulungile lapho i-200mg / kg ye-diludine yengezwa.
2.4 Umphumela ku-serum biochemical index
Uma kubonwa kuThebula lesi-4, umehluko phakathi kwezingxenye ezicutshungulwe ngesikhathi sokuhlolwa kweSigaba I (30d) sokuhlolwa kwe-SOD awubonakali, futhi izinkomba ze-serum biochemical yawo wonke amaqembu lapho i-diludine yengezwe khona kuSigaba II (80d) sokuhlolwa ziphakeme. kuneqembu lereferensi (p<0.05).I-uric acid (p<0.05) ku-serum ingancishiswa lapho kufakwa u-150mg/kg kanye no-200mg/kg we-diludine;kuyilapho umphumela (p<0.05) utholakala lapho i-100mg/kg ye-diludine yengezwa esigabeni soku-I. I-diludine inganciphisa i-triglyceride ku-serum, lapho umphumela uba ulungile (p<0.01) eqenjini lapho u-150mg/kg I-diludine yengezwe eSigabeni I, futhi isezingeni eliphezulu eqenjini lapho i-200mg/kg ye-diludine yengezwa esiGabeni II.I-cholesterol ephelele ku-serum iyancipha ngokukhula kwe-diludine efakwe ekudleni kwansuku zonke, ikakhulukazi okuqukethwe kwe-cholesterol ephelele ku-serum yehla ngo-36.36% (p<0.01) no-40.74% (p<0.01) ngokulandelanayo lapho i-150mg/kg. kanye no-200mg/kg we-diludine kwengezwe esigabeni soku-I uma kuqhathaniswa neqembu lereferensi, futhi kwehliswa ngo-26.60% (p<0.01), 37.40% (p<0.01) kanye no-46.66% (p<0.01) ngokulandelanayo lapho 100mg/kg, 150mg /kg kanye ne-200mg/kg ye-diludine yengezwe esigabeni sesi-II uma kuqhathaniswa neqembu lereferensi.Ngaphezu kwalokho, i-ALP iyanda ngokukhula kwe-diludine engezwe ekudleni kwansuku zonke, kuyilapho amanani e-ALP eqenjini okufakwa kulo u-150mg/kg no-200mg/kg we-diludine aphakeme kuneqembu lereferensi (p<0.05) ngokusobala.
Ithebula 4 Imiphumela ye-diludine kumapharamitha we-serum
| Inani le-diludine elizongezwa (mg/kg) Esigabeni I (30d) sokuhlolwa | ||||
| Into | 0 | 100 | 150 | 200 |
| I-Superoxide dismutase (mg/mL) |  | |||
| I-Uric acid | ||||
| I-Triglyceride (mmol/L) | ||||
| I-Lipase (U/L) | ||||
| I-Cholesterol (mg/dL) | ||||
| I-Glutamic-pyruvic transaminase (U/L) | ||||
| I-Glutamic-oxalacetic transaminase (U/L) | ||||
| I-alkaline phosphatase (mmol/L) | ||||
| I-calcium ion (mmol/L) | ||||
| I-Inorganic phosphorus (mg/dL) | ||||
| Inani le-diludine elizongezwa (mg/kg) Esigabeni II (80d) sokuhlolwa | ||||
| Into | 0 | 100 | 150 | 200 |
| I-Superoxide dismutase (mg/mL) |  | |||
| I-Uric acid | ||||
| I-Triglyceride (mmol/L) | ||||
| I-Lipase (U/L) | ||||
| I-Cholesterol (mg/dL) | ||||
| I-Glutamic-pyruvic transaminase (U/L) | ||||
| I-Glutamic-oxalacetic transaminase (U/L) | ||||
| I-alkaline phosphatase (mmol/L) | ||||
| I-calcium ion (mmol/L) | ||||
| I-Inorganic phosphorus (mg/dL) | ||||
3 Ukuhlaziya nengxoxo
3.1 I-diludine ekuhlolweni ithuthukise izinga lokuzala, isisindo seqanda, iyunithi ye-Haugh kanye nesisindo esilinganiselwe sesikhupha seqanda, okubonise ukuthi i-diludine ibe nemiphumela yokukhuthaza ukufana kweprotheni kanye nokwenza ngcono inani lokuhlanganiswa kokuqina. i-albhamuen yeqanda elimhlophe kanye namaprotheni e-egg yolk.Ngaphezu kwalokho, okuqukethwe kwe-uric acid ku-serum kwancishiswa ngokusobala;futhi ngokuvamile kwavunywa ukuthi ukuncishiswa kokuqukethwe kwe-nitrogen engeyona amaprotheni ku-serum kwakusho ukuthi isivinini se-catabolism samaprotheni sincishisiwe, futhi isikhathi sokugcinwa kwe-nitrogen sahlehliswa.Lo mphumela unikeze isisekelo sokwandisa ukugcinwa kwamaprotheni, ukukhuthaza ukubeka amaqanda kanye nokwenza ngcono isisindo seqanda lezinkukhu ezizalelayo.Umphumela wokuhlolwa uveze ukuthi umphumela wokubeka ulungile lapho kwengezwa i-150mg/kg ye-diludine, eyayihambisana nomphumela.