AbstractUvavanyo lwenziwa ukufunda iziphumo ze-diludine ekubekeni ukusebenza kunye nomgangatho weqanda kwizikhukukazi kunye nokusondela kwindlela yeziphumo ngokumisela izalathi zeqanda kunye ne-serum parameters I-1024 ROM izikhukhukazi zahlulwe zangamaqela amane ngalinye libandakanya ii-replicates ezine ze-64 izikhukhukazi nganye, Amaqela onyango afumene ukutya okusisiseko okufanayo kongezwa nge-0, 100, 150, 200 mg/kg diludine ngokulandelelanayo kwi-80 d.Iziphumo zaba ngolu hlobo lulandelayo.Ukongezwa kwe-diludine ekutyeni kuphuculwe ukusebenza kweenkukhu, apho unyango lwe-150 mg / kg lwalungcono;izinga layo lokulala liye landa nge-11.8% (p<0.01), ukuguqulwa kweqanda leqanda liye lancipha ngo-10.36% (p<0 01).Ubunzima beqanda bonyuswa ngokunyuka kwe-diludine yongezwa.I-Diludine iyancipha kakhulu i-serum concentration ye-uric acid (p <0.01);ukongeza i-diludine yehla kakhulu i-serum Ca2+kunye nomxholo we-phosphate we-inorganic, kunye nomsebenzi owandisiweyo we-alkine phosphatase (ALP) ye-serum (p<0.05), ngoko ibe nemiphumo ebalulekileyo ekunciphiseni ukuphuka kweqanda (p <0.05) kunye nokungaqhelekanga (p <0.05);i-diludine inyuse kakhulu ukuphakama kwe-albumen.Ixabiso le-Haugh (p <0.01), ubukhulu beqokobhe kunye nobunzima beqokobhe (p<0.05), i-150 kunye ne-200mg/kg i-diludine nayo iyancipha i-choleasterol epheleleyo kwi-yolk yeqanda (p<0 05), kodwa ukunyuka kwe-egg yolk ubunzima (p <0.05).Ukongezelela, i-diludine inokuphucula umsebenzi we-lipase (p <0.01), kwaye iyancipha umxholo we-triglyceride (TG3) (p <0.01) kunye ne-cholesterol (CHL) (p <0 01) kwi-serum, yanciphisa ipesenti yamafutha esiswini. (p<0.01) kunye nomxholo we-fat fat (p<0.01), wayenamandla okuthintela iinkukhu kwisibindi esinamafutha.I-Diludine yandisa kakhulu umsebenzi we-SOD kwi-serum (p<0 01) xa yongezwa ekudleni okungaphezulu kwe-30d.Nangona kunjalo, akukho mahluko ubalulekileyo ofunyenwe kwimisebenzi ye-GPT kunye ne-GOT ye-serum phakathi kweqela lolawulo kunye nelonyango.Kwaxelwa ukuba i-diludine inokuthintela inwebu yeeseli kwi-oxidation
Amagama aphambiliiDiludine;inkukhu;I-SOD;cholesterol;triglyceride, lipase
I-diludine yinoveli ye-non-nutritive anti-oxidation ye-vitamin additive kwaye ineziphumo.[1-3]Ukuthintela i-oxidation ye-membrane yebhayoloji kunye nokuzinzisa izicubu zeeseli zebhayoloji, njl.njl. Ngeminyaka yee-1970, ingcali yezolimo yaseLatvia kwindawo eyayisakuba yiSoviet Union yafumanisa ukuba idiludine ineziphumo zayo.[4]yokukhuthaza ukukhula kweenkukhu kunye nokumelana neqhwa kunye nokuguga kwezinye izityalo.Kwaxelwa ukuba i-diludine ayinakukhuthaza ukukhula kwesilwanyana kuphela, kodwa iphucula ukusebenza kwezilwanyana ngokucacileyo kwaye iphucule izinga lokukhulelwa, imveliso yobisi, imveliso yeqanda kunye nesantya sokuqanduselwa kwesilwanyana sasetyhini.