AbstractTijaabada ayaa la sameeyay si loo daraaseeyo saamaynta ay Diludine ku leedahay dhigista waxqabadka iyo tayada beedka ee digaagga iyo habka loo wajaho habka saamaynta iyadoo la go'aaminayo tusmooyinka ukunta iyo cabbirrada serum 1024 digaagga ROM ayaa loo qaybiyay afar kooxood mid kasta oo ka mid ah afar nuqul oo 64 ah. digaag kasta, Kooxaha daawaynta waxay heleen isla cunto Basal ah oo lagu kabay 0, 100, 150, 200 mg/kg diludine siday u kala horreeyaan 80 d.Natiijadu waxay noqotay sidan.Ku darida diludine ee cuntada waxay wanaajisay waxqabadka digaagga, taas oo 150 mg / kg daaweynta ugu fiican;heerka jiifka ayaa kordhay 11.8% (p<0.01), beddelka tirada ukunta waxa hoos u dhacay 10.36% (p<0 01).Miisaanka ukunta ayaa la kordhiyay iyadoo kororka diludine lagu daray.Diludine waxay si weyn hoos ugu dhigtay fiirsashada serum ee uric acid (p<0.01);ku darida diludine waxay si weyn hoos ugu dhigtay serum Ca2+iyo fosfooraska inorganic-ka ah, iyo korodhka dhaqdhaqaaqa alkine phosphatase (ALP) ee serum (p<0.05), sidaas darteed waxay saameyn weyn ku yeelatay yaraynta jabinta ukunta (p <0.05) iyo cillad aan caadi ahayn (p <0.05);diludine waxay si weyn u kordhisay dhererka albumenka.Qiimaha haugh (p <0.01), dhumucda qolofta iyo miisaanka qolofka (p< 0.05), 150 iyo 200mg/kg diludine waxay sidoo kale hoos u dhigtay wadarta kolestaroolka ukunta ukunta (p<0.05), laakiin korodhay miisaanka jaallada beedka (p <0.05).Intaa waxaa dheer, diludine waxay kor u qaadi kartaa dhaqdhaqaaqa lipase (p <0.01), waxayna hoos u dhigtaa maadada triglyceride (TG3) (p<0.01) iyo kolestaroolka (CHL) (p<0 01) ee serum, waxay hoos u dhigtay boqolkiiba baruurta caloosha. (p<0.01) iyo dufanka beerka ku jira (p<0.01), waxay awood u lahaayeen inay ka hortagaan digaagga beerka dufanka leh.Diludine waxay si weyn u kordhisay dhaqdhaqaaqa SOD ee serum (p<001) markii lagu daray cuntada in ka badan 30d.Si kastaba ha ahaatee, wax farqi weyn ah lagama helin dhaqdhaqaaqyada GPT iyo GOT ee serum ee u dhexeeya kooxda la daweeyay.Waxaa la qiimeeyay in diludine ay ka hortagi karto xuubka unugyada oksida
Erayada muhiimka ahDiludine;digaag;SOD;kolestaroolka;triglyceride, lipase
Diludine-ku waa ka-hortagga-oxidation-ka cusub ee fitamiin-ka-hortagga ah ee aan nafaqo lahayn wuxuuna leeyahay saameyntiisa[1-3]1970-meeyadii, khabiirkii beeraha ee Latvia ee ka tirsanaan jiray Midowga Soofiyeeti wuxuu ogaaday in diludine ay leedahay saameynteeda.[4]kor u qaadida koritaanka digaaga iyo iska caabinta barafowga iyo gabowga dhirta qaarkood.Waxa la sheegay in diludine-ku aanu kaliya kor u qaadin korriinka xoolaha, balse uu si cad u wanaajin karo waxqabadka taranka xoolaha, isla markaana uu wanaajiyo heerka uurka, soosaarka caanaha, soosaarka ukunta iyo heerka dillaaca xoolaha dheddigga.