Tare da fadada sikelin al'adu da karuwar yawan al'adu, cutar Apostichopus japonicus ya zama mai mahimmanci, wanda ya haifar da mummunar asara ga masana'antar kiwo.Cututtukan Apostichopus japonicus suna haifar da su ne ta hanyar ƙwayoyin cuta, ƙwayoyin cuta da ciliates, daga cikinsu cututtukan fata na rot da Vibrio brilliant ke haifarwa shine mafi muni.Tare da tsananta cutar, bangon jikin Apostichopus japonicus ulcers, yana samar da aibobi masu launin shuɗi da fari, kuma a ƙarshe kai ya narke har ya mutu, yana narkewa cikin ƙwayar hanci kamar colloid.A cikin rigakafin cututtuka da magani na gargajiya, ana amfani da maganin rigakafi da yawa.Amma yin amfani da maganin rigakafi na dogon lokaci ba wai kawai yana da ɓoyayyun haɗarin juriya na ƙwayoyin cuta da ragowar ƙwayoyi ba, har ma yana kawo amincin abinci da gurɓataccen muhalli.Saboda haka, ci gaban da ba gurbatawa, ba saura, lafiya shiri don rage cutar kokwamba teku yana daya daga cikin zafi spots na yanzu bincike.

Potassium diformate fari ne mai sako-sako da foda, bushe da rashin ɗanɗano.Ita ce ƙarar abinci ta farko wacce ƙungiyar Tarayyar Turai ta amince don maye gurbin maganin rigakafi.Yana iya haɓaka haɓakar dabbobin al'ada, hana haɓakar ƙwayoyin cuta masu cutarwa, da haɓaka yanayin hanji, Potassium diformate na iya haɓaka girma da haɓakar halittun ruwa.

1 Sakamakon gwaji

1.1 Tasirin potassium diformate na abinci mai gina jiki akan girma da tsirar kokwamba na teku Apostichopus japonicus

Matsakaicin ƙimar girma na Apostichopus japonicus ya ƙaru sosai tare da haɓaka abun ciki na potassium diformate na abinci.Lokacin da abun ciki na potassium diformate na abinci ya kai 0.8%, wato, lokacin da abun ciki na potassium diformate na abinci ya kasance 1.0% da 1.2%, ƙayyadaddun ƙimar girma na Apostichopus japonicus ya fi girma fiye da na sauran jiyya, amma babu wani babban bambanci (P). > 0.05) (Table 2-2).Adadin tsira na kokwamba na teku ya kasance 100% a duk ƙungiyoyi.

1.2 Tasirin potassium diformate na abinci mai gina jiki akan alamomin rigakafi na kokwamba Teku Apostichopus japonicus

Idan aka kwatanta da ƙungiyar kulawa, matakan daban-daban na potassium dicarboxylate na iya inganta ƙarfin phagocytic na coelomocytes da samar da O2 - a cikin digiri daban-daban (tebur 2-3).Lokacin da aka ƙara potassium diformate a 1.0% da 1.2%, aikin phagocytic na coelomocytes da samar da nau'in oxygen mai aiki O2 - a cikin kokwamba na teku sun kasance mafi girma fiye da waɗanda ke cikin ƙungiyar kulawa, amma babu wani bambance-bambance tsakanin 1% da 1.2% potassium diformate kungiyoyin, ko tsakanin sauran matakan potassium diformate da kuma kula da kungiyar.Tare da karuwar abun ciki na potassium dicarboxylate a cikin abinci, SOD da NOS na kokwamba na teku sun karu.

1.3 Tasirin Diformate na abinci mai gina jiki akan juriya na kokwamba na teku zuwa kamuwa da cutar Vibrio.

1.4 Kwanaki bayan kalubale, yawan mace-mace na kokwamba na teku a cikin rukunin kulawa shine 46.67%, wanda ya fi girma fiye da haka a cikin 0.4%, 0.6%, 0.8%, 1.0% da 1.2% potassium diformate kungiyoyin (26.67%, 26.67%, 30) %, 30% da 23.33%), amma babu wani gagarumin bambanci tare da 0.2% jiyya kungiyar (38.33%).Yawan mace-mace na kokwamba na teku a cikin 0.4%, 0.6%, 0.8%, 1.0% da 1.2% potassium diformate kungiyoyin ba su da wani gagarumin bambanci.