[1, 2, 5-7].Uphononongo lwe-diludine e-china lwaqalwa ukusuka koo-1980, kwaye uninzi lwezifundo malunga ne-diludine e-China luvalelwe ukusebenzisa isiphumo ukuza kuthi ga ngoku, kwaye izilingo ezimbalwa zokubeka intaka zaxelwa.U-Chen Jufang (1993) ubike ukuba i-diludine inokuphucula imveliso yeqanda kunye nobunzima beqanda lenkukhu, kodwa ayizange ibe nzulu.[5]uphononongo lwendlela yokusebenza kwayo.Ke ngoko, siphumeze uphononongo olucwangcisiweyo lwesiphumo kunye nendlela yokondla iinkukhu ezizalelayo ngokutya okufakwe i-diludine, kwaye enye inxalenye yesiphumo ngoku ichazwe ngolu hlobo lulandelayo:
Itheyibhile yoku-1 Ukuqulunqwa kunye namalungu esondlo sovavanyo lokutya
%
------------------------------------------------- -------------------------------------------
Ukuqulunqwa kokutya Amacandelo ezondlo
------------------------------------------------- -------------------------------------------
Umbona 62 ME③ 11.97
I-pulp yebhontshisi 20 CP 17.8
Ukutya kweentlanzi 3 Ca 3.42
Isidlo sokudlwengulwa 5 P 0.75
Isidlo samathambo 2 M et 0,43
Ukutya kwamatye 7.5 M et Cys 0,75
I-Methionine 0.1
Ityuwa 0.3
I-Multivitamin① 10
Landela amanqaku② 0.1
------------------------------------------------- ----------------------------------------
① Multivitamin: 11mg ye riboflavin, 26mg ye folic acid, 44mg ye oryzanin, 66mg niacin, 0.22mg ye biotin, 66mg ye B6, 17.6ug ye B12, 880mg ye choline, 30mg ye VK, 66IUE, 6600ICU yeVDkunye ne-20000ICU ye-VA, zongezwa kwikhilogram nganye yokutya;kunye ne-10g ye-multivitamin yongezwa kwi-50kg nganye yokutya.
② Ukulandelela izinto (mg / kg): 60 mg ye-Mn, 60mg ye-Zn, 80mg ye-Fe, 10mg ye-Cu, i-0.35mg ye-I kunye ne-0.3mg ye-Se yongezwa kwikhilogram nganye yokutya.
③ Iyunithi yamandla e-metabolizable ibhekisa kwi-MJ/kg.
1. Izinto eziphathekayo kunye nendlela
1.1 Imathiriyeli yovavanyo
Beijing Sunpu Biochem.& neTekhnoloji.Co., Ltd. kufuneka anike i-diludine;kwaye isilwanyana esivavanyiweyo siya kubhekisa kwizikhukukazi zaseRoma ezizalelayo ezineentsuku ezingama-300 ubudala.
Ukutya okulingwayo: ukutya kovavanyo lovavanyo kufuneka kulungiswe ngokwemeko yokwenyani ngexesha lokuvelisa ngokwesiseko somgangatho we-NRC, njengoko kuboniswe kwiThebhile 1.
1.2 Indlela yokuvavanya
1.2.1 Umfuniselo wokondla: umfuniselo wesondlo kufuneka uphunyezwe kwifama yeNkampani yaseHongji kwisiXeko saseJiande;I-1024 yamaRoma yokubeka iinkukhu kufuneka zikhethwe kwaye zahlulwe zibe ngamaqela amane ngokungacwangciswanga kwaye ngalinye libe ngamaqhekeza angama-256 (iqela ngalinye kufuneka liphindwe kane, kwaye inkukhu nganye kufuneka iphindwe ngamaxesha angama-64);iinkukhu kufuneka zityiswe ngezidlo ezine ezinemixholo eyahlukeneyo yediludine, kwaye i-0, 100, 150, 200mg/kg yesondlo kufuneka yongezwe kwiqela ngalinye.Uvavanyo lwaqaliswa ngoAprili 10, 1997;kwaye iinkukhu zazinokufumana ukutya kwaye zithabathe amanzi ngesisa.Ukutya okuthatyathwe liqela ngalinye, izinga lokuzala, imveliso yeqanda, iqanda elaphukileyo kunye nenani leqanda elingaqhelekanga kufuneka libhalwe phantsi.Ngaphezu koko, olu vavanyo lwaphela ngoJuni 30, 1997.