[1, 2, 5-7].Daraasada diludine ee Shiinaha ayaa la bilaabay laga bilaabo 1980-meeyadii, inta badan daraasadaha ku saabsan diludine ee Shiinaha waxay ku kooban yihiin isticmaalka ilaa hadda, tijaabooyinka yar ee shimbiraha ayaa la soo sheegay.Chen Jufang (1993) ayaa sheegay in diludine ay hagaajin karto wax soo saarka ukunta iyo miisaanka ukunta digaaga, laakiin ma qotomin.[5]daraasadda habka hawl-qabadkeeda.Sidaa darteed, waxaan hirgelinay daraasad nidaamsan oo ku saabsan saameynta iyo habka ay ku jirto iyadoo la quudinayo digaagga jiifa cuntada lagu daro diludine-ka, qayb ka mid ah natiijada hadda ayaa lagu soo waramayaa sida soo socota:
Shaxda 1 Halabuurka iyo qaybaha nafaqada ee cuntada tijaabada ah
%
----- -----
Halabuurka cuntada qaybaha nafaqada
----- -----
Galleyda 62 ME③ 11.97
Saxarka bean 20 CP 17.8
Cuntada kalluunka 3 Ca 3.42
Cuntada la kufsaday 5 P 0.75
Cunto lafaha 2 M et 0.43
Cunto dhagax ah 7.5 M iyo Cys 0.75
Methionine 0.1
Cusbada 0.3
Multivitamin ① 10
Walxaha raadraaca ② 0.1
----- ----------------------------------
① Multivitamin: 11mg of riboflavin, 26mg of folic acid, 44mg of oryzanin, 66mg of niacin, 0.22mg of biotin, 66mg of B6, 17.6ug of B12, 880mg of choline, 30mg of VK, 66IUE, 6600ICU ee VDiyo 20000 ICU ee VA, ayaa lagu daraa kiilo kasta oo cuntada ah;iyo 10g multivitamin ayaa lagu daraa 50kg kasta oo cunto ah.
② walxaha raadraaca (mg/kg): 60 mg ee Mn, 60mg of Zn, 80mg of Fe, 10mg of Cu, 0.35mg of I iyo 0.3mg of Se waxaa lagu daraa kiilo kasta oo cuntada ah.
③ Unugga tamarta dheef-shiid kiimikaadka waxaa loola jeedaa MJ/kg.
1. Qalabka iyo habka
1.1 Qalabka tijaabada
Beijing Sunpu Biochem.& FarsamadaCo., Ltd. waa inay bixisaa diludine;iyo xayawaanka tijaabada ah waa in ay tixraacaan digaagga ganacsiga ee Roomaanka kuwaas oo jira 300 maalmood.
Cunto tijaabo ah: cuntada tijaabada tijaabada ah waa in loo diyaariyaa iyadoo loo eegayo xaalada dhabta ah inta lagu jiro wax soo saarka iyadoo lagu salaynayo heerka NRC, sida ku cad shaxda 1.
1.2 Habka tijaabada
1.2.1 Tijaabada quudinta: tijaabada quudinta waa in laga hirgeliyaa beerta Shirkadda Hongji ee Magaalada Jiande;1024 Digaagga jiifka Roomaanka waa in la doortaa oo loo qaybiyaa afar kooxood si aan kala sooc lahayn mid walbana waa 256 xabbo (koox kasta waa in lagu celiyaa afar jeer, digaag kastana waa in lagu celiyaa 64 jeer);digaagga waa in lagu quudiyaa afarta cunto ee kala duwan ee diludine, iyo 0, 100, 150, 200mg/kg ee quudinta waa in lagu daraa koox kasta.Imtixaanku wuxuu bilaabmay Abriil 10, 1997;Digaaggana waxay heli kareen cunto oo si xor ah ayay biyaha u qaadan karaan.Raashinka ay qaataan koox kasta, heerka dhalidda, wax-soo-saarka ukunta, ukunta jabtay iyo tirada beedka aan caadiga ahayn waa in la diwaangeliyaa.Waxaa intaa dheer, imtixaanku wuxuu dhammaaday Juun 30, 1997.