2. Tattaunawa

2.1 Tasirin potassium dicarboxylate akan ci gaban kokwamba na teku Apostichopus japonicus

A cikin dabbobi, tsarin aikin potassium dicarboxylate shine galibi don shiga gastrointestinal tract, inganta yanayin gastrointestinal, daidaita pH, da kashe kwayoyin cutarwa (Ramli da sunanto, 2005).Bugu da kari, potassium diformate kuma na iya inganta sha na gina jiki a cikin abinci da inganta narkewa da yawan amfanin dabbobin da aka yi amfani da su.A cikin aikace-aikacen dabbobin ruwa, gwaje-gwaje sun nuna cewa potassium diformate na iya inganta girma da kuma rayuwar shrimp sosai (he Suxu, Zhou Zhigang, et al., 2006).A cikin wannan binciken, an inganta ci gaban kokwamba na teku (Apostichopus japonicus) ta hanyar ƙara potassium dicarboxylate don ciyarwa, wanda ya dace da sakamakon aikace-aikacen potassium dicarboxylate a cikin alade da kuma ƙare aladu da aka ruwaito ta hanyar verland.M (2000).

2.2 Tasirin potassium dicarboxylate akan rigakafi na kokwamba na teku Apostichopus japonicus

Apostichopus japonicus yana da tsarin tsaro iri ɗaya kamar sauran echinoderms, wanda aka kammala ta hanyar salon salula da martanin rigakafi.Ana amfani da shi musamman don ganowa da kawar da baƙon da ke shiga jikin dabba, ko sanya na waje su zama abubuwa marasa lahani, da kuma gyara raunuka.Amsar rigakafi ta salula na echinoderms an cika shi da nau'ikan coelomocytes, waɗanda ke samar da tsarin tsaro na echinoderms.Babban ayyuka na wadannan kwayoyin sun hada da phagocytosis, cytotoxin dauki, da kuma samar da antibacterial abubuwa a matakin coagulation (kudriavtsev, 2000).A cikin aiwatar da phagocytosis, coelomocytes na iya haifar da ƙwayoyin cuta ko sassan bangon ƙwayoyin cuta don samar da nau'in oxygen mai amsawa (ROS), gami da babu, H2O2, oh da O2 -.A cikin wannan gwaji, ƙara 1.0% da 1.2% potassium dicarboxylate zuwa abinci yana haɓaka aikin phagocytic na coelomocytes da samar da nau'in oxygen mai amsawa.Koyaya, tsarin potassium diformate yana haɓaka ayyukan phagocytic da O2 - samarwa yana buƙatar ƙarin nazarin.

2.3 Tasirin potassium dicarboxylate akan furen hanji na kokwamba teku Apostichopus japonicus

Potassium dicarboxylate za a iya bazu zuwa cikin formic acid da formate a cikin rauni alkaline yanayi da kuma shiga cikin microbial Kwayoyin ta cikin cell membrane.Yana iya canza yanayin rayuwa na ƙwayoyin cuta masu cutarwa kamar Escherichia coli da Salmonella ta hanyar canza ƙimar pH a cikin sel da hana haifuwar su, don daidaita ma'aunin microecological na hanji (eidelsburger, 1998).Tasirin potassium dicarboxylate akan microflora na hanji, macroscopically, H + da aka samar ta hanyar bazuwar potassium dicarboxylate yana rage ƙimar pH a cikin hanji kuma yana hana haɓakar microflora na hanji.Ta hanyar microscopically, H + yana shiga cikin ƙwayoyin ƙwayoyin cuta ta hanyar membrane cell, yana lalata ayyukan enzymes na ciki kai tsaye, yana tasiri metabolism na furotin microbial da acid nucleic, kuma yana taka rawa wajen haifuwa (Roth, 1998).Sakamakon ya nuna cewa potassium diformate yana da ɗan tasiri a kan jimlar ƙwayoyin hanji na kokwamba na teku, amma yana iya hana yawan Vibrio.

2.4 Tasirin potassium dicarboxylate akan jure cututtuka na kokwamba teku Apostichopus japonicus

Vibrio splendens shine cututtukan cututtukan fata na cututtukan fata na kokwamba, wanda ke cutar da samarwa da noman kokwamba na teku.Wannan gwaji ya tabbatar da cewa ƙara potassium dicarboxylate a cikin abinci ya rage yawan mace-macen kokwamba na teku da ke kamuwa da Vibrio mai haske.Wannan na iya kasancewa yana da alaƙa da tasirin hanawa na potassium diformate akan Vibrio.

3 Kammalawa

Sakamakon ya nuna cewa potassium diformate na abinci mai gina jiki yana da tasiri mai mahimmanci akan ci gaban Apostichopus japonicus, yana da tasiri mai kyau akan ƙayyadaddun rigakafi na Apostichopus japonicus, kuma ya inganta rigakafi da salon salula na Apostichopus japonicus.Bugu da kari na potassium dicarboxylate a cikin rage cin abinci muhimmanci rage yawan cutarwa kwayoyin cuta a cikin hanji na teku kokwamba, da kuma inganta cutar juriya na kokwamba teku kamuwa da Vibrio m.A ƙarshe, ana iya amfani da potassium dicarboxylate azaman mai haɓaka rigakafi a cikin abincin kokwamba na teku, kuma adadin da ya dace na potassium dicarboxylate shine 1.0%.