Ukushintsha ingxenye ye-fishmeal nge-soybean meal (SBM) njengendlela eqhubekayo nezomnotho iye yahlolwa ezinhlotsheni eziningi zezilwane zasemanzini ezihloselwe ukuhweba, okuhlanganisa ne-freshwater rainbow trout (I-Oncorhynchus mykiss).Kodwa-ke, i-soy nezinye izinto ezisekelwe ezitshalweni ziqukethe amazinga aphezulu e-saponins nezinye izici eziphikisana nokudla okunomsoco ezibangela i-subacute enteritis ye-distal intestine eziningi zalezi zinhlanzi.Lesi simo sibonakala ngokwanda kokungena kwamathumbu emathunjini, ukuvuvukala, kanye nokungajwayelekile kwe-morphological okuholela ekwehleni kokusebenza kahle kokuphakelayo kanye nokukhubazeka kokukhula.

Ku-rainbow trout, okuhlanganisa i-SBM engaphezu kwama-20% okudla kukhonjiswe ukuthi kubangele i-soy-enteritis, okubeka umkhawulo wokuphila ezingeni elingashintshwa libe ukudla okujwayelekile kwe-aquaculture.Ucwaningo lwangaphambilini luhlole izindlela eziningi zokulwa nalesi sifo, okuhlanganisa ukuxhashazwa kwe-gut microbiome, ukucubungula isithako ukuze kususwe izici eziphikisana nokudla okunomsoco, kanye nezithasiselo ze-antioxidant kanye nama-probiotic.Indlela eyodwa engahloliwe ukufakwa kwe-trimethylamine oxide (TMAO) kokuphakelayo kwezilwane zasemanzini.I-TMAO iyi-cytoprotectant yendawo yonke, eqoqwe ezinhlotsheni eziningi njengeprotheni kanye ne-membrane stabilizer.Lapha, sihlola ikhono le-TMAO lokuthuthukisa ukuzinza kwe-enterocyte futhi icindezele isignali ye-HSP70 evuthayo ngaleyo ndlela ilwe ne-enteritis ebangelwa isoya futhi kuholele ekwengezeni ukusebenza kahle kokuphakelayo, ukugcinwa kanye nokukhula kwe-trout yamanzi ahlanzekile.Ngaphezu kwalokho, sihlola ukuthi i-marine fish solubles, umthombo ocebile we-TMAO, ingasetshenziswa njengendlela engokomnotho yokuphatha lesi sengezo, esivumela ukusetshenziswa kwayo esikalini sokuhweba.

I-rainbow trout efuywayo (i-Troutlodge Inc.) yayinesisindo esivamile esingama-40 g kanye no-n=15 ithangi ngalinye emathangini okwelapha aphindwe kathathu.Amathangi aphiwa ukudla okukodwa kokuyisithupha okulungiselelwe ngokwesisekelo somsoco ogayekayo ohlinzeka ngamaprotheni angu-40% agayekayo, u-15% wamafutha angcolile, futhi ahlangabezana nokugxiliswa kwe-amino acid efanelekile.Ukudla kwakuhlanganisa ukudla kwenhlanzi 40 control (% yokudla okomile), SBM 40, SBM 40 + TMAO 3 g kg^-1, SBM 40 + TMAO 10 g kg^-1, SBM 40 + TMAO 30 g kg^-1, kanye ne-SBM 40 + 10% yezinto ezincibilikayo zezinhlanzi.Amathangi adliswa kabili nsuku zonke ukuze asuthe okubonakala amasonto angu-12 kanye nokuhlaziywa kwe-fecal, proximate, histological and molecular eyenziwa.

Imiphumela yalolu cwaningo kuzoxoxwa ngayo kanye nokusetshenziswa kokufaka i-TMAO ukuze kusetshenziswe ukusetshenziswa okuphezulu kwemikhiqizo yesoya yase-US kuma-aquafeeds e-salmonid.