1.2.2 Cabbirka tayada ukunta: 20 ukun waa in si aan kala sooc lahayn loo qaato marka tijaabada la hirgaliyo afar 40 maalmood si loo cabbiro tilmaamayaasha la xidhiidha tayada ukunta, sida tusmada qaabka ukunta, unugga haugh, miisaanka qolofka, dhumucdiisuna waxay tahay qolof, index-ka jaallo ah, qaraabo miisaanka jaallo ah, iwm. Intaa waxaa dheer, content ee kolestaroolka ee jaallo ah waa in lagu cabbiraa iyadoo la isticmaalayo habka COD-PAP joogitaanka reagent Cicheng soo saaray by Ningbo Cixi Biochemical Plant.
1.2.3 Cabbirka serum biochemical index: 16 digaag oo tijaabo ah waa in laga soo qaadaa koox kasta mar kasta oo baaritaanka la sameeyay 30 maalmood iyo marka baaritaanka la dhammeeyo si loo diyaariyo serum ka dib marka laga soo qaado dhiigga xididdada garabka.Serumka waa in lagu kaydiyaa heerkul hooseeya (-20℃) si loo cabbiro tusmooyinka biochemical ee khuseeya.Boqolkiiba baruurta caloosha iyo waxa ku jira dufanka beerka waa in la cabbiraa ka dib marka la gowraco oo la soo saaro baruurta caloosha iyo beerka marka la dhammeeyo tijaabada dhiigga.
Dismutase-ka superoxide (SOD) waa in lagu cabbiraa iyadoo la adeegsanayo habka korantada iyadoo ay jirto xirmada reagent ee ay soo saartay Beijing Huaqing Biochem.& FarsamadaMachadka Cilmi-baarista.Uric acid (UN) ee ku jira serumka waa in lagu cabbiraa iyadoo la isticmaalayo habka U ricase-PAP iyadoo ay jirto xirmada reagent ee Cicheng;triglyceride (TG3) waa in lagu cabbiraa iyadoo la isticmaalayo GPO-PAP hal-tallaabo oo ah xirmada reagent ee Cicheng;lipase waa in lagu cabbiraa iyadoo la isticmaalayo nephelometry iyadoo ay jirto xirmada reagent ee Cicheng;wadarta guud ee kolestaroolka (CHL) waa in lagu cabbiraa iyadoo la isticmaalayo habka COD-PAP iyadoo ay jirto xirmada reagent ee Cicheng;transaminase glutamic-pyruvic (GPT) waa in lagu cabbiraa iyadoo la isticmaalayo midabka midabka iyadoo ay jirto xirmada reagent ee Cicheng;glutamic-oxalacetic transaminase (GOT) waa in lagu cabbiraa iyadoo la isticmaalayo midabka midabka iyadoo ay jirto xirmada reagent ee Cicheng;phosphatase alkaline (ALP) waa in lagu cabbiraa iyadoo la isticmaalayo habka heerka joogitaanka xirmada reagent Cicheng;ion calcium (Ca2+) ee serum waa in lagu cabbiraa iyadoo la isticmaalayo habka isku dhafan ee methylthymol buluug iyadoo ay jirto xirmada reagent ee Cicheng;Fosfooraska inorganic (P) waa in lagu cabbiraa iyadoo la isticmaalayo habka buluuga ah ee molybdate iyadoo ay jirto xirmada reagent ee Cicheng.
2 Natiijada tijaabada
2.1 Saamaynta shaqo dhigista
Bandhigyada bandhigyada kooxaha kala duwan ee lagu farsameeyay isticmaalka diludine ayaa lagu muujiyay shaxda 2.
