Mu rwego rwo gusuzuma ingaruka zo kongeramo triglyceride mu ndyo y’umusemburo wa poroteyine ya rumen na fermentation biranga intama ntoya zikuze, hakozwe ubushakashatsi bubiri muri vitro no muri vivo

Mu isuzuma rya vitro: indyo yibanze (ishingiye ku bintu byumye) hamwe na triglyceride ya 0, 2, 4, 6 na 8g / kg yakoreshejwe nka substrate, hanyuma umutobe wa rumen w’intama ntoya ukuze wongeyeho, hanyuma ushyirwa kuri 39 ℃ kuri 48h muri vitro.

Mu kizamini cya vivo: Intama 45 zikuze zagabanijwe mu matsinda 5 ukurikije uburemere bwazo (55 ± 5 kg).Glyceryl tributylateya 0, 2, 4, 6 na 8 g / kg (hashingiwe ku bintu byumye) byongewe ku ndyo y’ibanze, maze amazi ya rumen n’inkari byegeranijwe iminsi 18.

Ibisubizo by'ibizamini

1).Ingaruka ku gaciro ka pH hamwe na aside irike ihindagurika

Ibisubizo byagaragaje ko agaciro ka pH k'umuco gaciriritse kagabanutse ku buryo bugaragara kandi ubunini bwa acide fatty acide (TVFA), acide acetike, butyric aside hamwe nuruhererekane rw'amashami ya volatile fatty acide (BCVFA) byiyongereye cyane iyotributyl glycerideyongerewe kuri substrate.Ibisubizo bya test ya vivo byerekanaga ko gufata ibintu byumye (DMI) nagaciro ka pH byagabanutse, kandi kwibanda kuri TVFA, acide acetike, aside protionique, aside butyric na BCVFA byiyongereye kumurongo hiyongereyehotributyl glyceride.Ibisubizo by'ibizamini bya vivo byerekanaga ko gufata ibintu byumye (DMI) n'agaciro ka pH byagabanutse, kandi ubukana bwa TVFA, acide acetike, aside protionique, aside butyric na BCVFA byiyongereye ku murongo hiyongereyeho tributyl glyceride.

Ibisubizo by'ibizamini bya vivo byerekanye ko gufata ibintu byumye (DMI) n'agaciro ka pH byagabanutse, kandi kwibanda kuri TVFA, aside acike, aside protionique, aside butyric na BCVFA byiyongereye ku murongo hiyongereyehotributyl glyceride.

2).Kunoza igipimo cyo kwangirika kwintungamubiri

Igipimo kigaragara cyo gutesha agaciro DM, CP, NDF na ADF cyiyongereye kumurongo iyotributyl glycerideyariyongereye kuri substrate muri vitro.

3).Kunoza ibikorwa bya selile yangiza selile

Inyongera yatributyrinmuri vitro yongereye umurongo ibikorwa bya xylanase, carboxymethyl selulase na microcrystalline selile.Mu bushakashatsi bwa vivo bwerekanye ko triglyceride yongereye umurongo ibikorwa bya xylanase na carboxymethyl selile.

4).Kunoza umusaruro wa mikorobe

Muri vivo ibizamini byerekanaga kotributyrinbyiyongereye ku buryo bugaragara urugero rwa buri munsi rwa allantoin, acide uric hamwe na mikorobe ya purine mu nkari, kandi byongera synthesis ya azote ya rumen.

Umwanzuro

Tributyrinyazamuye synthesis ya proteine ​​ya rumen, ibirimo aside irike yuzuye hamwe nigikorwa cya selile yangiza selile, kandi iteza imbere kwangirika no gukoresha ibintu byumye, poroteyine itavanze, fibre detergent fibre na fibre detergent fibre mumirire.

Irerekana ko triglyceride igira ingaruka nziza ku musaruro no gusembura poroteyine ya rumen mikorobe, kandi ishobora kugira ingaruka nziza ku musaruro w’intama zikuze.