Ka ho atolosoa ha sekala sa setso le ho eketseha ha setso sa setso, lefu la Apostichopus japonicus le se le le bohlokoa haholo, le tlisitseng tahlehelo e kholo indastering ea temo ea metsing.Mafu a Apostichopus japonicus a bakoa haholo-holo ke libaktheria, likokoana-hloko le li-ciliates, tseo har'a tsona lefu la ho bola ha letlalo le bakoang ke Vibrio brilliant ke lona le tebileng ka ho fetisisa.Ka ho mpefala ha lefu lena, lerako la 'mele la Apostichopus japonicus ulcers, le etsa matheba a maputsoa le a masoeu,' me qetellong le itšehla thajana ho fihlela lefung, le qhibiliha ho ba mamina a nko joalo ka colloid.Ka thibelo ea mafu a setso le kalafo, lithibela-mafu li sebelisoa haholo.Empa tšebeliso ea nako e telele ea lithibela-mafu ha e na kotsi e patiloeng ea ho hanyetsa baktheria le masala a lithethefatsi, empa hape e tlisa polokeho ea lijo le tšilafalo ea tikoloho.Ka hona, nts'etsopele ea sebaka se sa silafatsang, se se nang masala, boitokisetso bo sireletsehileng ho fokotsa lefu la likomkomere tsa leoatle ke se seng sa libaka tse chesang tsa lipatlisiso tsa hajoale.

Potassium diformate ke phofo e hlephileng e tšoeu ea kristale, e omileng ebile e se na tatso.Ke motsoako oa pele oa phepelo ea lithibela-mafu o amohetsoeng ke European Union ho nka sebaka sa lithibela-mafu.E ka khothaletsa kholo ea liphoofolo tse ruiloeng, ea thibela kholo ea libaktheria tse kotsi, 'me ea ntlafatsa tikoloho ea mala, Potassium diformate e ka ntlafatsa haholo kholo le chai ea lintho tse phelang metsing.

1 Liphetho tsa liteko

1.1 Liphello tsa potassium ea lijo li senya kholo le ho phela ha likomkomere tsa leoatle Apostichopus japonicus

Sekhahla se ikhethileng sa kholo ea Apostichopus japonicus se eketsehile haholo ka keketseho ea lijo tsa potassium diformate content.Ha lijo tse nang le potassium diformate li fihla ho 0.8%, ke hore, ha lijo tse nang le potassium diformate li ne li le 1.0% le 1.2%, sekhahla se khethehileng sa kgolo ea Apostichopus japonicus se ne se phahame haholo ho feta sa phekolo e meng, empa ho ne ho se na phapang e khōlō (P. > 0.05) (tafole 2-2).Sekhahla sa ho phela ha likomkomere tsa leoatle e ne e le 100% lihlopheng tsohle.

1.2 Litlamorao tsa potassium diformate ho li-immune indexes tsa likomkomere tsa leoatle Apostichopus japonicus

Ha ho bapisoa le sehlopha sa taolo, maemo a fapaneng a potassium dicarboxylate a ka ntlafatsa bokhoni ba phagocytic ba coelomocytes le tlhahiso ea O2 - ka likhato tse fapaneng (tafole 2-3).Ha potassium diformate e eketsoa ka 1.0% le 1.2%, ts'ebetso ea phagocytic ea coelomocyte le tlhahiso ea mefuta ea oksijene e sebetsang O2 - ka likomkomereng tsa leoatle e ne e phahame haholo ho feta ea sehlopha sa taolo, empa ho ne ho se na phapang e khōlō pakeng tsa 1% le 1.2% potassium diformate lihlopha, kapa pakeng tsa maemo a mang a potassium diformate le sehlopha taolo.Ka ho eketseha ha potasiamo dicarboxylate lijong, SOD le NOS ea likomkomere tsa leoatle li ile tsa eketseha.

1.3 Phello ea phepo ea potasiamo e senya ho hanyetsa ha likomkomere tsa leoatleng ho tšoaetso ea Vibrio brilliant

Matsatsi a 1.4 ka mor'a phephetso, palo ea ho shoa ha likomkomere tsa leoatleng sehlopheng sa taolo e bile 46.67%, e neng e phahame haholo ho feta ea lihlopha tsa 0.4%, 0.6%, 0.8%, 1.0% le 1.2% potassium diformate (26.67%, 26.67%, 30% %, 30% le 23.33%), empa ho ne ho se na phapang e khōlō le sehlopha sa phekolo sa 0.2% (38.33%).Ho shoa ha likomkomere tsa leoatleng ho 0.4%, 0.6%, 0.8%, 1.0% le 1.2% ea lihlopha tsa potassium diformate li ne li se na phapang e khōlō.

2. Puisano

2.1 Phello ea potassium dicarboxylate khōlong ea likomkomere tsa leoatle Apostichopus japonicus

Liphoofolo, mokhoa oa ts'ebetso oa potassium dicarboxylate ke haholo-holo ho kena ka mpeng ea mala, ho ntlafatsa tikoloho ea mala, ho laola pH, le ho bolaea libaktheria tse kotsi (Ramli le sunanto, 2005).Ho feta moo, potassium diformate e ka boela ea khothalletsa ho monya ha limatlafatsi lijong le ho ntlafatsa tšilo ea lijo le sekhahla sa tšebeliso ea liphoofolo tse ruiloeng.Tšebelisong ea liphoofolo tse phelang metsing, liteko li bontšitse hore potassium diformate e ka ntlafatsa haholo kholo le lebelo la ho phela ha li-shrimp (he Suxu, Zhou Zhigang, et al., 2006).Thutong ena, kholo ea likomkomere tsa leoatle (Apostichopus japonicus) e ile ea khothaletsoa ka ho kenyelletsa potassium dicarboxylate ho fepa, e neng e lumellana le liphello tsa potassium dicarboxylate kopo ho likolobe le likolobe tse qetellang tse tlalehiloeng ke verland.M (2000).