Shaxda 2aad Waxqabadka digaagga lagu quudiyo cunto asal ah oo lagu kabo afar heer oo diludine ah
| Qadarka diludine ee lagu kordhinayo (mg/kg) | ||||
| 0 | 100 | 150 | 200 | |
| Qaadashada quudinta (g) |  | |||
| Heerka dhigista (%) | ||||
| Celceliska miisaanka ukunta (g) | ||||
| Saamiga walxaha ilaa ukunta | ||||
| Heerka ukunta jaban (%) | ||||
| Heerka ukunta aan caadiga ahayn (%) | ||||
Laga soo bilaabo Shaxda 2, heerarka dhigista dhammaan kooxaha lagu farsameeyay isticmaalka diludine si cad ayaa loo wanaajiyey, taas oo saameynta marka la isticmaalo 150mg / kg ay tahay mid aad u fiican (ilaa 83.36%), iyo 11.03% (p <0.01) waa la hagaajiyay marka la barbardhigo. oo leh kooxda tixraaca;Sidaa darteed diludine-ku wuxuu leeyahay saamaynta hagaajinta heerka jiifka.Marka la eego celceliska miisaanka ukunta, miisaanka ukunku wuu kordhayaa (p>0.05) oo ay weheliso kordhinta diludine ee cuntada maalinlaha ah.Marka la barbardhigo kooxda tixraaca, farqiga u dhexeeya dhammaan qaybaha la warshadeeyay ee kooxaha lagu farsameeyay iyadoo la adeegsanayo 200mg / kg ee diludine maaha mid cad marka 1.79g ee qaadashada quudinta lagu daro celcelis ahaan;si kastaba ha ahaatee, farqiga ayaa si tartiib tartiib ah u muuqda iyada oo ay weheliso diludine sii kordhaya, iyo farqiga u dhexeeya saamiga walxaha iyo ukunta ee qaybaha la warshadeeyay waa mid cad (p <0.05), saameyntuna waa mid aad u fiican marka 150mg / kg ee diludine iyo waa 1.25:1 kaas oo hoos loo dhigay 10.36% (p<0.01) marka la barbar dhigo kooxda tixraaca.Marka la eego heerka ukunta jabtay ee dhammaan qaybaha la warshadeeyay, heerka jabka ukunta (p<0.05) waa la dhimi karaa marka diludine lagu daro cuntada maalinlaha ah;iyo boqolkiiba ukunta aan caadiga ahayn waa la dhimay (p<0.05) iyadoo la kordhinayo diludine.
2.2 Saamaynta tayada ukunta
Marka la eego shaxda 3, index qaabka ukunta iyo cuf-jiidka gaarka ah ee ukunta ma saameynayaan (p>0.05) marka diludine lagu daro cuntada maalinlaha ah, iyo miisaanka qolofka ayaa kordhay iyada oo la kordhinayo diludine lagu daro cuntada maalinlaha ah. halkaas oo miisaanka qolofka lagu kordhiyo 10.58% iyo 10.85% (p<0.05) siday u kala horreeyaan marka la barbardhigo kooxaha tixraaca marka 150 iyo 200mg / kg ee diludine lagu daro;Dhumucda qolofta ukunta ayaa la kordhiyaa iyada oo ay weheliso kordhinta diludine ee cuntada maalinlaha ah, taas oo dhumucda qolofta ukunta ay korodhay 13.89% (p<0.05) marka 100mg / kg ee diludine lagu daro marka la barbardhigo kooxaha tixraaca, iyo dhumucda qolofka ukunta waxa la kordhiyey 19.44% (p<0.01) iyo 27.7% (p<0.01) siday u kala horreeyaan marka 150 iyo 200mg/kg lagu daro.Unugga Haugh (p <0.01) ayaa si cad loo hagaajiyaa marka diludine lagu daro, taas oo muujinaysa in diludine ay leedahay saamaynta kor u qaadida synthesize albumen qaro weyn oo ukun cad.Diludine-ku wuxuu leeyahay shaqada hagaajinta index of yolk, laakiin farqiga u maaha mid cad (p <0.05).Waxyaabaha ku jira kolestaroolka ukunta ukunta ee kooxaha oo dhan way kala duwan yihiin waxaana si cad loo dhimi karaa (p<0.05) ka dib marka lagu daro 150 iyo 200mg / kg ee diludine.Miisaanka qaraabada ah ee ukunta ukunta way ka duwan yihiin midba midka kale sababtoo ah xaddi kala duwan oo diludine ah oo lagu daray, taas oo miisaanka qaraabada ee ukunta ukunta la hagaajiyay 18.01% iyo 14.92% (p <0.05) marka 150mg/kg iyo 200mg/kg la barbardhigo. oo leh kooxda tixraaca;Sidaa darteed, diludine ku habboon ayaa saameyn ku leh kor u qaadida isku-dhafka ukunta ukunta.