1.2.2 Umlinganiselo womgangatho weqanda: Amaqanda angama-20 kufuneka athathwe ngokungacwangciswanga xa uvavanyo luphunyeziwe kwiintsuku ezine ezingama-40 ukuze kulinganiswe izikhombisi ezinxulumene nomgangatho weqanda, njengesalathiso sokumila kweqanda, iyunithi yehaugh, ubunzima beqokobhe, Ubukhulu beqokobhe, isalathisi se-yolk, ubunzima be-yolk, njl. Ngaphezu koko, umxholo we-cholesterol kwi-yolk kufuneka ulinganiswe ngokusebenzisa indlela ye-COD-PAP phambi kwe-Cicheng reagent eveliswa yi-Ningbo Cixi Biochemical Test Plant.
1.2.3 Umlinganiselo wesalathiso se-serum biochemical index: Iinkukhu zovavanyo eziyi-16 kufuneka zithathwe kwiqela ngalinye ngexesha ngalinye xa uvavanyo beluphunyezwa kangangeentsuku ezingama-30 naxa uvavanyo lugqityiwe ukulungiselela i-serum emva kokuthatha isampulu yegazi elisuka kumthambo okwiphiko.Iserum kufuneka igcinwe kubushushu obuphantsi (-20℃) ukuze kulinganiswe izalathisi ze-biochemical ezifanelekileyo.Ipesenti yamafutha esisu kunye nomxholo we-lipid wesibindi kufuneka zilinganiswe emva kokuxhela kunye nokukhupha amanqatha esisu kunye nesibindi xa kugqitywa ukuthatyathwa kwegazi.
I-superoxide dismutase (SOD) kufuneka ilinganiswe ngokusebenzisa indlela yokugcoba phambi kwekiti ye-reagent eveliswa yi-Beijing Huaqing Biochem.& neTekhnoloji.Iziko loPhando.I-uric acid (UN) kwiserum kufuneka ilinganiswe ngokusebenzisa indlela ye-U ricase-PAP phambi kwekiti ye-reagent yeCicheng;i-triglyceride (TG3) kufuneka ilinganiswe ngokusebenzisa i-GPO-PAP indlela yesinyathelo esinye phambi kwekiti ye-reagent yeCicheng;i-lipase kufuneka ilinganiswe ngokusebenzisa i-nephelometry phambi kwekiti ye-reagent ye-Cicheng;i-cholesterol epheleleyo ye-serum (CHL) kufuneka ilinganiswe ngokusebenzisa indlela ye-COD-PAP phambi kwekiti ye-reagent ye-Cicheng;i-glutamic-pyruvic transaminase (GPT) kufuneka ilinganiswe ngokusebenzisa i-colorimetry phambi kwekiti ye-reagent ye-Cicheng;i-glutamic-oxalacetic transaminase (GOT) kufuneka ilinganiswe ngokusebenzisa i-colorimetry phambi kwekiti ye-reagent ye-Cicheng;i-alkaline phosphatase (ALP) kufuneka ilinganiswe ngokusebenzisa indlela yokulinganisa phambi kwekiti yeCicheng reagent;i-calcium ion (Ca2+) kwi-serum kufuneka ilinganiswe ngokusebenzisa indlela ye-methylthymol blue complexone phambi kwekiti ye-Cicheng reagent;i-phosphorus ye-inorganic (P) kufuneka ilinganiswe ngokusebenzisa indlela ye-molybdate eluhlaza okwesibhakabhaka phambi kwekiti ye-reagent ye-Cicheng.
2 Iziphumo zovavanyo
2.1 Isiphumo sokubeka intsebenzo
Imisebenzi yokubeka yamaqela ahlukeneyo aqhutywe ngokusebenzisa i-diludine iboniswe kwiThebhile 2.
