Kuti uongorore mhedzisiro yekuwedzera triglyceride pakudya pane rumen microbial protein kugadzira uye kuvirisa hunhu hwenhunzvi dzemuswe dzakura, kuyedza kuviri kwakaitwa mu vitro uye mu vivo.

In vitro bvunzo: iyo basal kudya (yakavakirwa pane yakaoma nyaya) ine triglyceride yakadzika ye0, 2, 4, 6 uye 8g / kg yakashandiswa se substrate, uye muto werumen weEkura Diki Makadzi Ewee akawedzerwa, uye akajairwa pamakumi matatu nemapfumbamwe. ℃ ye48h in vitro.

In vivo bvunzo: 45 nhunzvi dzakura dzakakamurwa zvisina tsarukano kuita mapoka mashanu zvichienderana nehuremu hwadzo hwekutanga (55 ± 5 kg).Glyceryl tributylateye0, 2, 4, 6 uye 8 g/kg (zvichienderana nechinhu chakaoma) chakawedzerwa kune kudya kwekutanga, uye rumen fluid uye weti zvakaunganidzwa kwemazuva gumi nemasere.

Test Result

1).Mhedzisiro pane pH kukosha uye inoputika fatty acid concentration

Mhedzisiro yacho yakaratidza kuti pH kukosha kwetsika yepakati yakadzikira mutsetse uye kuwanda kweiyo yakazara volatile fatty acids (TVFA), acetic acid, butyric acid uye branched chain volatile fatty acids (BCVFA) yakawedzera mutsara kana.tributyl glycerideyakawedzerwa kune substrate.Migumisiro ye in vivo test yakaratidza kuti iyo yakaoma kudya (DMI) uye pH kukosha kwakaderera, uye kuwedzera kweTVFA, acetic acid, propionic acid, butyric acid uye BCVFA yakawedzera mutsara nekuwedzera kwetributyl glyceride.Migumisiro ye in vivo test yakaratidza kuti iyo yakaoma yekudya (DMI) uye pH kukosha yakaderera, uye kuwedzera kweTVFA, acetic acid, propionic acid, butyric acid uye BCVFA yakawedzera mutsara nekuwedzera kwe tributyl glyceride.

Mhedzisiro yemuyedzo ye in vivo yakaratidza kuti iyo yakaoma nyaya kudya (DMI) uye pH kukosha kwakadzikira, uye kuwanda kweTVFA, acetic acid, propionic acid, butyric acid uye BCVFA yakawedzera mutsara nekuwedzera kwe.tributyl glyceride.

2).Kuvandudza kuwanda kwekuora kwekudya

Iyo inoratidzika kuderedzwa mwero weDM, CP, NDF uye ADF yakawedzera mutsetse apotributyl glycerideyakawedzerwa kune substrate in vitro.

3).Kuvandudza basa re cellulose inosvibisa enzyme

Kuwedzera kwetributyrinin vitro yakawedzera zviitiko zve xylanase, carboxymethyl cellulase uye microcrystalline cellulase.Mu vivo zviedzo zvakaratidza kuti triglyceride yakawedzera zviitiko zve xylanase uye carboxymethyl cellulase.

4).Kuvandudza microbial protein production

In vivo tests yakaratidza izvozvotributyrinmutsara wakawedzera huwandu hwezuva nezuva hweallantoin, uric acid uye yakanyura microbial purine muweti, uye yakawedzera kuumbwa kwerumen microbial nitrogen.

Mhedziso

Tributyrinyakavandudza kuumbwa kwerumen microbial protein, zviri mukati meiyo yakazara volatile fatty acids uye basa re cellulose inoshatisa enzymes, uye yakasimudzira kuderedzwa uye kushandiswa kwezvinhu zvakaoma, crude protein, neutral detergent fiber uye acid detergent fiber mukudya.

Zvinoratidza kuti triglyceride ine simba rakanaka pagoho uye kuviriswa kweprotein inonzi rumen microbial, uye inogona kuva nemigumisiro yakanaka pakugadzirwa kwenhunzvi dzakura.