Shaxda 3 Saamaynta diludine ee tayada ukunta
| Qadarka diludine ee lagu kordhinayo (mg/kg) | ||||
| Tayada ukunta | 0 | 100 | 150 | 200 |
| Tusmada qaabka ukunta (%) |  | |||
| Cuf-jiid gaar ah Ukun (g/cm3) | ||||
| Miisaanka qolofta ukunta (%) | ||||
| Dhumucda qolofta ukunta (mm) | ||||
| Unugga Haugh (U) | ||||
| Tusmada ukunta ukunta (%) | ||||
| Kolestaroolka ukunta ukunta (%) | ||||
| Miisaanka qaraabada ah ee ukunta ukunta (%) | ||||
2.3 Saamaynta boqolkiiba baruurta caloosha iyo waxa ku jira baruurta beerka ee digaagga jiifa
Fiiri sawirka 1 iyo sawirka 2 saamaynta diludine ilaa boqolkiiba baruurta caloosha iyo waxa ku jira baruurta beerka ee digaagga jiifa
Jaantuska 1 Saamaynta diludine ee boqolkiiba dufanka abodominal (PAF) ee digaagga jiifa
| Boqolkiiba dufanka abodominalka | |
| Qadarka diludine ee lagu daro |
Jaantuska 2 Saamaynta diludine ee dufanka beerka (LF) ee digaagga jiifa
| Dufanka beerka ku jira | |
| Qadarka diludine ee lagu daro |
Marka la eego Jaantuska 1, boqolleyda dufanka abodominal ee kooxda tijaabada ayaa la dhimay 8.3% iyo 12.11% (p<0.05) siday u kala horreeyaan marka 100 iyo 150mg / kg diludine la barbar dhigo kooxda tixraaca, iyo boqolkiiba baruurta abodominal waa la dhimay. 33.49% (p <0.01) marka 200mg / kg ee diludine lagu daro.Marka laga eego jaantuska 2, waxa ku jira dufanka beerka (gabi ahaanba engegan) oo lagu farsameeyay 100, 150, 200mg/kg ee diludine siday u kala horreeyaan ayaa loo dhimay 15.00% (p <0.05), 15.62% (p <0.05) iyo 27.7% (p<0.05) 0.01) siday u kala horreeyaan marka la barbar dhigo kooxda tixraaca;Sidaa darteed, diludine-ku waxay leedahay saamaynta hoos u dhigista boqolkiiba dufanka abodominal iyo dufanka beerka ee ku jira waxa ku jira si cad, taas oo saameyntu ay tahay mid aad u fiican marka 200mg / kg ee diludine lagu daro.
2.4 Saamaynta serum biochemical index
Marka laga eego shaxda 4, farqiga u dhexeeya qaybaha la warshadeeyay inta lagu gudajiray Wajiga I (30d) ee baaritaanka SOD ma cadda, iyo tusmooyinka biochemical ee serum ee dhammaan kooxaha ay diludine-ka lagu daro Wajiga II (80d) ee tijaabada ayaa ka sarreeya. marka loo eego kooxda tixraaca (p<0.05).Uric acid (p <0.05) ee serum waa la dhimi karaa marka 150mg / kg iyo 200mg / kg ee diludine lagu daro;halka saamaynta (p <0.05) la heli karo marka 100mg / kg diludine lagu daro Wajiga I. Diludine waxay yareyn kartaa triglyceride ee serum, taas oo saameyntu ay tahay mid aad u fiican (p <0.01) kooxda marka 150mg / kg ee diludine waxaa lagu daraa Wajiga I, waxayna ugu fiican tahay kooxda marka 200mg/kg oo diludine lagu daro Wajiga II.Wadarta kolestaroolka ee serum waa la dhimay iyadoo la kordhinayo diludine lagu daro cuntada maalinlaha ah, gaar ahaan waxa ku jira wadarta kolestaroolka ee serum waxaa la dhimay 36.36% (p <0.01) iyo 40.74% (p <0.01) siday u kala horreeyaan marka 150mg/kg iyo 200mg / kg diludine ayaa lagu daraa Wajiga I marka la barbardhigo kooxda tixraaca, waxaana la dhimay 26.60% (p <0.01), 37.40% (p <0.01) iyo 46.66% (p <0.01) siday u kala horreeyaan marka 100mg/kg, 150mg /kg iyo 200mg/kg oo diludine ah ayaa lagu daraa Wajiga II marka la barbar dhigo kooxda tixraaca.Waxaa intaa dheer, ALP waxaa la kordhiyaa iyada oo la kordhinayo diludine lagu daro cuntada maalinlaha ah, halka qiyamka ALP ee kooxda oo 150mg / kg iyo 200mg / kg diludine lagu daray ay ka sarreeyaan kooxda tixraaca (p <0.05) cad.