Itheyibhile 2 Ukusebenza kweenkukhu ezityiswa nge-basal diet ezongezwa ngamanqanaba amane e-diludine
| Ubungakanani bediludine ekufuneka bongezwe (mg/kg) | ||||
| 0 | 100 | 150 | 200 | |
| Ukuthathwa kokutya (g) |  | |||
| Ireyithi yokubeka (%) | ||||
| Umndilili wobunzima beqanda (g) | ||||
| Umlinganiselo wezinto kwiqanda | ||||
| Umlinganiselo weqanda elophukileyo (%) | ||||
| Umlinganiselo weqanda elingaqhelekanga (%) | ||||
Ukususela kwiThebhile 2, amazinga okubekwa kwawo onke amaqela acutshungulwa ngokusebenzisa i-diludine aphuculwe ngokucacileyo, apho umphumo xa ucutshungulwa ngokusebenzisa i-150mg / kg ilungile (ukuya kwi-83.36%), kunye ne-11.03% (p <0.01) iphuculwe xa kuthelekiswa kunye neqela lereferensi;ngoko ke i-diludine inefuthe lokuphucula izinga lokubeka.Kubonwa kubunzima obuqhelekileyo beqanda, ubunzima beqanda buyanda (p>0.05) ngokunyuka kwe-diludine ekutyeni kwemihla ngemihla.Xa kuthelekiswa neqela lereferensi, umehluko phakathi kwazo zonke iinxalenye ezicutshungulwayo zamaqela acutshungulwa ngokusebenzisa i-200mg / kg ye-diludine ayibonakali xa i-1.79g yokutya ifakwe ngokulinganayo;nangona kunjalo, umahluko uya kubonakala ngokuthe ngcembe kunye nokunyuka kwe-diludine, kwaye umahluko womlinganiselo wezinto kwiqanda phakathi kwamalungu acutshungulwayo uyabonakala (p <0.05), kwaye umphumo ulungile xa i-150mg / kg ye-diludine kwaye 1.25: I-1 eyancitshiswa kwi-10.36% (p <0.01) xa kuthelekiswa neqela lereferensi.Kubonwa ukusuka kwinqanaba leqanda eliphukileyo lazo zonke iindawo ezicutshungulwayo, izinga leqanda eliphukileyo (p<0.05) lingancitshiswa xa i-diludine yongezwa ekudleni kwansuku zonke;kunye nepesenti yamaqanda angaqhelekanga ancitshisiwe (p <0.05) kunye nokunyuka kwe-diludine.
2.2 Impembelelo kumgangatho weqanda
Kubonwa kwiThebhile 3, isalathiso semilo yeqanda kunye neqanda elithile lomxhuzulane alichaphazeleki (p> 0.05) xa i-diludine yongezwa kwisidlo semihla ngemihla, kwaye ubunzima beqokobhe buyanda kunye nokwandisa i-diludine yongezwa kwisidlo semihla ngemihla, apho ubunzima bamaqokobhe bunyuswe kwi-10.58% kunye ne-10.85% (p <0.05) ngokulandelelanayo xa kuthelekiswa namaqela ereferensi xa i-150 kunye ne-200mg / kg ye-diludine yongezwa;Ubukhulu beqokobhe leqanda bunyuswa ngokunyuka kwe-diludine ekutyeni kwemihla ngemihla, apho ubukhulu beqokobhe leqanda bunyuswe nge-13.89% (p<0.05) xa i-100mg/kg ye-diludine idityaniswa xa ithelekiswa namaqela ereferensi, kunye nobukhulu. amaqokobhe eqanda anyuswe nge-19.44% (p<0.01) kunye ne-27.7% (p<0.01) ngokulandelelana xa i-150 kunye ne-200mg/kg zongezwa.Iyunithi ye-Haugh (p <0.01) iphuculwe ngokucacileyo xa i-diludine yongezwa, ebonisa ukuba i-diludine inefuthe lokukhuthaza ukuhlanganiswa kwe-albumen engqindilili yeqanda elimhlophe.I-diludine inomsebenzi wokuphucula isalathisi se-yolk, kodwa umehluko awukho ngokucacileyo (p <0.05).Imixholo ye-cholesterol ye-yolk yeqanda kuwo onke amaqela ahlukeneyo kwaye ingancitshiswa ngokucacileyo (p <0.05) emva kokongeza i-150 kunye ne-200mg / kg ye-diludine.Ubunzima beqanda lomthubi bohlukile omnye komnye ngenxa yezixa ezahlukeneyo zediludine ezidityanisiweyo, apho ubunzima bomthubi weqanda buphuculwa nge-18.01% kunye ne-14.92% (p<0.05) xa i-150mg/kg kunye ne-200mg/kg ithelekiswa. kunye neqela lereferensi;ngoko ke, i-diludine efanelekileyo inefuthe lokukhuthaza ukuhlanganiswa kweqanda leqanda.