[6,7]ye-Bao Erqing ne-Qin Shangzhi futhi itholwe ngokungeza i-diludine ngesikhathi sekwephuzile sokubekela izinkukhu.Umphumela wehliswa lapho inani le-diludine lidlula i-150mg/kg, okungenzeka kungenxa yokuthi ukuguqulwa kwamaprotheni.[8]kwathinteka ngenxa yomthamo oweqile kanye nomthwalo oweqile we-metabolism yesitho ku-diludine.
3.2 Ukugxila kwe-Ca2+ku-serum yeqanda lokubekela yancipha, i-P ku-serum yancipha ekuqaleni futhi umsebenzi we-ALP wanda ngokusobala lapho kukhona i-diludine, okubonisa ukuthi i-diludine ithinte i-metabolism ye-Ca no-P ngokusobala.U-Yue Wenbin ubike ukuthi i-diludine ingakhuthaza ukumuncwa[9] izakhi zamaminerali i-Fe ne-Zn;I-ALP yayikhona ikakhulukazi ezicutshini, njengesibindi, ithambo, umgudu wamathumbu, izinso, njll.;I-ALP ku-serum yayisuka esibindini nasethanjeni ikakhulukazi;I-ALP esethanjeni yayikhona ku-osteoblast ikakhulukazi futhi yayikwazi ukuhlanganisa i-phosphate ion ne-Ca2 evela ku-serum ngemva kokuguqulwa ngokukhuthaza ukubola kwe-phosphate nokwandisa ukugcwala kwe-ion phosphate, futhi yafakwa ethanjeni ngendlela ye-hydroxyapatite, njll. . ukuze kuholele ekuncipheni kwe-Ca no-P ku-serum, okuhambisana nokwanda kogqinsi lwegobolondo leqanda kanye nesisindo esilinganiselwe segobolondo leqanda ezinkomba zekhwalithi yeqanda.Ngaphezu kwalokho, izinga leqanda eliphukile kanye nephesenti leqanda elingavamile lancishiswa ngokusobala ngokusebenza kokubeka, okubuye kwachaza leli phuzu.
3.3 Ukufakwa kwamafutha esiswini kanye nokuqukethwe kwamafutha esibindi ezinkukhu ezizalelayo kwancishiswa ngokusobala ngokufaka i-diludine ekudleni, okubonisa ukuthi i-diludine ibe nomthelela wokuvimbela ukuhlanganiswa kwamafutha emzimbeni.Ngaphezu kwalokho, i-diludine ingathuthukisa umsebenzi we-lipase ku-serum ekuqaleni kwesigaba;umsebenzi we-lipase wanda ngokusobala eqenjini lapho kwanezelwa khona i-100mg/kg ye-diludine, futhi okuqukethwe kwe-triglyceride ne-cholesterol ku-serum kwancishiswa (p<0.01), okubonisa ukuthi i-diludine ingakhuthaza ukubola kwe-triglyceride. futhi unciphise ukwakheka kwe-cholesterol.Ukufakwa kwamafutha kungavinjelwa ngenxa yokuthi i-enzyme ye-lipid metabolism esibindini[10,11], kanye nokuncipha kwe-cholesterol esitokisini seqanda nakho kwachaza leli phuzu [13].U-Chen Jufang ubike ukuthi i-diludine inganqanda ukwakheka kwamafutha esilwaneni futhi ithuthukise amaphesenti enyama ezondile yama-broilers nengulube, futhi ibe nomthelela wokwelapha isibindi esinamafutha.Umphumela wokuhlolwa wawucacisa lo mshini wokusebenza, futhi imiphumela yokuhlakaza kanye nokubhekwa kwezinkukhu zokuhlola iphinde yafakazela ukuthi i-diludine inganciphisa izinga lokuvela kwesibindi esinamafutha ezinkukhu ezibekela amaqanda ngokusobala.
