Ukuze uvavanye isiphumo sokongeza i-triglyceride ekutyeni kwimveliso yeprotein ye-rumen microbial kunye neempawu zokuvundiswa kweemazi ezincinci ezinomsila omdala, imifuniselo emibini yenziwa kwi-vitro nakwi-vivo.

Uvavanyo lwe-in vitro: ukutya kwe-basal (okusekelwe kumbandela owomileyo) kunye noxinzelelo lwe-triglyceride ye-0, 2, 4, 6 kunye ne-8g / kg isetyenziswe njenge-substrate, kunye nejusi yerumen yeemazi zeemazi eziNcinane eziNcinane eziNcinane zongezwa, kwaye zafukanyelwa kwi-39. ℃ ye-48h ye-in vitro.

Uvavanyo lwe-invivo: iimazi ezikhulileyo ezingama-45 zahlulwa ngokungenamkhethe zangamaqela ama-5 ngokobunzima bazo bokuqala (55 ± 5 kg).I-Glyceryl tributylateye-0, 2, 4, 6 kunye ne-8 g / kg (esekelwe kwizinto ezomileyo) zongezwa kwisondlo esisisiseko, kwaye i-rumen fluid kunye nomchamo zaqokelelwa iintsuku ezili-18.

Iziphumo zovavanyo

1).Impembelelo kwixabiso le-pH kunye ne-volatile fatty acid concentration

Iziphumo zibonise ukuba ixabiso le-pH yenkcubeko yenkcubeko lehlile ngokulandelelana kwaye ukugxininiswa kwe-fatty volatile fatty acids (TVFA), i-acetic acid, i-butyric acid kunye ne-branched chain volatile fatty acids (BCVFA) yanda ngokulandelelana.i-tributyl glycerideIziphumo zovavanyo lwe-vivo zibonise ukuba i-dry matter intake (DMI) kunye nexabiso le-pH liyancipha, kwaye i-concentrations ye-TVFA, i-acetic acid, i-propionic acid, i-butyric acid kunye ne-BCVFA yanda ngokuhambelana nokongezwa kwe-substrate.i-tributyl glyceride.Iziphumo zovavanyo lwe-vivo zibonise ukuba i-dry matter intake (DMI) kunye nexabiso le-pH liyehla, kwaye ukugxininiswa kwe-TVFA, i-acetic acid, i-propionic acid, i-butyric acid kunye ne-BCVFA yanda ngokuhambelana nokongezwa kwe-tributyl glyceride.

Iziphumo zovavanyo lwe-vivo zibonise ukuba i-dry matter intake (DMI) kunye nexabiso le-pH lehlile, kwaye ukugxila kwe-TVFA, i-acetic acid, i-propionic acid, i-butyric acid kunye ne-BCVFA yonyuka ngokulandelelanai-tributyl glyceride.

2).Ukuphucula izinga lokuthotywa kwezondlo

Izinga elibonakalayo lokuthotywa kwe-DM, CP, NDF kunye ne-ADF linyuke ngokulandelelana xai-tributyl glycerideyongezwa kwi-substrate ye-vitro.

3).Ukuphucula umsebenzi we-cellulose ethobisa i-enzyme

Ukongezwa kwei-tributyrinI-in vitro inyuse ngokulandelelana imisebenzi ye-xylanase, i-carboxymethyl cellulase kunye ne-microcrystalline cellulase.Uvavanyo lwe-vivo lubonise ukuba i-triglyceride inyuse ngokulandelelana imisebenzi ye-xylanase kunye ne-carboxymethyl cellulase.

4).Ukuphucula imveliso yeprotheyini ye-microbial

Iimvavanyo ze-vivo zibonise ukubai-tributyrinngokomgca yandise umthamo wemihla ngemihla we-allantoin, i-uric acid kunye ne-microbial purine emchameni, kwaye yandisa ukuhlanganiswa kwe-rumen microbial nitrogen.

Ukuqukumbela

I-Tributyriniphucule ukuhlanganiswa kweprotein ye-rumen microbial, umxholo we-fatty volatile acids iyonke kunye nomsebenzi we-cellulose ethobisa i-enzymes, kunye nokukhuthaza ukuthotywa kunye nokusetyenziswa kwezinto ezomileyo, i-protein ekrwada, i-fiber engathathi hlangothi kunye ne-acid detergent fiber ekutyeni.

Ibonisa ukuba i-triglyceride inefuthe elihle kwisivuno kunye nokuvundiswa kweprotein ye-rumen microbial, kwaye ingaba neempembelelo ezilungileyo kwintsebenzo yemveliso yeemazi ezindala.