Uluhlu lwesi-3 Iziphumo ze-diludine kumgangatho weqanda
| Ubungakanani bediludine ekufuneka bongezwe (mg/kg) | ||||
| Umgangatho weqanda | 0 | 100 | 150 | 200 |
| Isalathiso sokumila kweqanda (%) |  | |||
| Umxhuzulane okhethekileyo weqanda (g/cm3) | ||||
| Ubunzima obunxulumene neqokobhe leqanda (%) | ||||
| Ukutyeba kweqokobhe leqanda (mm) | ||||
| Iyunithi yeHaugh (U) | ||||
| Isalathisi seqanda (%) | ||||
| ICholesterol yeqanda lomthubi (%) | ||||
| Ubunzima obunxulumene neqanda lomthubi (%) | ||||
2.3 Iimpembelelo kwipesenti yamafutha esisu kunye nomxholo wamafutha esibindi kwinkukhu ezizalelayo
Jonga umzobo 1 kunye noMzobo 2 ngeziphumo ze-diludine kwipesenti yamafutha esiswini kunye nomxholo wamafutha esibindi senkukhu ezizalelayo.
Umfanekiso woku-1 Isiphumo se-diludine kwipesenti yamanqatha e-bodominal (PAF) ezikhukukazi ezizalelayo
| Ipesenti yamafutha e-bodominal | |
| Umthamo wediludine oza kongezwa |
Umfanekiso we-2 Impembelelo ye-diludine kumxholo wamafutha esibindi (LF) wenkukhu ezizalelayo
| Umxholo wamafutha esibindi | |
| Umthamo wediludine oza kongezwa |
Kubonwa kwi-Figure 1, iipesenti zamanqatha e-bodominal yeqela lokuvavanya ziyancipha kwi-8.3% kunye ne-12.11% (p <0.05) ngokulandelanayo xa i-100 kunye ne-150mg / kg ye-diludine xa kuthelekiswa neqela lereferensi, kwaye ipesenti yamafutha e-bodominal iyancitshiswa. kwi-33.49% (p <0.01) xa i-200mg / kg ye-diludine yongezwa.Kubonwa kwi-Figure 2, iziqulatho zesibindi sesibindi (zomile ngokupheleleyo) ezicutshungulwe yi-100, 150, 200mg / kg ye-diludine ngokulandelanayo ziyancipha kwi-15.00% (p <0.05), 15.62% (p <0.05) kunye ne-27.7% (p< 0.01) ngokulandelelanayo xa kuthelekiswa neqela lereferensi;ngoko ke, i-diludine inefuthe lokunciphisa ipesenti yamafutha e-bodominal kunye nomxholo wesibindi wesibindi somxholo wokubeka ngokucacileyo, apho umphumo ulungile xa i-200mg / kg ye-diludine yongezwa.
2.4 Impembelelo kwi-serum biochemical index
Kubonwa kwiThebhile 4, umahluko phakathi kwamacandelo aqhutywe ngexesha leSigaba I (30d) sovavanyo lwe-SOD alubonakali, kwaye i-serum biochemical indexes yawo onke amaqela apho i-diludine yongezwa kwiSigaba II (80d) sovavanyo luphezulu. kuneqela lereferensi (p <0.05).I-uric acid (p<0.05) kwi-serum inokuncitshiswa xa i-150mg/kg kunye ne-200mg/kg ye-diludine yongezwa;ngelixa umphumo (p <0.05) ifumaneka xa i-100mg / kg ye-diludine yongezwa kwiSigaba I. I-diludine inokunciphisa i-triglyceride kwi-serum, apho umphumo ulungile (p <0.01) kwiqela xa i-150mg / kg I-diludine yongezwa kwiSigaba soku-I, kwaye iyona nto ifanelekileyo kwiqela xa i-200mg / kg ye-diludine yongezwa kwiSigaba sesi-II.I-cholesterol iyonke kwi-serum iyancipha kunye nokunyuka kwe-diludine eyongeziweyo kwisidlo semihla ngemihla, ngakumbi imixholo ye-cholesterol epheleleyo kwi-serum iyancipha i-36.36% (p <0.01) kunye ne-40.74% (p <0.01) ngokulandelelana xa i-150mg / kg kunye ne-200mg / kg ye-diludine yongezwa kwiSigaba soku-I xa kuthelekiswa neqela lokubhekisela, kwaye iyancipha i-26.60% (p <0.01), 37.40% (p <0.01) kunye ne-46.66% (p <0.01) ngokulandelanayo xa i-100mg / kg, i-150mg / kg kunye ne-200mg / kg ye-diludine yongezwa kwiSigaba sesi-II xa kuthelekiswa neqela lokubhekisela.Ngaphezu koko, i-ALP inyuswe kunye nokunyuka kwe-diludine eyongeziweyo ekudleni kwansuku zonke, ngelixa ixabiso le-ALP kwiqela apho i-150mg / kg kunye ne-200mg / kg ye-diludine yongezwa iphezulu kuneqela lereferensi (p <0.05) ngokucacileyo.