Shaxda 4 Saamaynta diludine ee xuduudaha serum
| Qadarka diludine ee lagu daro (mg/kg) ee Wajiga I (30d) ee tijaabada | ||||
| Shayga | 0 | 100 | 150 | 200 |
| Superoxide dismutase (mg/mL) |  | |||
| Uric acid | ||||
| Triglyceride (mmol/L) | ||||
| Lipase (U/L) | ||||
| Kolestaroolka (mg/dL) | ||||
| Glutamic-pyruvic transaminase (U/L) | ||||
| Glutamic-oxalacetic transaminase (U/L) | ||||
| Fosfatase alkaline (mmol/L) | ||||
| Ion kalsiyum (mmol/L) | ||||
| Fosfooraska aan organic ahayn (mg/dL) | ||||
| Qadarka diludine ee lagu daro (mg/kg) ee Wajiga II (80d) ee tijaabada | ||||
| Shayga | 0 | 100 | 150 | 200 |
| Superoxide dismutase (mg/mL) |  | |||
| Uric acid | ||||
| Triglyceride (mmol/L) | ||||
| Lipase (U/L) | ||||
| Kolestaroolka (mg/dL) | ||||
| Glutamic-pyruvic transaminase (U/L) | ||||
| Glutamic-oxalacetic transaminase (U/L) | ||||
| Fosfatase alkaline (mmol/L) | ||||
| Ion kalsiyum (mmol/L) | ||||
| Fosfooraska aan organic ahayn (mg/dL) | ||||
3 Falanqaynta iyo falanqaynta
3.1 Diludine-ka ku jira tijaabada ayaa wanaajisay heerka jiifka, miisaanka ukunta, unugga Haugh iyo miisaanka qaraabada ee jaallada, taas oo muujisay in diludine ay leedahay saamaynta kor u qaadida milmaynta borotiinka iyo hagaajinta qadarka isku dhafka dhumucda albumen oo ah ukun cad iyo borotiin ah ukun jaallo ah.Dheeraad ah, waxa ku jira aashitada uric ee serum si cad ayaa loo dhimay;waxaana guud ahaan la isla qiray in hoos u dhigista maadada nitrogen ee aan borotiinka ahayn ee serum ay ka dhigan tahay in xawaaraha catabolism ee borotiinka la dhimay, iyo wakhtiga haynta nitrogen dib loo dhigay.Natiijadu waxay siisay aasaaska korodhka sii-haysashada borotiinka, kor u qaadista ukunta iyo hagaajinta miisaanka ukunta digaagga.Natiijadii imtixaanku waxay tilmaantay in saamaynta jiifku ay tahay mid aad u wanaagsan marka 150mg/kg ee diludine lagu daro, taas oo asal ahaan la socota natiijada.[6,7]Bao Erqing iyo Qin Shangzhi oo lagu helay iyadoo lagu daray diludine xilliga dambe ee digaagga.Saamaynta ayaa la dhimay marka qadarka diludine uu ka sarreeyo 150mg / kg, taas oo noqon karta sababtoo ah isbeddelka borotiinka[8]waxaa saameeyay qiyaasta xad dhaafka ah iyo culeyska xad-dhaafka ah ee dheef-shiid kiimikaad ee xubinta ilaa diludine-ka.
