I mea e loiloi ai i ka hopena o ka hoʻohui ʻana i ka triglyceride i ka meaʻai ma ka hana ʻana i ka protein microbial rumen a me nā ʻano fermentation o nā hipa liʻiliʻi liʻiliʻi liʻiliʻi, ua hana ʻia ʻelua mau hoʻokolohua in vitro a in vivo.

ʻO ka hoʻāʻo in vitro: ua hoʻohana ʻia ka meaʻai basal (ma muli o ka mea maloʻo) me ka triglyceride o 0, 2, 4, 6 a me 8g / kg i mea hoʻohui, a ua hoʻohui ʻia ka wai rumen o nā hipa liʻiliʻi liʻiliʻi liʻiliʻi, a hoʻomoʻa ʻia ma 39 ℃ no 48h in vitro.

I ka ho'āʻo vivo: 45 mau hipa kāne makua i māhele ʻia i 5 pūʻulu e like me ko lākou kaumaha mua (55 ± 5 kg).ʻO Glyceryl tributylateo 0, 2, 4, 6 a me 8 g/kg (ma muli o ka mea maloʻo) ua hoʻohui ʻia i ka meaʻai kumu, a ua hōʻiliʻili ʻia ka wai rumen a me ka mimi no nā lā 18.

Ka hopena hoao

1).Ka hopena i ka waiwai pH a me ka hoʻokaʻawale ʻana o ka waika momona

Ua hōʻike ʻia nā hopena i ka emi ʻana o ka pH waiwai o ka moʻomeheu moʻomeheu a me ka hoʻonui ʻana o ka nui o nā ʻakika momona volatile (TVFA), acetic acid, butyric acid a me branched chain volatile fatty acids (BCVFA) i hoʻonui ʻia i ka wā.tributyl glycerideua hoʻohui ʻia i ka substrate. Ua hōʻike ʻia nā hopena o ka hoʻāʻo in vivo i ka emi ʻana o ka mea maloʻo (DMI) a me ka waiwai pH, a me ka hoʻonui ʻana o ka TVFA, acetic acid, propionic acid, butyric acid a me BCVFA me ka hoʻohui ʻana otributyl glycerideUa hōʻike ʻia nā hopena o ka hoʻāʻo in vivo i ka emi ʻana o ka waiwai maloʻo (DMI) a me ka pH waiwai, a me ka hoʻonui ʻana o ka TVFA, acetic acid, propionic acid, butyric acid a me BCVFA me ka hoʻohui ʻana i ka tributyl glyceride.

Ua hōʻike ʻia nā hopena o ka hoʻāʻo in vivo i ka emi ʻana o ka waiwai maloʻo (DMI) a me ka waiwai pH, a me ka hoʻonui ʻana o ka TVFA, acetic acid, propionic acid, butyric acid a me BCVFA me ka hoʻohui ʻana otributyl glyceride.

2).E hoʻomaikaʻi i ka helu degradation o nā meaʻai

Ua hoʻonui ʻia ka helu degradation o DM, CP, NDF a me ADF i ka wātributyl glycerideua hoʻohui ʻia i ka substrate in vitro.

3).Hoʻonui i ka hana o ka cellulose degrading enzyme

ʻO ka hoʻohui ʻana otributyrinin vitro linearly hoʻonui i nā hana o xylanase, carboxymethyl cellulase a me microcrystalline cellulase.Ua hōʻike ʻia nā hoʻokolohua vivo ua hoʻonui ka triglyceride linearly i nā hana o xylanase a me carboxymethyl cellulase.

4).E hoʻomaikaʻi i ka hana microbial protein

Ua hōʻike ʻia nā hōʻike vivotributyrinlinearly hoʻonui i ka nui o kēlā me kēia lā o allantoin, uric acid a komo i ka purine microbial i loko o ka mimi, a hoʻonui i ka synthesis o ka rumen microbial nitrogen.

Ka hopena

Tributyrinhoʻomaikaʻi i ka synthesis o ka rumen microbial protein, ka ʻike o ka nui o nā momona momona a me ka hana o ka cellulose degrading enzymes, a hoʻoikaika i ka hoʻohaʻahaʻa a me ka hoʻohana ʻana i ka mea maloʻo, protein crude, neutral detergent fiber and acid detergent fiber i ka meaʻai.

Hōʻike ia he hopena maikaʻi ka triglyceride i ka hua a me ka fermentation o ka protein microbial rumen, a he hopena maikaʻi paha i ka hana hana o nā hipa wahine makua.