Itheyibhile 4 Iziphumo ze-diludine kwiiparamitha ze-serum
| Ubungakanani bediludine ekufuneka bongezwe (mg/kg) kwiSigaba soku-I (30d) sovavanyo | ||||
| Into | 0 | 100 | 150 | 200 |
| I-Superoxide dismutase (mg/mL) |  | |||
| Uric acid | ||||
| I-Triglyceride (mmol/L) | ||||
| I-Lipase (U/L) | ||||
| ICholesterol (mg/dL) | ||||
| I-Glutamic-pyruvic transaminase (U/L) | ||||
| I-Glutamic-oxalacetic transaminase (U/L) | ||||
| Ialkaline phosphatase (mmol/L) | ||||
| I-calcium ion (mmol/L) | ||||
| Inorganic phosphorus (mg/dL) | ||||
| Isixa se-diludine kufuneka sifakwe (mg/kg) kwiSigaba sesi-II (80d) sovavanyo | ||||
| Into | 0 | 100 | 150 | 200 |
| I-Superoxide dismutase (mg/mL) |  | |||
| Uric acid | ||||
| I-Triglyceride (mmol/L) | ||||
| I-Lipase (U/L) | ||||
| ICholesterol (mg/dL) | ||||
| I-Glutamic-pyruvic transaminase (U/L) | ||||
| I-Glutamic-oxalacetic transaminase (U/L) | ||||
| Ialkaline phosphatase (mmol/L) | ||||
| I-calcium ion (mmol/L) | ||||
| Inorganic phosphorus (mg/dL) | ||||
3 Uhlalutyo nengxoxo
3.1 I-diludine kuvavanyo iphucule izinga lokubeka, ubunzima beqanda, iyunithi ye-Haugh kunye nobunzima obunxulumene neqanda leqanda, elibonisa ukuba i-diludine ineempembelelo zokukhuthaza ukuxutywa kweprotheni kunye nokuphucula inani le-synthesis ye-egg. albumen yeqanda elimhlophe kunye neprotheni yeqanda lomthubi.Ngaphezu koko, umxholo we-uric acid kwi-serum wancitshiswa ngokucacileyo;kwaye kwavunywa ngokubanzi ukuba ukuncitshiswa komxholo we-nitrogen engeyiyo iprotheni kwi-serum kwakuthetha ukuba isantya se-catabolism seprotheni sancitshiswa, kwaye ixesha lokugcinwa kwe-nitrogen lihlehlisiwe.Esi siphumo sinikeze isiseko sokunyusa ukugcinwa kweprotheyini, ukukhuthaza ukubeka amaqanda kunye nokuphucula ubunzima beqanda leenkukhu ezibeka amaqanda.Isiphumo sovavanyo sibonise ukuba impembelelo yokubeka ilungile xa i-150mg / kg ye-diludine yongezwa, eyona nto yayihambelana nesiphumo.[6,7]ye-Bao Erqing kunye neQin Shangzhi kwaye ifunyenwe ngokudibanisa i-diludine ngexesha lokugqibela lokubeka iinkukhu.Isiphumo sancitshiswa xa isixa se-diludine sidlula i-150mg/kg, enokuba ngenxa yokuguqulwa kweprotheyini.[8]yachaphazeleka ngenxa yomthamo ogqithisileyo kunye nomthwalo ogqithisileyo wemetabolism yelungu kwi-diludine.