3.2 Xoog saarista Ca2+Serum ee ugxanta ayaa la dhimay, P ee serum ayaa la yareeyay bilawgii iyo waxqabadka ALP ayaa si cad u kordhay joogitaanka diludine, taas oo muujisay in diludine ay saamaysay dheef-shiid kiimikaadka Ca iyo P si cad.Yue Wenbin ayaa sheegay in diludine ay kor u qaadi karto nuugista[9] xubno ka mid ah macdanta Fe iyo Zn;ALP waxay inta badan ka jirtay unugyo, sida beerka, lafta, mareenka mindhicirka, kelyaha, iwm.;ALP ee serumku waxa uu ka yimi beerka iyo lafaha inta badan;ALP ee lafta waxay ku jirtay osteoblasst inta badan waxayna isku dari kartaa fosfate ion iyo Ca2 ka serum ka dib isbeddelka iyada oo kor u qaadeysa kala-goynta fosfatka iyo kordhinta xoojinta fosfooraska ion, waxaana lagu dhejiyay lafta qaab hydroxyapatite, iwm. .Waxaa intaa dheer, heerka ukunta jabay iyo boqolkiiba ukunta aan caadiga ahayn ayaa si cad loo dhimay marka la eego waxqabadka dhigista, kaas oo sidoo kale sharaxay qodobkan.
3.3 Dufanka caloosha iyo dufanka beerka ee digaagga jiifa ayaa si cad loo dhimay iyadoo lagu daray diludine cuntada, taas oo muujisay in diludine ay saameyn ku leedahay xakamaynta isku-dhafka dufanka ee jirka.Dheeraad ah, diludine waxay hagaajin kartaa dhaqdhaqaaqa lipase ee serum marxaladda hore;Dhaqdhaqaaqa lipase ayaa si cad u kordhay kooxda 100mg / kg ee diludine lagu daray, iyo waxa ku jira triglyceride iyo kolestaroolka serum waa la dhimay (p<0.01), taas oo muujisay in diludine ay kor u qaadi karto kala-goynta triglyceride iyo in la xakameeyo isku dhafka kolestaroolka.Dhigista baruurta waa la xakameyn karaa sababtoo ah enzyme-ka dheef-shiid kiimikaad ee beerka[10,11], iyo hoos u dhigista kolestaroolka ee ukunta ukunta ayaa sidoo kale sharaxay qodobkan [13].Chen Jufang ayaa sheegay in diludine ay xakamayso samaynta dufanka xayawaanka iyo hagaajinta boqolkiiba hilibka baruurta ee broilers iyo doofaarka, oo ay leedahay saamaynta daaweynta beerka dufanka leh.Natiijadii imtixaanku waxay caddeeyeen habkan ficilka ah, natiijooyinka kala-soocidda iyo fiirsashada ee digaagga tijaabada waxay sidoo kale caddeeyeen in diludine ay yarayn karto heerka dhacdooyinka beerka dufanka leh ee digaagga jiifa si cad.
