Ukuze uhlole umphumela wokwengeza i-triglyceride ekudleni ekukhiqizeni amaprotheni e-rumen microbial kanye nezici zokuvutshelwa kwezinsikazi ezindala ezinomsila, ukuhlolwa okubili kwenziwa ku-vitro kanye ne-vivo.

Ukuhlolwa kwe-in vitro: ukudla okuyisisekelo (okusekelwe endabeni eyomile) okugxilwe kwe-triglyceride engu-0, 2, 4, 6 kanye no-8g / kg kwasetshenziswa njenge-substrate, kanye nejusi ye-rumen yezimvukazi Ezikhulile Eziyizinsika Ezincane, futhi zafakwa ku-39. ℃ amahora angama-48 e-in vitro.

Ukuhlolwa kwe-vivo: izimvukazi ezindala ezingama-45 zahlukaniswa ngokungahleliwe zaba amaqembu ama-5 ngokwesisindo sazo sokuqala (55 ± 5 kg).I-Glyceryl tributylateka-0, 2, 4, 6 kanye no-8 g/kg (ngokusekelwe endabeni eyomile) yengezwa ekudleni okuyisisekelo, futhi uketshezi lwe-rumen nomchamo kwaqoqwa izinsuku ezingu-18.

Umphumela Wokuhlola

1).Umthelela enanini le-pH kanye nokugxila kwe-fatty acid eguqukayo

Imiphumela ibonise ukuthi inani le-pH le-culture medium lehle ngokulandelana futhi ukugxila kwengqikithi yama-volatile fatty acids (TVFA), i-acetic acid, i-butyric acid kanye ne-branched chain volatile fatty acids (BCVFA) kukhuphuke ngokulinganayo lapho.i-tributyl glycerideImiphumela yokuhlolwa kwe-in vivo ibonise ukuthi ukuthathwa kwento eyomile (DMI) kanye nenani le-pH lehlile, futhi ukugxila kwe-TVFA, i-acetic acid, i-propionic acid, i-butyric acid ne-BCVFA kukhuphuke ngokuhambisana nokwengezwai-tributyl glyceride.Imiphumela yokuhlolwa kwe-in vivo ibonise ukuthi ukuthathwa kwento eyomile (i-DMI) nenani le-pH lehlile, futhi ukugxila kwe-TVFA, i-acetic acid, i-propionic acid, i-butyric acid ne-BCVFA kukhuphuke ngokuhambisana nokwengezwa kwe-tributyl glyceride.

Imiphumela yokuhlolwa kwe-in vivo ibonise ukuthi ukuthathwa kwento eyomile (DMI) kanye nenani le-pH lehlile, futhi ukugxila kwe-TVFA, i-acetic acid, i-propionic acid, i-butyric acid ne-BCVFA kukhuphuke ngokuhambisana nokwengezwai-tributyl glyceride.

2).Thuthukisa izinga lokuwohloka kwezakhamzimba

Izinga elibonakalayo lokuwohloka kwe-DM, CP, NDF kanye ne-ADF lenyuke ngokulinganayo laphoi-tributyl glycerideyengezwe ku-substrate in vitro.

3).Thuthukisa umsebenzi we-cellulose degrading enzyme

Ukwengezwa kwei-tributyrinI-in vitro ikhulise ngokulinganayo imisebenzi ye-xylanase, i-carboxymethyl cellulase kanye ne-microcrystalline cellulase.Ukuhlolwa kwe-vivo kubonise ukuthi i-triglyceride yandisa ngokulinganayo imisebenzi ye-xylanase ne-carboxymethyl cellulase.

4).Thuthukisa ukukhiqizwa kwamaprotheni e-microbial

Ukuhlolwa kwe-vivo kubonise lokhoi-tributyrinngokwemigqa yandisa inani lansuku zonke le-allantoin, i-uric acid kanye ne-microbial purine emchamweni, futhi yandisa ukuhlanganiswa kwe-rumen microbial nitrogen.

Isiphetho

I-Tributyrinithuthukise ukwakheka kweprotein ye-rumen microbial, okuqukethwe kwengqikithi yamafutha acid aguquguqukayo kanye nomsebenzi wama-enzymes okwehlisa i-cellulose, futhi yakhuthaza ukuwohloka nokusetshenziswa kwento eyomile, amaprotheni aluhlaza, i-neutral detergent fiber kanye ne-acid detergent fiber ekudleni.

Kubonisa ukuthi i-triglyceride inomphumela omuhle ekuvuneni nasekuvundiseni kwephrotheni ye-rumen microbial, futhi ingaba nomthelela omuhle ekusebenzeni kokukhiqizwa kwezinsikazi ezindala.