3.2 Uxinzelelo lweCa2+kwi-serum yeqanda lokubeka yancitshiswa, i-P kwi-serum yancitshiswa ekuqaleni kwaye umsebenzi we-ALP wanda ngokucacileyo phambi kwe-diludine, ebonisa ukuba i-diludine ichaphazele i-metabolism ye-Ca kunye ne-P ngokucacileyo.U-Yue Wenbin uxele ukuba i-diludine inokukhuthaza ukufunxa[9] izinto ezimbiwa phantsi iFe kunye neZn;I-ALP yayikhona ikakhulu kwizihlunu, ezifana nesibindi, ithambo, umgudu wamathumbu, intso, njl.;I-ALP kwi-serum yayisuka kwisibindi kunye nethambo ubukhulu becala;I-ALP emathanjeni yayikhona kwi-osteoblast ikakhulu kwaye inokudibanisa i-phosphate ion kunye ne-Ca2 ukusuka kwi-serum emva kokuguqulwa ngokukhuthaza ukubola kwe-phosphate kunye nokwandisa ukuxinwa kwe-phosphate ion, kwaye yafakwa kwithambo ngendlela ye-hydroxyapatite, njl. . ukuze kukhokelele ekuncitshisweni kwe-Ca kunye ne-P kwiserum, ehambelana nokunyuka kobukhulu beqokobhe leqanda kunye nobunzima beqokobhe leqanda kwizalathi zomgangatho weqanda.Ngaphezu koko, isantya seqanda esaphukileyo kunye nepesenti yeqanda elingaqhelekanga lancitshiswa ngokucacileyo ngokubhekiselele ekusebenzeni kokubeka, okukwachaza le ngongoma.
I-3.3 Ukufakwa kwamafutha esiswini kunye nomxholo wesibindi wesibindi sezikhukhukazi ezibeka amaqanda zancitshiswa ngokucacileyo ngokufaka i-diludine ekudleni, okuye kwabonisa ukuba i-diludine inefuthe lokuthintela ukuhlanganiswa kwamafutha emzimbeni.Ukongezelela, i-diludine inokuphucula umsebenzi we-lipase kwi-serum kwinqanaba lokuqala;umsebenzi we-lipase wanda ngokucacileyo kwiqela apho i-100mg / kg ye-diludine yongezwa, kwaye umxholo we-triglyceride kunye ne-cholesterol kwi-serum yancitshiswa (p <0.01), ebonisa ukuba i-diludine inokukhuthaza ukubola kwe-triglyceride. kunye nokuthintela ukuhlanganiswa kwe-cholesterol.Ukubekwa kwamafutha kunokuthintelwa ngenxa yokuba i-enzyme ye-lipid metabolism esibindini[10,11], kunye nokunciphisa i-cholesterol kwi-yolk yeqanda nayo yachaza le ngongoma [13].U-Chen Jufang wabika ukuba i-diludine inokuthintela ukubunjwa kwamanqatha kwisilwanyana kwaye iphucule ipesenti yenyama enqabileyo ye-broilers kunye nehagu, kwaye ibe nefuthe lokunyanga isibindi esinamafutha.Isiphumo sovavanyo sacacisa le ndlela yokusebenza, kwaye iziphumo zokuqhawula kunye nokuqwalaselwa kweenkukhu zovavanyo zibonise ukuba i-diludine inokunciphisa ukwenzeka kwesibindi esinamafutha kwiinkukhu ezizalelayo ngokucacileyo.