3.4 I-GPT ne-GOT yizinkomba ezimbili ezibalulekile ezibonisa ukusebenza kwesibindi nenhliziyo, futhi isibindi nenhliziyo kungase konakaliswe uma imisebenzi yako iphezulu kakhulu.Imisebenzi ye-GPT kanye ne-GOT ku-serum ayizange ishintshwe ngokusobala lapho i-diludine yengezwa ekuhlolweni, okubonisa ukuthi isibindi nenhliziyo akukonakali;ngokuqhubekayo, umphumela wokulinganisa we-SOD ubonise ukuthi umsebenzi we-SOD ku-serum ungathuthukiswa ngokusobala lapho i-diludine isetshenziswa isikhathi esithile.I-SOD ibhekisela ku-scavenger omkhulu we-superoxide free radical emzimbeni;kubaluleke kakhulu ukugcina ubuqotho be-membrane ye-biological, ukuthuthukisa amandla okuzivikela omzimba kanye nokugcina impilo yesilwane lapho okuqukethwe kwe-SOD emzimbeni kwanda.I-Quh Hai, njll. ibike ukuthi i-diludine ingathuthukisa umsebenzi we-6-glucose phosphate dehydrogenase kulwelwesi lwebhayoloji futhi iqinise izicubu [2] zeseli yebhayoloji.U-Sniedze uveze ukuthi i-diludine inqande umsebenzi [4] we-NADPH cytochrome C reductase ngokusobala ngemva kokutadisha ubudlelwano phakathi kwe-diludine kanye ne-enzyme efanelekile ku-NADPH ethize yokudluliswa kwama-electron ku-microsome yesibindi segundane.Ama-odydents aphinde aveza ukuthi i-diludine yayihlobene [4] nesistimu ye-oxidase eyinhlanganisela kanye ne-enzyme ye-microsomal ehlobene ne-NADPH;kanye nendlela yesenzo se-diludine ngemva kokungena esilwaneni ukudlala indima yokumelana ne-oxidation nokuvikela ulwelwesi lwebhayoloji [8] ngokunqamula umsebenzi we-electron yokudlulisa i-enzyme ye-NADPH ye-microsome futhi ivimbele inqubo ye-peroxidation ye-lipid compound.Umphumela wokuhlola wafakazela ukuthi umsebenzi wokuvikela we-diludine kuya kulwelwesi lwebhayoloji usuka ezinguqukweni zomsebenzi we-SOD uye ezinguqukweni zemisebenzi ye-GPT ne-GOT futhi wafakazela imiphumela yocwaningo ye-Sniedze ne-Odydents.
Ireferensi
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2 Qu Hai, Lv Ye, Wang Baosheng, Umphumela we-diludine wengezwe ekudleni kwansuku zonke esilinganisweni sokukhulelwa kanye nekhwalithi yesidoda senyama kanogwaja.J. Chinese Journal of Rabbit Farming1994(6): 6-7
3 Chen Jufang, Yin Yuejin, Liu Wanhan, njll. Ukuhlolwa kokusetshenziswa okunwetshiwe kwe-diludine njengesengezo sokuphakelayoOkuphakelayo Ucwaningo1993 (3): 2-4
4 U-Zheng Xiaozhong, u-Li Kelu, u-Yue Wenbin, njll. Ingxoxo yomphumela wesicelo kanye nendlela yokusebenza ye-diludine njengomgqugquzeli wokukhulisa izinkukhu.Okuphakelayo Ucwaningo1995 (7): 12-13
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Isikhathi sokuthumela: Jun-07-2021