3.4 GPT iyo GOT waa laba tilmaame oo muhiim ah oo ka tarjumaya shaqada beerka iyo wadnaha, beerka iyo wadnahana waxaa laga yaabaa inay dhaawacmaan haddii hawlahoodu aad u sarreeyaan.Dhaqdhaqaaqyada GPT iyo GOT ee serum si cad looma beddelin marka diludine lagu daro baaritaanka, taas oo muujisay in beerka iyo wadnaha aysan waxyeello u geysan;Intaa waxaa dheer, natiijada cabbirka ee SOD waxay muujisay in dhaqdhaqaaqa SOD ee serum uu si cad u wanaajin karo marka diludine loo isticmaalo wakhti go'an.SOD waxa ay tilmaamaysaa qashin-qubka wayn ee xagjirka xorta ah ee superoxide ee jidhka;Waxay muhiim u tahay ilaalinta daacadnimada xuubka noolaha, hagaajinta awoodda difaaca jirka iyo ilaalinta caafimaadka xayawaanka marka waxa ku jira SOD ee jirka la kordhiyo.Quh Hai, iwm. ayaa sheegay in diludine ay hagaajin karto waxqabadka 6-glucose phosphate dehydrogenase ee xuubka noolaha iyo xasilinta unugyada [2] unugga noolaha.Sniedze wuxuu tilmaamay in diludine ay xakamaysay dhaqdhaqaaqa [4] ee NADPH cytochrome C reductase si cad ka dib markii ay daraasad ku samaysay xidhiidhka ka dhexeeya diludine iyo ensaymka khuseeya ee NADPH silsiladda wareejinta elektarooniga ah ee microsome beerka jiirka.Odydents ayaa sidoo kale tilmaamay in diludine ay la xiriirto [4] nidaamka oxidase ee isku dhafan iyo enzyme-ka microsomal ee la xidhiidha NADPH;iyo habka ficilka diludine ka dib marka la galo xayawaanka waa in la ciyaaro doorka ka hortagga oksaydhka iyo ilaalinta xuubka noolaha [8] iyada oo la xakameynayo dhaqdhaqaaqa wareejinta elektaroonigga ah ee NADPH enzyme ee microsome iyo xakamaynta habka peroxidation ee xarunta dufanka.Natiijadu waxay caddeeyeen in shaqada ilaalinta diludine ilaa xuubka noolaha laga bilaabo isbeddelada dhaqdhaqaaqa SOD ee isbeddelka hawlaha GPT iyo GOT oo caddeeyey natiijooyinka daraasadda Sniedze iyo Odydents.
Tixraac
1 Zhou Kai, Zhou Mingjie, Qin Zhongzhi, iwm. Barashada diludine ee hagaajinta waxqabadka taranka idahaJ. Caws iyoLivestock 1994 (2): 16-17
2 Qu Hai, Lv Ye, Wang Baosheng, Saamaynta diludine ee lagu daray cunto maalmeedka heerka uurka iyo tayada shahwada ee bakaylaha hilibka.J. Jariidada Shiinaha ee Beeraha Bakaylaha1994 (6): 6-7
3 Chen Jufang, Yin Yuejin, Liu Wanhan, iwmCilmi-baarista Quudinta1993 (3): 2-4
4 Zheng Xiaozhong, Li Kelu, Yue Wenbin, iwm.Cilmi-baarista Quudinta1995 (7): 12-13
5 Chen Jufang, Yin Yuejin, Liu Wanhan, iwmCilmi-baarista Quudinta1993 (3): 2-5
6 Bao Erqing, Gao Baohua, Tijaabada diludine ee quudinta shimbiraha PekingCilmi-baarista Quudinta1992 (7): 7-8
7 Qin Shangzhi Tijaabada hagaajinta wax soo saarka digaaga hilibka doofaarka xilliga dambe ee jiifinta iyadoo la isticmaalayo diludineWargeyska Guangxi ee Xannaanada Xoolaha & Daawada Xoolaha1993.9 (2): 26-27
8 Dibner J Jl Lvey FJ borotiinka cagaarshowga iyo dheef-shiid kiimikaadka amino acid ee digaaga Cilmiga digaaga1990.69(7): 1188-1194
9 Yue Wenbin, Zhang Jianhong, Zhao Peie, iwm. Barashada ku darida diludine iyo Fe-Zn ee cuntada maalinlaha ah ee digaagga.Quudinta & Xoolaha1997, 18 (7): 29-30
Mildner Ana M
11 W alzon RL Smon C, M orishita T, et aCilmiga digaaga,1993 72 (8): 1479- 1491
12 Donaldson WE Dheef-shiid kiimikaad ee beerka chicks jawaabta quudintaCilmiga digaaga.1990, 69 (7): 1183- 1187
13 Ksiazk ieu icz J.K ontecka H, H ogcw sk i L Qoraal ku saabsan kolestaroolka dhiigga sida tilmaame dufanka jidhka ee shinbiraha.Joornaalka Sayniska Xoolaha iyo Quudinta,1992, 1 (3/4): 289- 294
Waqtiga boostada: Juun-07-2021