3.4 I-GPT kunye ne-GOT zizalathisi ezibini ezibalulekileyo ezibonisa imisebenzi yesibindi nentliziyo, kwaye isibindi nentliziyo zinokonakala ukuba imisebenzi yazo iphezulu kakhulu.Imisebenzi ye-GPT kunye ne-GOT kwi-serum ayizange iguqulwe ngokucacileyo xa i-diludine yongezwa kuvavanyo, ebonisa ukuba isibindi kunye nentliziyo azizange zonakaliswe;ukuqhubela phambili, umphumo wokulinganisa we-SOD ubonise ukuba umsebenzi we-SOD kwi-serum unokuphuculwa ngokucacileyo xa i-diludine isetyenziswe ixesha elithile.I-SOD ibhekisela kwi-scavenger enkulu ye-superoxide free radical emzimbeni;kubaluleke kakhulu ukugcina ingqibelelo ye-membrane yebhayoloji, ukuphucula amandla okuzivikela omzimba kunye nokugcina impilo yesilwanyana xa umxholo we-SOD emzimbeni usanda.U-Quh Hai, njl. ingxelo yokuba i-diludine inokuphucula umsebenzi we-6-glucose phosphate dehydrogenase kwi-membrane ye-biological kwaye izinzile izicubu [2] zeseli ye-biological.U-Sniedze ubonise ukuba i-diludine ithintele umsebenzi [4] we-NADPH cytochrome C reductase ngokucacileyo emva kokufunda ubudlelwane phakathi kwe-diludine kunye ne-enzyme efanelekileyo kwi-NADPH ikhonkco yokudlulisa i-electron kwi-microsome yesibindi segundane.I-Odydents nayo yabonisa ukuba i-diludine yayinxulumene [4] nenkqubo ye-oxidase edibeneyo kunye ne-microsomal enzyme ehambelana ne-NADPH;kunye nendlela yesenzo se-diludine emva kokungena kwisilwanyana kukudlala indima yokumelana ne-oxidation kunye nokukhusela i-membrane ye-biological [8] ngokuthintela umsebenzi we-electron trans transfer NADPH enzyme ye-microsome kunye nokuthintela inkqubo ye-peroxidation ye-lipid compound.Iziphumo zovavanyo zibonise ukuba umsebenzi wokukhusela we-diludine kwi-membrane yebhayoloji ukusuka kutshintsho lomsebenzi we-SOD ukuya kutshintsho lwemisebenzi ye-GPT kunye ne-GOT kwaye yangqina iziphumo zokufunda ze-Sniedze kunye ne-Odydents.
Isalathiso
1 uZhou Kai, uZhou Mingjie, uQin Zhongzhi, njl. njl. Uphononongo malunga ne-diludine yokuphucula ukusebenza kweegushaJ. Ingca kunyeLivestock 1994 (2): 16-17
I-2 Qu Hai, Lv Ye, Wang Baosheng, Umphumo we-diludine wongezwa ekudleni kwansuku zonke kwizinga lokukhulelwa kunye nomgangatho wesidoda wenyama yomvundla.J. Ijenali yesiTshayina yokuLinywa kwemivundla1994(6): 6-7
3 Chen Jufang, Yin Yuejin, Liu Wanhan, njl. Uvavanyo lwesicelo esandisiweyo se-diludine njengesongezo sokutya.Uphando lokutyisa1993 (3): 2-4
4 Zheng Xiaozhong, Li Kelu, Yue Wenbin, njl.njl. Ingxoxo ngesiphumo sesicelo kunye nendlela yesenzo se-diludine njengomkhuthazi wokukhula kweenkukhu.Uphando lokutyisa1995 (7): 12-13
5 Chen Jufang, Yin Yuejin, Liu Wanhan, njl. Uvavanyo lwesicelo esandisiweyo se-diludine njengesongezo sokutya.Uphando lokutyisa1993 (3): 2-5
6 Bao Erqing, Gao Baohua, Uvavanyo lwediludine yokondla inzala yasePekingUphando lokutyisa1992 (7): 7-8
7 Qin Shangzhi Uvavanyo lokuphucula imveliso yeenkukhu zenyama ngexesha lokugqibela lokubeka ngokusebenzisa i-diludineIjenali yeGuangxi yokufuya iziLwanyana kunye noNyango lweZilwanyana1993.9(2): 26-27
8 Dibner J Jl Lvey FJ Iprotheyini yeHepatic kunye ne-amino acid yemetabolian kwiinkukhu Iinkukhu Sci1990.69(7): 1188- 1194
I-9 Yue Wenbin, uZhang Jianhong, uZhao Peie, njl. njlUkutya & nemfuyo1997, 18(7): 29-30
10 Mildner A na M, Steven D Clarke Porcine fatty acid synthase cloning ye-DNA encedisayo, ukuhanjiswa kwezicubu ze-itsmRNA kunye nokunyanzeliswa kwentetho ye-somatotropin kunye neprotheni yokutya uJ Nutri 1991, 121 900
11 W alzon RL Smon C, M orishita T, et a I Fatty liver hemorrhagic syndrome kwizikhukukazi ezigqithise ukutya okuhlanjululwayo Imisebenzi ekhethiweyo ye-enzyme kunye ne-histology yesibindi ngokumalunga nokuhlonishwa kwesibindi kunye nokusebenza kokuzala.Iinkukhu zeSayensi,1993 72(8): 1479- 1491
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Ixesha lokuposa: Jun-07-2021