Pofuna kuyesa zotsatira za kuwonjezera triglyceride pazakudya pakupanga mapuloteni amtundu wa rumen ndi mawonekedwe a nayonso mphamvu ya nkhosa zazikazi zazing'ono zamchira, kuyesa kuwiri kunachitika mu vitro ndi mu vivo.

Mayeso a in vitro: zakudya zoyambira (zotengera zowuma) zokhala ndi kuchuluka kwa triglyceride za 0, 2, 4, 6 ndi 8g / kg zidagwiritsidwa ntchito ngati gawo lapansi, ndipo madzi a rumen a nkhosa zazikazi zazikulu zazing'ono zazing'ono adawonjezedwa, ndikuyikidwa pa 39. ℃ kwa 48h mu vitro.

Mayeso a vivo: Nkhosa zazikulu 45 zidagawidwa mwachisawawa m'magulu asanu molingana ndi kulemera kwawo koyamba (55 ± 5 kg).Glyceryl tributylatewa 0, 2, 4, 6 ndi 8 g/kg (zotengera youma) anawonjezeredwa ku zakudya zofunika, ndi rumen madzimadzi ndi mkodzo anasonkhanitsidwa kwa masiku 18.

Zotsatira za mayeso

1).Mphamvu pa pH yamtengo wapatali komanso kusakhazikika kwamafuta acid

Zotsatira zake zidawonetsa kuti pH ya sing'anga ya chikhalidwe idatsika motsatana ndipo kuchuluka kwa mafuta ochulukirapo (TVFA), acetic acid, butyric acid ndi nthambi za chain volatile fatty acids (BCVFA) zidachulukira motsatira.tributyl glycerideZotsatira za mayeso a mu vivo zidawonetsa kuti kudya kowuma (DMI) ndi pH mtengo kudachepa, ndipo kuchuluka kwa TVFA, acetic acid, propionic acid, butyric acid ndi BCVFA kuchulukirachulukira ndikuwonjezeratributyl glyceride.Zotsatira za mayeso a mu vivo zimasonyeza kuti kudya kowuma (DMI) ndi pH mtengo kunachepa, ndipo kuchuluka kwa TVFA, acetic acid, propionic acid, butyric acid ndi BCVFA kumawonjezeka mofanana ndi kuwonjezera kwa tributyl glyceride.

Zotsatira za mayeso a mu vivo zidawonetsa kuti kuchuluka kwa zinthu zowuma (DMI) ndi pH yatsika, ndipo kuchuluka kwa TVFA, acetic acid, propionic acid, butyric acid ndi BCVFA kudakwera motsatana ndikuwonjezeratributyl glyceride.

2).Kupititsa patsogolo kuwonongeka kwa zakudya

Chiwopsezo chowoneka bwino cha DM, CP, NDF ndi ADF chinakwera kwambiri pomwetributyl glycerideadawonjezeredwa ku gawo lapansi mu vitro.

3).Kupititsa patsogolo ntchito ya cellulose degrading enzyme

Kuwonjezera kwatributyrinmu vitro motsatana adawonjezera ntchito za xylanase, carboxymethyl cellulase ndi cellcrystalline cellulase.Kuyesa kwa vivo kunawonetsa kuti triglyceride idakulitsa ntchito za xylanase ndi carboxymethyl cellulase.

4).Kupititsa patsogolo kupanga mapuloteni a microbial

Mayeso a vivo adawonetsa izitributyrinlinearly kuchuluka tsiku ndi tsiku allantoin, uric acid ndi odzipereka tizilombo purine mu mkodzo, ndipo anawonjezera synthesis rumen tizilombo nayitrogeni.

Mapeto

TributyrinKupititsa patsogolo kaphatikizidwe ka mapuloteni a rumen, omwe ali ndi mafuta osasunthika komanso momwe ma cellulose amawonongera michere, komanso kulimbikitsa kuwonongeka ndi kugwiritsa ntchito zinthu zouma, zomanga thupi, zotsukira zopanda ndale komanso zotsukira asidi m'zakudya.

Zimasonyeza kuti triglyceride imakhala ndi zotsatira zabwino pa zokolola ndi nayonso mphamvu ya mapuloteni a rumen microbial, ndipo ikhoza kukhala ndi zotsatira zabwino pakupanga kwa nkhosa zazikulu.