2.2 Phello ea potassium dicarboxylate ho itšireletsa ha likomkomere tsa leoatle Apostichopus japonicus

Apostichopus japonicus e na le mokhoa o ts'oanang oa ts'ireletso joaloka li-echinoderms tse ling, tse phethoang ke karabelo ea 'mele ea ho itšireletsa mafung ea cellular le e seng ea cellular (humoral).Haholo-holo e sebelisetsoa ho khetholla le ho felisa lihlopha tse tsoang linaheng tse ling tse kenang 'meleng oa liphoofolo, kapa ho etsa hore 'mele oa linaha tse ling e be lintho tse se nang kotsi, le ho lokisa maqeba.Karabelo ea 'mele ea ho itšireletsa mafung ea li-echinoderms e phethoa ke mefuta e sa tšoaneng ea li-coelomocyte, tse etsang mokhoa oa ho itšireletsa oa echinoderms.Mesebetsi ea mantlha ea lisele tsena e kenyelletsa phagocytosis, cytotoxin reaction, le tlhahiso ea lintho tse thibelang likokoana-hloko maemong a coagulation (kudriavtsev, 2000).Ts'ebetsong ea phagocytosis, li-coelomocyte li ka susumetsoa ke libaktheria kapa likarolo tsa lebota la baktheria ho hlahisa mefuta ea oksijene e sebetsang (ROS), ho kenyelletsa le che, H2O2, oh le O2 -.Tekong ena, ho kenyelletsa 1.0% le 1.2% potassium dicarboxylate lijong ho ekelitse ts'ebetso ea phagocytic ea li-coelomocyte le tlhahiso ea mefuta ea oksijene e sebetsang.Leha ho le joalo, mochine oa potassium diformate o eketsa mosebetsi oa phagocytic le O2 - tlhahiso e hloka ho tsoela pele ho ithutoa.

2.3 Phello ea potassium dicarboxylate limela tsa mala a likomkomere tsa leoatle Apostichopus japonicus

Potassium dicarboxylate e ka aroloa ho ba formic acid le ho theha tikolohong e fokolang ea alkaline ebe e kena liseleng tsa microbial ka lera la sele.E ka fetola tikoloho ea bophelo ea likokoana-hloko tse kotsi tse kang Escherichia coli le Salmonella ka ho fetola boleng ba pH ka har'a lisele le ho thibela ho ikatisa ha tsona, e le ho laola ho leka-lekana ha microecological ea mala (eidelsburger, 1998).Phello ea potassium dicarboxylate ho microflora ea mala, macroscopically, H + e hlahisoang ke ho bola ha potassium dicarboxylate e fokotsa boleng ba pH ka maleng le ho thibela kholo ea mala a microflora.Ka microscopically, H + e kena ka har'a lisele tsa baktheria ka lera la sele, e senya ka ho toba mosebetsi oa li-enzyme tsa intracellular, e ama metabolism ea protheine ea likokoana-hloko le nucleic acid, 'me e phetha karolo ea ho etsa sterilization (Roth, 1998).Liphetho li bonts'itse hore potassium diformate e na le phello e fokolang ho libaktheria tsohle tsa mala a likomkomere tsa leoatleng, empa e ka sitisa palo ea Vibrio haholo.

2.4 Phello ea potassium dicarboxylate ho hanyetsa mafu a likomkomere tsa leoatle Apostichopus japonicus

Vibrio splendens ke baktheria ea pathogenic ea letlalo la likomkomere tsa leoatle, e leng kotsi ho hlahisa le ho lema likomkomere tsa leoatle.Teko ena e bonts'itse hore ho kenyelletsa potassium dicarboxylate lijong ho fokolitse ho shoa ha likomkomere tsa leoatleng tse tšoaelitsoeng ke Vibrio brilliant.Sena se ka amahanngoa le phello e thibelang ea potassium diformate ho Vibrio.

3 Qetello

Liphetho li bonts'itse hore potassium diformate ea lijo e bile le phello e kholo kholong ea Apostichopus japonicus, e bile le phello e ntle ho boits'ireletso bo sa tobang ba Apostichopus japonicus, mme ea matlafatsa boits'ireletso ba mmele le lisele tsa Apostichopus japonicus.Ho eketsoa ha potassium dicarboxylate lijong ho fokolitse palo ea libaktheria tse kotsi ka maleng a likomkomere tsa leoatle, 'me ho matlafalitse ho hanyetsa lefu la likomkomere tsa leoatleng tse tšoaelitsoeng ke Vibrio brilliant.Qetellong, potasiamo dicarboxylate e ka sebelisoa e le se matlafatsang sesole sa 'mele lijong tsa likomkomere tsa leoatle,' me tekanyo e nepahetseng ea potassium dicarboxylate ke 1.0%.